Magnetic nanoparticles for smart electrochemical immunoassays: a review on recent developments

Pastucha, Matěj; Farka, Zdeněk; Lacina, Karel; Mikušová, Zuzana; Skládal, Petr

doi:10.1007/s00604-019-3410-0

Cited by 120 publications

(63 citation statements)

References 128 publications

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“…MBs are a powerful and versatile tool employed in biosensing because they facilitate efficient target analyte retrieval and concentration, minimize matrix effect, reduce largely the assay time and make analytical procedures more compatible with higher sample throughput and automation [23][24][25][26].…”

Section: Electrochemical Affinity Biosensors For the Determination Ofmentioning

confidence: 99%

Cutting-Edge Advances in Electrochemical Affinity Biosensing at Different Molecular Level of Emerging Food Allergens and Adulterants

et al. 2020

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The presence of allergens and adulterants in food, which represents a real threat to sensitized people and a loss of consumer confidence, is one of the main current problems facing society. The detection of allergens and adulterants in food, mainly at the genetic level (characteristic fragments of genes that encode their expression) or at functional level (protein biomarkers) is a complex task due to the natural interference of the matrix and the low concentration at which they are present. Methods for the analysis of allergens are mainly divided into immunological and deoxyribonucleic acid (DNA)-based assays. In recent years, electrochemical affinity biosensors, including immunosensors and biosensors based on synthetic sequences of DNA or ribonucleic acid (RNA), linear, aptameric, peptide or switch-based probes, are gaining special importance in this field because they have proved to be competitive with the methods commonly used in terms of simplicity, test time and applicability in different environments. These unique features make them highly promising analytical tools for routine determination of allergens and food adulterations at the point of care. This review article discusses the most significant trends and developments in electrochemical affinity biosensing in this field over the past two years as well as the challenges and future prospects for this technology.

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Section: Electrochemical Affinity Biosensors For the Determination Ofmentioning

confidence: 99%

Cutting-Edge Advances in Electrochemical Affinity Biosensing at Different Molecular Level of Emerging Food Allergens and Adulterants

et al. 2020

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“…Magnetic particles of different size (nano-and micro-) and various composition resulting in different magnetization (superparamagnetic and ferromagnetic) have found numerous applications in biotechnology [1] and medicine [2][3][4][5]. Particularly, they are used for magneto-controlled targeting (delivering drugs [6,7], genes [8], radiopharmaceuticals [9]), in magnetic resonance imaging [10], in various diagnostic applications [11], for biosensing [12] (e.g., immunoassays [13]), RNA and DNA purification [14], gene cloning, cell separation and purification [15]. Magnetic nano-species with complex topology (e.g., nanorods, nanowires and nanotubes) [16] have been used in numerous nano-technological devices, including tunable micro-fluidic channels with magnetic control [17], data storage units in nano-circuits [18], and magnetized nano-tips for magnetic force microscopes [19].…”

Section: Magnetic Nanoparticles-motivations and Applicationsmentioning

confidence: 99%

Synthesis, Properties and Applications of Magnetic Nanoparticles and Nanowires—A Brief Introduction

Katz

2019

Magnetochemistry

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Magnetic nanoparticles and magnetic nano-species of complex topology (e.g., nanorods, nanowires, nanotubes, etc.) are overviewed briefly in the paper, mostly giving attention to the synthetic details and particle composition (e.g., core-shell structures made of different materials). Some aspects related to applications of magnetic nano-species are briefly discussed. While not being a comprehensive review, the paper offers a large collection of references, particularly useful for newcomers in the research area.

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“…The use of MBs, mainly superparamagnetic, in the development of immunoassays and immunosensors for food analysis and clinical diagnosis is garnering increasing interest [14][15][16], due to the various advantages that the use of MBs can entail, including an increased surface-to-volume ratio, improved assay kinetics, a higher washing efficiency and lower matrix effects. Herein, we describe the exploitation of MBs in different approaches related with the detection of marine toxins, classifying them according to their use as supports, signal amplifiers, capture agents and, finally, for the production of biorecognition molecules.…”

Section: Magnetic Beads In Marine Toxin Detectionmentioning

confidence: 99%

Magnetic Beads in Marine Toxin Detection: A Review

2019

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Due to the expanding occurrence of marine toxins, and their potential impact on human health, there is an increased need for tools for their rapid and efficient detection. We give an overview of the use of magnetic beads (MBs) for the detection of marine toxins in shellfish and fish samples, with an emphasis on their incorporation into electrochemical biosensors. The use of MBs as supports for the immobilization of toxins or antibodies, as signal amplifiers as well as for target pre-concentration, is reviewed. In addition, the exploitation of MBs in Systematic Evolution of Ligands by Exponential enrichment (SELEX) for the selection of aptamers is presented. These MB-based strategies have led to the development of sensitive, simple, reliable and robust analytical systems for the detection of toxins in natural samples, with applicability in seafood safety and human health protection.

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Magnetic nanoparticles for smart electrochemical immunoassays: a review on recent developments

Cited by 120 publications

References 128 publications

Cutting-Edge Advances in Electrochemical Affinity Biosensing at Different Molecular Level of Emerging Food Allergens and Adulterants

Cutting-Edge Advances in Electrochemical Affinity Biosensing at Different Molecular Level of Emerging Food Allergens and Adulterants

Synthesis, Properties and Applications of Magnetic Nanoparticles and Nanowires—A Brief Introduction

Magnetic Beads in Marine Toxin Detection: A Review

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