Magnetic nanoparticles for nanowarming: seeking a fine balance between heating performance and biocompatibility

Liu, Sangmo; Yin, Yadong

doi:10.1039/d3qm00248a

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…Due to their short lifespan and large demand, research on their long-term storage is of great significance, especially in clinical blood storage, military applications, and public health emergencies . Cryopreservation offers a promising solution for the long-term storage of RBCs . However, during traditional storage processes, RBCs undergo aging and encounter a series of storage lesions.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Cryopreservation Mechanism and Direct Removal Strategy of TAPS in Red Blood Cell Cryopreservation

Zhao,

Liu,

Ekpo

et al. 2024

ACS Biomater. Sci. Eng.

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Cryopreservation of red blood cells (RBCs) plays an indispensable role in modern clinical transfusion therapy. Researchers are dedicated to finding cryoprotectants (CPAs) with high efficiency and low toxicity to prevent RBCs from cryopreservation injury. This study presents, for the first time, the feasibility and underlying mechanisms of a novel CPA called tris(hydroxymethyl)aminomethane-3propanesulfonic acid (TAPS) in RBCs cryopreservation. The results demonstrated that the addition of TAPS achieved a post-thaw recovery of RBCs at 79.12 ± 0.67%, accompanied by excellent biocompatibility (above 97%). Subsequently, the mechanism for preventing RBCs from cryopreservation injury was elucidated. On one hand, TAPS exhibits a significant amount of bound water and effectively inhibits ice recrystallization, thereby reducing mechanical damage. On the other hand, TAPS demonstrates high capacity to scavenge reactive oxygen species and strong endogenous antioxidant enzyme activity, providing effective protection against oxidative damage. Above all, TAPS can be readily removed through direct washing, and the RBCs after washing showed no significant differences in various physiological parameters (SEM, RBC hemolysis, ESR, ATPase activity, and Hb content) compared to fresh RBCs. Finally, the presented mathematical modeling analysis indicates the good benefits of TAPS. In summary, TAPS holds potential for both research and practical in the field of cryobiology, offering innovative insights for the improvement of RBCs cryopreservation in transfusion medicine.

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Section: Introductionmentioning

confidence: 99%

Exploring the Cryopreservation Mechanism and Direct Removal Strategy of TAPS in Red Blood Cell Cryopreservation

Zhao,

Liu,

Ekpo

et al. 2024

ACS Biomater. Sci. Eng.

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“…Magnetic nanoparticles have emerged as a promising platform for nanowarming, a technique that leverages these nanoparticles to generate heat under an alternating magnetic field, thereby facilitating rapid and uniform heating of cryopreserved biological samples. − Their potential to overcome the limitations of traditional convective rewarming methods has been demonstrated across tissues and organs, including rat hearts, livers, and kidneys. − While previous work mainly focused on establishing the fundamental principles, feasibility, and initial protocols for nanowarming, the work used commercially available iron oxide nanoparticle cores and rational optimization of intrinsic nanoparticle properties such as size, assembly states, and surface coating to boost their heating performance remains less investigated. − …”

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confidence: 99%

Engineering Magnetic Nanoclusters for Highly Efficient Heating in Radio-Frequency Nanowarming

Ye,

Tai,

Han

et al. 2024

Nano Lett.

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Effective thawing of cryopreserved samples requires rapid and uniform heating. This is achievable through nanowarming, an approach that heats magnetic nanoparticles by using alternating magnetic fields. Here we demonstrate the synthesis and surface modification of magnetic nanoclusters for efficient nanowarming. Magnetite (Fe 3 O 4 ) nanoclusters with an optimal diameter of 58 nm exhibit a high specific absorption rate of 1499 W/g Fe under an alternating magnetic field at 43 kA/m and 413 kHz, more than twice that of commercial iron oxide cores used in prior nanowarming studies. Surface modification with a permeable resorcinol-formaldehyde resin (RFR) polymer layer significantly enhances their colloidal stability in complex cryoprotective solutions, while maintaining their excellent heating capacity. The Fe 3 O 4 @ RFR nanoparticles achieved a high average heating rate of 175 °C/min in cryopreserved samples at a concentration of 10 mg Fe/mL and were successfully applied in nanowarming porcine iliac arteries, highlighting their potential for enhancing the efficacy of cryopreservation.

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Development of Macromolecular Cryoprotectants for Cryopreservation of Cells

Yuan,

Chen,

Zhu

et al. 2024

Macromol. Rapid Commun.

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Cryopreservation is a common way for long‐term storage of therapeutical proteins, erythrocytes, and mammalian cells. For cryoprotection of these biosamples to keep their structural integrity and biological activities, it is essential to incorporate highly efficient cryoprotectants. Currently, permeable small molecular cryoprotectants such as glycerol and dimethyl sulfoxide dominate in cryostorage applications, but they are harmful to cells and human health. As acting in the extracellular space, membrane‐impermeable macromolecular cryoprotectants, which exert remarkable membrane stabilization against cryo‐injury and are easily removed post‐thaw, are promising candidates with biocompatibility and feasibility. Water‐soluble hydroxyl‐containing polymers such as poly(vinyl alcohol) and polyol‐based polymers are potent ice recrystallization inhibitors, while polyampholytes, polyzwitterions, and bio‐inspired (glyco)polypeptides can significantly increase post‐thaw recovery with reduced membrane damages. In this review, the synthetic macromolecular cryoprotectants are systematically summarized based on their synthesis routes, practical utilities, and cryoprotective mechanisms. It provides a valuable insight in development of highly efficient macromolecular cryoprotectants with valid ice recrystallization inhibition activity for highly efficient and safe cryopreservation of cells.

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Magnetic nanoparticles for nanowarming: seeking a fine balance between heating performance and biocompatibility

Abstract: Regenerative medicine has the potential to revolutionize healthcare by providing transplant options for patients suffering from tissue disease or organ failure. Cryopreservation offers a promising solution for long-term tissue and...

Cited by 4 publications

References 57 publications

Exploring the Cryopreservation Mechanism and Direct Removal Strategy of TAPS in Red Blood Cell Cryopreservation

Exploring the Cryopreservation Mechanism and Direct Removal Strategy of TAPS in Red Blood Cell Cryopreservation

Engineering Magnetic Nanoclusters for Highly Efficient Heating in Radio-Frequency Nanowarming

Development of Macromolecular Cryoprotectants for Cryopreservation of Cells

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