2022
DOI: 10.2147/ijn.s346141
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Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency

Abstract: Purpose Transplantation of stem cells to remodel the trabecular meshwork (TM) has become a new option for restoring aqueous humor dynamics and intraocular pressure homeostasis in glaucoma. In this study, we aimed to design a nanoparticle to label induced pluripotent stem cell (iPSC)-derived TM and improve the delivery accuracy and in vivo tracking efficiency. Methods PLGA-SPIO-Cypate (PSC) NPs were designed with polylactic acid-glycolic acid (PLGA) polymers as the backb… Show more

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Cited by 8 publications
(7 citation statements)
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References 73 publications
(73 reference statements)
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“…The trabecular meshwork cells can mediate the regulation of intraocular pressure by regulating aqueous humor flow ( Zhu et al, 2017 ). Wang et al (2022) designed a nanoparticle to label trabecular meshwork cells, and the use of nanoparticles not only facilitated long-term tracking, but also improved the transmission accuracy of transplanted cells in vivo experiments ( Wang et al, 2022 ). It is more important that the use of magnet temporarily enhanced the effectiveness of cell-based therapy in glaucoma-related pathology, and promoted the clinical transformation of stem cell-based glaucoma therapy ( Zhu et al, 2016 ; Wang et al, 2022 ).…”
Section: Discussionmentioning
confidence: 99%
“…The trabecular meshwork cells can mediate the regulation of intraocular pressure by regulating aqueous humor flow ( Zhu et al, 2017 ). Wang et al (2022) designed a nanoparticle to label trabecular meshwork cells, and the use of nanoparticles not only facilitated long-term tracking, but also improved the transmission accuracy of transplanted cells in vivo experiments ( Wang et al, 2022 ). It is more important that the use of magnet temporarily enhanced the effectiveness of cell-based therapy in glaucoma-related pathology, and promoted the clinical transformation of stem cell-based glaucoma therapy ( Zhu et al, 2016 ; Wang et al, 2022 ).…”
Section: Discussionmentioning
confidence: 99%
“…As described previously, 22 the cultured medium conditioned by human trabecular meshwork cells was pooled and sterilised by filtration through mixed cellulose ester membrane filters (0.2 μm pore size; Millipore, Danvers, MA) and applied to induced mouse iPSCs differentiation for 10 days. Differentiated iPSCs, named iPSC‐TM, were separated from undifferentiated cells using a magnetic bead‐based approach 22 and characterised as TM‐like cells since the expressions of TM biomarkers and dexamethasone‐induced myocilin expression and cross‐linked actin network formation 7,21–25 . iPSC‐TM generated following this protocol was used in this study.…”
Section: Methodsmentioning
confidence: 99%
“…Moreover, our previous studies have revealled that endogenous cells can transit from a quiescent state to an active status immediately after interacting with induced pluripotent stem cell‐derived TM (iPSC‐TM) cells. This initial activation at the time of transplantation enables the TM to regenerate efficiently for an extended period, even though iPSC‐TM cells diminished after transplantation 7,21–25 . Thus, it is of particular interest to investigate the features of these highly responsive cells.…”
Section: Introductionmentioning
confidence: 99%
“…MNPs have been used to accurately deliver induced pluripotent stem cells (iPSCs) to the trabecular meshwork in vivo ; similar methods could potentially be used for iPSC-derived RPE delivery. 30 …”
Section: Therapeutics: Drugs Delivery Systems and Targetingmentioning
confidence: 99%