Magnetic Nanoparticle-Embedded Hydrogel Sheet with a Groove Pattern for Wound Healing Application

Abstract

Endothelial progenitor cells (EPCs) can induce a pro-angiogenic response during tissue repair. Recently, EPC transplantations have been widely investigated in wound healing applications. To maximize the healing efficacy by EPCs, a unique scaffold design that allows cell retention and function would be desirable for in situ delivery. Herein, we fabricated an alginate/poly-L-ornithine/gelatin (alginate-PLO-gelatin) hydrogel sheet with a groove pattern for use as a cell delivery platform. In addition, we demonstrate the topographical modification of the hydrogel sheet surface with a groove pattern to modulate cell proliferation, alignment, and elongation. We report that the patterned substrate prompted morphological changes of endothelial cells, increased cell−cell interaction, and resulted in the active secretion of growth factors such as PDGF-BB. Additionally, we incorporated magnetic nanoparticles (MNPs) into the patterned hydrogel sheet for the magnetic field-induced transfer of cellseeded hydrogel sheets. As a result, enhanced wound healing was observed via efficient transplantation of the EPCs with an MNP-embedded patterned hydrogel sheet (MPS). Finally, enhanced vascularization and dermal wound repair were observed with EPC seeded MPS.

Recent progress of magnetic nanoparticles in biomedical applications: A review

“…[ 145 ] Apart from the co‐delivery of lipophilic and hydrophilic drugs, magnetic nanoparticles embedded hydrogen has been proven to be successful in wound healing applications. [ 146 ] A groove pattern scaffold was fabricated by alginate/poly‐ l ‐ornithine/gelatin (alginate‐PLO‐gelatin) hydrogel sheet for cell delivery to the wound site. This grove pattern helps with cell proliferation, alignment, and elongation.…”

“…This grove pattern helps with cell proliferation, alignment, and elongation. [ 146 ] Additionally, the investigators embedded magnetic nanoparticles in the groove pattern to facilitate magnetic field induced transfer of cell seeded hydrogel sheet to the wounded site. Table 3 summarizes the recent development of magnetic‐hydrogel nanocomposite and their usage as drug delivery module.…”

Advanced Bottom‐Up Engineering of Living Architectures

“…Also, cells internalizing magnetic nanoparticles can be forced to assemble over different surfaces to create cell sheet‐like structures . More recently, Park and co‐workers embedded magnetic nanoparticles in thin hydrogel sheets for efficient harvesting of endothelial progenitor cell sheets . Using a bioinspired approach, researchers have also developed a cellulose‐dopamine coating which enabled cellulase‐assisted enzymatic harvesting with minimal cell damage as recently reported …”

Carbon coated Nickel Nanoparticles in Polyacrylamide Ferrogels: Interaction with Polymeric Network and Impact on Swelling

“…One of the main advantages of ferrogels over traditional stimulus-responsive polymers is that they can be remotely activated by a non-contact force (magnetic field). This unique property makes ferrogels prospective advanced material in various fields such as drug delivery [6,7], soft robotics [8], tissue reconstruction [9,10] and environmental engineering [11,12].…”