Magnetic nanoparticles-based electrochemical aptasensor for the detection of enrofloxacin in chicken

Guo, Xingyuan; Li, Fang; Zhu, Xueying; Zhang, Pengying; Liu, Yunhong; Li, Zhaozhou

doi:10.1016/j.ijoes.2024.100537

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2024

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Cited by 2 publications

References 32 publications

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Exo Ⅲ-assisted amplification signal strategy synergized with Au@Pt NFs/CoSe2 for sensitive detection of enrofloxacin

Cui,

Lv,

Zhao

et al. 2024

Bioelectrochemistry

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Exo Ⅲ-assisted amplification signal strategy synergized with Au@Pt NFs/CoSe2 for sensitive detection of enrofloxacin

Cui,

Lv,

Zhao

et al. 2024

Bioelectrochemistry

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Fluorescence detection for enrofloxacin by using Aptamer and fluorophore SYBR Green I

Li,

Kong,

Cui

et al. 2024

Preprint

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Enrofloxacin (ENR), as a type of antimicrobial, is commonly used in animal sterilization. However, excessive ENR residues in food will seriously harm human health. Therefore, in this work, we developed a quantitative fluorescence method for ENR based on aptamer and SYBR Green I (SG-I). DNA aptamers with G-quadruplex space structure can be recognized and inserted by SG-I to emit intense green fluorescence. In the presence of ENR, the aptamer preferentially recognized and bound specifically to ENR resulting in the change of aptamer conformation, which prevented the SG-I from inserting into the aptamer to cause fluorescence quenching. The aptamer sensor can quantitatively detect ENR with linear concentration range of 0-600 nM, and the limit of detection was 0.158 nM. The aptamer sensor was used detecting ENR in chicken, fish and pork samples with recovery rate from 99.74–104.02%, and relative standard deviation (RSD) of ENR of less than 6%. Therefore, this aptamer-based sensor provided the fluorescence detection method for ENR in food safety.

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Magnetic nanoparticles-based electrochemical aptasensor for the detection of enrofloxacin in chicken

Cited by 2 publications

References 32 publications

Exo Ⅲ-assisted amplification signal strategy synergized with Au@Pt NFs/CoSe2 for sensitive detection of enrofloxacin

Exo Ⅲ-assisted amplification signal strategy synergized with Au@Pt NFs/CoSe2 for sensitive detection of enrofloxacin

Fluorescence detection for enrofloxacin by using Aptamer and fluorophore SYBR Green I

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