Magnetic nanoparticles as versatile carriers for enzymes immobilization: A review

Bilal, Muhammad; Zhao, Yuping; Rasheed, Tahir; Iqbal, Hafiz M.N.

doi:10.1016/j.ijbiomac.2018.09.025

Cited by 387 publications

(183 citation statements)

References 86 publications

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“…Numerous methods have been implemented for enzyme immobilization, taking into account the intended application [12]. In this sense, magnetic nanoparticles (MNPs) have received increasing attention as enzyme carriers for biotechnological and biomedical applications [13].MNPs can easily be recovered from aqueous solutions using an external magnetic field and exhibit useful properties such as high surface area-to-volume ratio, making possible an increase in the enzyme density, and the possibility to be surface modified [14]. In our study, we used a non-covalent site-specific immobilization of the enzyme, as this method uses mild conditions.…”

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confidence: 99%

CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

et al. 2020

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D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.

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confidence: 99%

CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

et al. 2020

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“…Considering their pharmaceutical applications, IOMNPs and their clusters and assemblies must show colloidal stability and biocompatibility in various biological environments [3,12,13,16]. Small molecules, biomacromolecules, and polymers can be utilized as ligands to mediate IOMNP assemblies [7,9,57]. However, small-molecular ligands generally show low stability and biocompatibility, and it is hard to find a small-molecular ligand [11,59].…”

Section: Iomnp Assemblies and Clustersmentioning

confidence: 99%

“…In the last few decades, the use of iron-oxide nanoparticles displaying magnetic properties attracted great interest in many application areas, from magnetic recording media to pharmaceutical applications such as therapy and drug delivery [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Each application of these nanoparticles needs specific and, sometimes, different properties [1,12,15].…”

Section: Introductionmentioning

confidence: 99%

Pharmaceutical Applications of Iron-Oxide Magnetic Nanoparticles

Bruschi

Toledo

2019

Magnetochemistry

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Advances of nanotechnology led to the development of nanoparticulate systems with many advantages due to their unique physicochemical properties. The use of iron-oxide magnetic nanoparticles (IOMNPs) in pharmaceutical areas increased in the last few decades. This article reviews the conceptual information about iron oxides, magnetic nanoparticles, methods of IOMNP synthesis, properties useful for pharmaceutical applications, advantages and disadvantages, strategies for nanoparticle assemblies, and uses in the production of drug delivery, hyperthermia, theranostics, photodynamic therapy, and as an antimicrobial. The encapsulation, coating, or dispersion of IOMNPs with biocompatible material(s) can avoid the aggregation, biodegradation, and alterations from the original state and also enable entrapping the bioactive agent on the particle via adsorption or covalent attachment. IOMNPs show great potential for target drug delivery, improving the therapy as a consequence of a higher drug effect using lower concentrations, thus reducing side effects and toxicity. Different methodologies allow IOMNP synthesis, resulting in different structures, sizes, dispersions, and surface modifications. These advantages support their utilization in pharmaceutical applications, and getting suitable drug release control on the target tissues could be beneficial in several clinical situations, such as infections, inflammations, and cancer. However, more toxicological clinical investigations about IOMNPs are necessary.

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Magnetic separation processes are known as integrated bioanalytical protein purification method since decades and are well described. Typically, magnetic separation is used for purification tasks like enzyme, cell [5] as well as protein purification [6][7][8], such as the recovery of immunoglobulins from cell supernatants or serum [9,10], recycling of immobilized enzymes [11][12][13][14][15], as well as cell labeling and sorting [16][17][18]. With the aim of overcoming this prejudice, a comprehensive process development approach is presented, based on a GMP-compliant magnetic separator, including an optimization of the batch adsorption process, implementation into a technical-scale, and the development and validation of cleaning routines for the device.

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mentioning

confidence: 99%

“…Magnetic separation is a highly flexible technique that has been applied for various applications in biotechnology. Typically, magnetic separation is used for purification tasks like enzyme, cell [5] as well as protein purification [6][7][8], such as the recovery of immunoglobulins from cell supernatants or serum [9,10], recycling of immobilized enzymes [11][12][13][14][15], as well as cell labeling and sorting [16][17][18]. The use of MP is well established and routinely used in industry and is constantly opening up new fields of applications in academia Eng Life Sci.…”

mentioning

confidence: 99%

First comprehensive view on a magnetic separation based protein purification processes: From process development to cleaning validation of a GMP‐ready magnetic separator

Ebeler

Pilgram

Wellhöfer

et al. 2019

Engineering in Life Sciences

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Magnetic separation processes are known as integrated bioanalytical protein purification method since decades and are well described. However, use of magnetic separation processes in a regulated industrial production environment has been prevented by the lack of suitable process equipment and prejudice against the productivity of the process and its qualification for cleaning‐in‐place operation. With the aim of overcoming this prejudice, a comprehensive process development approach is presented, based on a GMP‐compliant magnetic separator, including an optimization of the batch adsorption process, implementation into a technical‐scale, and the development and validation of cleaning routines for the device. By the implementation of a two‐step counter‐current binding process, it was possible to raise the yields of the magnetic separation process even for very low concentrated targets in a vast surplus of competing proteins, like the hormone equine chorionic gonadotropin in serum, from 74% to over 95%. For the validation of the cleaning process, a direct surface swabbing method combined with a total organic carbon analysis was established for the determination of two model contaminants. The cleanability of the process equipment was proven for both model contaminants by reliably meeting the 10 ppm criteria.

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Magnetic nanoparticles as versatile carriers for enzymes immobilization: A review

Cited by 387 publications

References 86 publications

CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

Pharmaceutical Applications of Iron-Oxide Magnetic Nanoparticles

First comprehensive view on a magnetic separation based protein purification processes: From process development to cleaning validation of a GMP‐ready magnetic separator

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