Magnetic Nanoparticles for Local Drug Delivery Using Magnetic Implants

Fernández-Pacheco, Rodrigo; Valdivia, J. G.; Ibarra, M. R.

doi:10.1007/978-1-59745-483-4_35

Cited by 20 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16 Accumulating research findings have shown that nanotechnology and nanoparticles are becoming a focus for application in clinical medicine because of their stable biomolecular absorption, high surface energy, and small size comparable with biomolecules. 27,28 Our present study shows that gambogic acid can inhibit Figure 1B), indicating that magnetic nanoparticles containing Fe 3 O 4 themselves had no cytotoxicity, which is consistent with current research. 29 Our study also provides evidence that the apoptosis ratio in LOVO cells treated with an optimal combination of gambogic acid and Fe 3 O 4 magnetic nanoparticles increased dramatically compared with cells treated with gambogic acid alone (P , 0.05, Figure 2), which confirms that magnetic nanoparticles containing Fe 3 O 4 and gambogic acid have a synergistic effect on LOVO cells.…”

Section: Discussionsupporting

confidence: 92%

Synergistic effect of a combination of nanoparticulate Fe3O4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

Fang

Chen²,

Liu³

et al. 2012

IJN

View full text Add to dashboard Cite

Background: The present study evaluated whether magnetic nanoparticles containing Fe 3 O 4 could enhance the activity of gambogic acid in human colon cancer cells, and explored the potential mechanisms involved. Methods: Cytotoxicity was evaluated by MTT assay. The percentage of cells undergoing apoptosis was analyzed by flow cytometry, and cell morphology was observed under both an optical microscope and a fluorescence microscope. Reverse transcriptase polymerase chain reaction and Western blot assay were performed to determine the transcription of genes and expression of proteins, respectively. Results: Gambogic acid could inhibit proliferation of LOVO cells in a dose-dependent and time-dependent manner and induce apoptosis, which was dramatically enhanced by magnetic nanoparticles containing Fe 3 O 4 . The typical morphological features of apoptosis in LOVO cells were observed after treatment comprising gambogic acid with and without magnetic nanoparticles containing Fe 3 O 4 . Transcription of cytochrome c, caspase 9, and caspase 3 genes was higher in the group treated with magnetic nanoparticles containing Fe 3 O 4 and gambogic acid than in the groups that received gambogic acid or magnetic nanoparticles containing Fe 3 O 4 , but transcription of phosphatidylinositol 3-kinase, Akt, and Bad genes decreased. Notably, expression of cytochrome c, caspase 9, and caspase 3 proteins in the group treated with gambogic acid and magnetic nanoparticles containing Fe 3 O 4 was higher than in the groups receiving magnetic nanoparticles containing Fe 3 O 4 or gambogic acid, while expression of p-PI3K, p-Akt, p-Bad, pro-caspase 9, and pro-caspase 3 degraded. Conclusion: Magnetic nanoparticles containing Fe 3 O 4 can enhance apoptosis induced by gambogic acid which may be closely related to regulation of the PI3K/Akt/Bad pathway in the treatment of human colon cancer.

show abstract

Section: Discussionsupporting

confidence: 92%

Synergistic effect of a combination of nanoparticulate Fe3O4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

Fang

Chen²,

Liu³

et al. 2012

IJN

View full text Add to dashboard Cite

show abstract

“…12 Nanotechnologies and nanoparticles are becoming a focus for human medical application for their unique properties. 13 The diagnostic and therapeutic applications of magnetic iron oxide nanoparticles such as drug delivery, magnetic resonance imaging, hyperthermia techniques, cell separation, and tissue repair have expanded enormously in recent decades. 14 The application of drug-coated polymer nanospheres and nanoparticles to inhibit the related drug resistance has attracted much attention.…”

Section: Introductionmentioning

confidence: 99%

Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells

Xia

Chen²,

Ding³

et al. 2011

IJN

View full text Add to dashboard Cite

This study aims to evaluate the potential benefit of combination therapy of 2-methoxyestradiol (2ME) and magnetic nanoparticles of Fe 3 O 4 (MNPs-Fe 3 O 4 ) on myelodysplastic syndrome (MDS) SKM-1 cells and its underlying mechanisms. The effect of the unique properties of tetraheptylammonium-capped MNPs-Fe 3 O 4 with 2ME on cytotoxicity was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell-cycle distribution and apoptosis were assessed by flow cytometry. The expression of cell-cycle marker protein was measured by Western blotting. Growth inhibition rate of SKM-1 cells treated with the 2ME-loaded MNPs-Fe 3 O 4 was enhanced when compared with 2ME alone. 2ME led to an increase of caspase-3 expression, followed by apoptosis, which was significantly increased when combined with an MNPs-Fe 3 O 4 carrier. Moreover, the copolymer of 2ME with MNPs- Fe 3 O 4 blocked a nearly two-fold increase in SKM-1 cells located in G 2 /M phase than in 2ME alone, which may be associated with an accompanying increase of p21 as well as a decrease in cyclin B1 and cdc2 expression, but there was no obvious difference between the MNPs-Fe 3 O 4 and control group. These findings suggest that the unique properties of MNPs-Fe 3 O 4 as a carrier for 2ME, a new anticancer agent currently in clinical trials, may be a logical strategy to enhance the therapeutic activity of MDS.

show abstract

“…Temperature also has an effect in the stability of the magnetic nanoparticle due to energy transfer from the solvent molecules (Brownian motion) to the nanometric particles. Hence, magnetic nanoparticle can be coated with a biocompatible polymer to enhance its stability [30,31,[48][49][50][51][52][53][54][55][56][57][58][59][60][61][62].…”

Section: Magnetic Nanoparticlementioning

confidence: 99%