Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation

Hou, Ruixia; Zhang, Guohua; Du, Gaolai; Zhan, Dan-Xia; Cong, Yang; Cheng, Ya‐Jun; Fu, Jun

doi:10.1016/j.colsurfb.2012.10.067

Cited by 100 publications

(72 citation statements)

References 30 publications

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“…On PVA-HA composites, the adhesion and growth of human SV40 osteoblasts is enhanced up to HA concentration of 50 wt%, and then decreases. 28 HA-titanium composites with 5 and 10 wt% of Ti give more support than pure HA to the viability and growth of Saos-2 cells. 43 One can expect that the cytotoxicity of HA at higher concentrations is mediated by a relatively high release of Ca 2+ ions, which are known to have a negative effect on osteoblastic cells in vitro.…”

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confidence: 96%

“…Similarly, in the study with PVA-HA composites, the compressive strength was shown to reach its maximum in the material with 10 wt% of HA, and then decrease with increasing concentration of HA. 28 In addition, nano-sized HA powder was more evenly dispersed throughout our composite, which is advantageous for in vivo applications. 22 In accordance with this, the compressive modulus of HA-PCL composites with HA nanoparticles was higher than the compressive modulus of HA-PCL composites with HA microparticles, because the nanoparticles were more uniformly dispersed within the material.…”

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confidence: 96%

“…For example, admixing HA nanoparticles into the poly-(vinyl alcohol) (PVA) hydrogel increases the adhesion and proliferation of human SV40 osteoblasts compared with the pure hydrogel, and this effect is further enhanced by combining HA with Fe 2 O 3 . 28 Similarly, in a composite material made of poly(ε-caprolactone) (PCL) matrix containing 10, 20, or 30 wt% of iron-doped HA, nanoparticles support the viabil- …”

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Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Filová¹,

Suchý²,

Sucharda³

et al. 2014

IJN

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Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.

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confidence: 96%

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confidence: 96%

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Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Filová¹,

Suchý²,

Sucharda³

et al. 2014

IJN

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“…For instance, the incorporation of osteoconductive hydroxyapatite particles into PVA hydrogel films has been shown to enhance cell adhesion to PVA hydrogels and stimulate osteoblast proliferation (30,31). As an illustration, coatings of hydroxyapatite particles were produced by swelling-induced gelation on the same spherical PAAc-PAAm substrates as the ones studied above.…”

Section: Application Potential Of Hydrogel Film Fabrication By Swellimentioning

confidence: 99%

Hydrogel films and coatings by swelling-induced gelation

Moreau

Chauvet

François

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Hydrogel films used as membranes or coatings are essential components of devices interfaced with biological systems. Their design is greatly challenged by the need to find mild synthesis and processing conditions that preserve their biocompatibility and the integrity of encapsulated compounds. Here, we report an approach to produce hydrogel films spontaneously in aqueous polymer solutions. This method uses the solvent depletion created at the surface of swelling polymer substrates to induce the gelation of a thin layer of polymer solution. Using a biocompatible polymer that self-assembles at high concentration [poly(vinyl alcohol)], hydrogel films were produced within minutes to hours with thicknesses ranging from tens to hundreds of micrometers. A simple model and numerical simulations of mass transport during swelling capture the experiments and predict how film growth depends on the solution composition, substrate geometry, and swelling properties. The versatility of the approach was verified with a variety of swelling substrates and hydrogel-forming solutions. We also demonstrate the potential of this technique by incorporating other solutes such as inorganic particles to fabricate ceramic-hydrogel coatings for bone anchoring and cells to fabricate cell-laden membranes for cell culture or tissue engineering. H ydrogel films are used in a number of biomedical applications like wound healing (1), drug delivery (1), antiadhesive membranes (2), soft-tissue reconstruction (3, 4), and cell culture (5). The quality of their interactions with biological tissues or entrapped cells is central to the performance of these systems. Although considerable advances have been made in synthesis (6) and processing (7) to tailor these interactions, the toxicity of by-products and the degradation caused by harsh processing conditions remain key issues (8), which can be overcome (9) but are often addressed at the cost of difficult synthesis or multistep processes. There is a great interest for simple, versatile, and in vivo-friendly methods to fabricate and modify hydrogels. We propose a strategy to design hydrogel films in mild conditions. This method could be used to produce freestanding hydrogel membranes or hydrogel coatings to functionalize other hydrogels.Our approach relies on the understanding of transport phenomena occurring near the surface of a polymer network swelling in a polymer solution. As an illustration, let us consider a flat polymer network with thickness H 0 immersed in a solution containing a volume fraction Φ 0 of free polymer chains, as depicted in Fig. 1A. When the solvent of the solution is also a solvent for the network, the network swells in a diffusive process. For early immersion times t, the increase in thickness ΔH is given by the following (10, 11):where H ∞ is the thickness at equilibrium swelling in the polymer solution and D 0 is a diffusivity coefficient depending on the dynamics of the network and of the solution. The systems of interest are those where the chains in solution do not penetrate...

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“…17 During the synthesis of nanomaterials, some polymers like polyvinyl pyrrolidone (PVP), polyvinyl alcohol, polystyrene, and dextran control the size, shape, and growth of nanoparticles. [18][19][20][21][22][23][24][25][26][27] Liu et al used PVP as a surfactant in one-pot polyol process to synthesize mono-size PVP-coated iron oxide nanoparticles for biomedical application. 18 Sun et al reported the synthesis of polyethylene glycol (PEG)-coated iron oxide nanoparticle for specific targeting of glioma tumors via the surface-bound targeting peptide, chlorotoxin.…”

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Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Mondal¹,

Manivasagan²,

Bharathiraja³

et al. 2017

IJN

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In this review, specific attention is paid to the development of nanostructured magnetic hydroxyapatite (MHAp) and its potential application in controlled drug/gene delivery, tissue engineering, magnetic hyperthermia treatment, and the development of contrast agents for magnetic resonance imaging. Both magnetite and hydroxyapatite materials have excellent prospects in nanomedicine with multifunctional therapeutic approaches. To date, many research articles have focused on biomedical applications of nanomaterials because of which it is very difficult to focus on any particular type of nanomaterial. This study is possibly the first effort to emphasize on the comprehensive assessment of MHAp nanostructures for biomedical applications supported with very recent experimental studies. From basic concepts to the real-life applications, the relevant characteristics of magnetic biomaterials are patented which are briefly discussed. The potential therapeutic and diagnostic ability of MHAp-nanostructured materials make them an ideal platform for future nanomedicine. We hope that this advanced review will provide a better understanding of MHAp and its important features to utilize it as a promising material for multifunctional biomedical applications.

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Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation

Cited by 100 publications

References 30 publications

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Hydrogel films and coatings by swelling-induced gelation

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

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