Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

Liu, Chi-Hsien; Tsao, Min-Han; Sahoo, Soubhagya Laxmi; Wu, Wei‐Chi

doi:10.1039/c6ra25610d

Cited by 14 publications

(8 citation statements)

References 39 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be caused by the DNA binding to bare iron oxide nanoparticles and precipitation of the DNA-SPION complexes by the external magnetic field. Previously the possibility of electrostatic complexes formation between positively charged nanoparticles and DNA was shown in [14][15][16]. The experiments at higher relative DNA concentrations demonstrate qualitatively similar results (Fig.…”

Section: Methodssupporting

confidence: 83%

Interaction of superparamagnetic iron oxide nanoparticles with DNA and BSA

Skuratovska¹,

Pesina²,

Bereznyak³

et al. 2019

Biophysical Bulletin

View full text Add to dashboard Cite

Background: Superparamagnetic iron oxide nanoparticles (SPION) are widely used in various biomedical technologies, in particular, as carriers for drug delivery to the target. Since SPION-drug complexes are planned to be used in vivo, it is necessary to find out if competitive binding of nanoparticles with biologically important macromolecules (nucleic acids and proteins) is possible. Objectives: To investigate the possibility of complexation of iron oxide nanoparticles with DNA and serum albumin. Materials and methods: Bare and sodium citrate coated SPION with different surface charges, bovine serum albumin (BSA) and calf thymus DNA were used. The complexes of SPION and macromolecules were precipitated by an external magnetic field. The research was carried out by spectrophotometry in visible and ultraviolet ranges. Results: To study SPION interactions with DNA and BSA, the spectra of supernatants of the binary systems were compared with the spectra of the corresponding control macromolecules solutions. In the DNA-SPION systems, a decrease in the DNA absorption is observed only for bare nanoparticles. Our estimation shows that the maximum possible concentration ratio of bound DNA to SPION is about 2.5×10-4 mol/g. The addition of sodium citrate coated SPION to the DNA solution does not cause any spectral changes of the supernatant. The interaction of BSA with SPION, coated with sodium citrate, leads to a slight increase in supernatant absorption compared with the one of the control protein solution. It can be caused by the fact that the resulting complexes are not precipitated by a magnetic field. No difference between the spectrum of the supernatant of BSA-bare SPION system and the control protein solution was observed. Conclusions: The obtained spectrophotometric results demonstrate the formation of complexes between DNA and bare iron oxide nanoparticles as well as between BSA and the nanoparticles, coated with sodium citrate. The maximum concentration ratio of bound DNA and bare SPION was obtained for the investigated system. It is necessary to take into account when SPION are used as carriers for drug delivery.

show abstract

Section: Methodssupporting

confidence: 83%

Interaction of superparamagnetic iron oxide nanoparticles with DNA and BSA

Skuratovska¹,

Pesina²,

Bereznyak³

et al. 2019

Biophysical Bulletin

View full text Add to dashboard Cite

show abstract

“…A GFP-fused carbohydrate-binding module (type 3a family [ 41 ], GFP–CBM3A) was selected to detect cellulose in biological tissues and to track its localization in histological slides. The GFP–CBM adsorption on BNC and BCNC was compared and the obtained data fitted to the Langmuir adsorption model [ 43 , 44 ]. Figure 3 shows the adsorption isotherms of GFP–CBM onto BNC and BCNC and the respective parameters of the Langmuir isotherm.…”

Section: Resultsmentioning

confidence: 99%

“…A non-linear regression analysis was used to calculate the parameters of the Langmuir adsorption isotherm [ 43 , 44 ]:

where GFP–CBM Bound is the amount of adsorbed protein per unit mass of cellulose (mg/mg), GFP–CBM Free is the protein concentration (mg/mL) in the liquid phase at the adsorption equilibrium, GFP–CBM Max is the maximum amount of adsorbed protein per unit mass of cellulose (mg/mg), and K a is the Langmuir constant (mL/mg). Three independent assays were performed, each of them in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Tracking Bacterial Nanocellulose in Animal Tissues by Fluorescence Microscopy

et al. 2022

View full text Add to dashboard Cite

The potential of nanomaterials in food technology is nowadays well-established. However, their commercial use requires a careful risk assessment, in particular concerning the fate of nanomaterials in the human body. Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide, has been used as a food product for many years in Asia. However, given its nano-character, several toxicological studies must be performed, according to the European Food Safety Agency’s guidance. Those should especially answer the question of whether nanoparticulate cellulose is absorbed in the gastrointestinal tract. This raises the need to develop a screening technique capable of detecting isolated nanosized particles in biological tissues. Herein, the potential of a cellulose-binding module fused to a green fluorescent protein (GFP–CBM) to detect single bacterial cellulose nanocrystals (BCNC) obtained by acid hydrolysis was assessed. Adsorption studies were performed to characterize the interaction of GFP–CBM with BNC and BCNC. Correlative electron light microscopy was used to demonstrate that isolated BCNC may be detected by fluorescence microscopy. The uptake of BCNC by macrophages was also assessed. Finally, an exploratory 21-day repeated-dose study was performed, wherein Wistar rats were fed daily with BNC. The presence of BNC or BCNC throughout the GIT was observed only in the intestinal lumen, suggesting that cellulose particles were not absorbed. While a more comprehensive toxicological study is necessary, these results strengthen the idea that BNC can be considered a safe food additive.

show abstract

“…Hoechst 33342 has been mostly used for ow cytometry [32][33][34] . On the other hand, H33258 staining has been predominantly used in uorescence microscopy 18,19,35 .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some papers reported that the nucleus of apoptotic cells can exhibit enhanced uorescence after Hoechst binding [15][16][17] . Therefore, Hoechst probes have been frequently used for nucleus staining in uorescence microscopy and ow cytometry [18][19][20] .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Spectrofluorometric Assay Detecting Nuclear Condensation and Fragmentation in Intact Cells

Majtnerová

Čapek

Petira

et al. 2021

Preprint

View full text Add to dashboard Cite

At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy and flow cytometry. However, none of the methods has used the Hoechst probes for quantitative spectrofluorometric assessment. Therefore, the aim of present study was to develop a spectrofluorometric assay for detection of nuclear condensation and fragmentation in intact cells. We used HepG2 and HK‑2 cells cultured in 96-well plates which were treated with potent apoptotic inducers (i.e. cisplatin, staurosporine, camptothecin) for 6-48 h. Then, the cells were incubated with Hoechst 33258 (2 µg/mL) and the increase of fluorescence after binding of the dye to DNA was measured. The developed spectrofluorometric assay was capable to detect nuclear changes caused by all tested apoptotic inducers. Then, we compared the outcomes of the spectrofluorometric assay with other methods detecting apoptosis (i.e. TUNEL, DNA ladder, caspase activity). We found that the developed assay provides results of same sensitivity as TUNEL assay but the advantages of the spectrofluorometric assay are fast processing, low-cost and high throughput. Because nuclear condensation and fragmentation can be typical markers of cell death, especially in apoptosis, we suppose that the spectrofluorometric assay could become a routinely used method for characterizing cell death processes.

show abstract

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

Abstract: The fluorescence magnetic nanoparticles offer versatile platforms for nucleus imaging and DNA adsorption.

Cited by 14 publications

References 39 publications

Interaction of superparamagnetic iron oxide nanoparticles with DNA and BSA

Interaction of superparamagnetic iron oxide nanoparticles with DNA and BSA

Tracking Bacterial Nanocellulose in Animal Tissues by Fluorescence Microscopy

Quantitative Spectrofluorometric Assay Detecting Nuclear Condensation and Fragmentation in Intact Cells

Contact Info

Product

Resources

About