Magnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells

Novoselova, Iuliia P.; Neusch, Andreas; Brand, Julia-Sarita; Otten, Marius; Safari, Mohammad Reza; Bartels, Nina; Karg, Matthias; Farle, Michael; Wiedwald, Ulf; Monzel, Cornelia

doi:10.3390/nano11092267

Cited by 6 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The magnetic cores of BNF comprise several crystallites of magnetic iron oxide having a nominal diameter of 92 ± 19 nm. Both are comparable with previously reported sizes [35,36]. Magnetosomes preserved within chains exhibited an average diameter of (39 ± 7) nm, not accounting for the protein rich membrane, which naturally surrounds the magnetosomes regulating the crystal maturation [37].…”

Section: Characterization Of Mnp Physical Propertiessupporting

confidence: 89%

An efficient magnetothermal actuation setup for fast heating/cooling cycles or long-term induction heating of different magnetic nanoparticle classes

Kuckla,

Brand,

Czech

et al. 2023

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

Alternating magnetic fields (AMFs) in the ~100 kHz frequency regime cause magnetic nanoparticles (MNPs) to dissipate heat to their nanoscale environment. This mechanism is beneficial for a variety of applications in biomedicine and nanotechnology, such as localized heating of cancer tissue, actuation of drug release, or inducing conformational changes of molecules. However, engineering electromagnetic resonant circuits which generate fields to efficiently heat MNPs over long time scales, remains a challenge. In addition, many applications require fast heating/cooling cycles over ΔT = 5-10 °C to switch the sample between different states. Here, we present a home-built magnetothermal actuation setup maximized in its efficiency to deliver stable AMFs as well as to enable fast heating/cooling cycles of MNP samples. The setup satisfies various demands, such as an elaborate cooling system to control heating of the circuit components as well as of the sample due to inductive losses. Fast cycles of remote sample heating/cooling (up to ±15 °C/min) as well as long-term induction heating were monitored via contact-free thermal image recording at sub-mm resolution. Next to characterizing the improved hyperthermia setup, we demonstrate its applicability to heat different types of MNPs: ‘Nanoflower’-shaped multicore iron oxide nanoparticles, core shell magnetite MNPs, as well as Magnetosomes from magnetotactic bacteria (Magnetospirillum gryphiswaldense). MNPs are directly compared in their structure, surface charge, magnetic properties as well as heating response. Our work provides practical guidelines for AMF engineering and the monitoring of MNP heating for biomedical or nano-/biotechnological applications.

show abstract

Section: Characterization Of Mnp Physical Propertiessupporting

confidence: 89%

An efficient magnetothermal actuation setup for fast heating/cooling cycles or long-term induction heating of different magnetic nanoparticle classes

Kuckla,

Brand,

Czech

et al. 2023

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The significant advantage of this model's material selection is the quasi-spheroidal shape suitable for mathematical simulation and theory formulations [6]. The modern trend of the last few decades is searching for magnetite (Fe 3 O 4 )-based materials, which should simultaneously meet high stability and biocompatibility for biomedical applications [7,8]. The greatest challenge is overcoming the two common problems during such material preparation:…”

Section: Introductionmentioning

confidence: 99%

Variations in the Structural and Colloidal Stability of Magnetoferritin under the Impact of Technological Process Modulations

Balejcikova,

Zolochevska,

Tomasovicova

et al. 2023

Crystals

View full text Add to dashboard Cite

Iron-based materials, especially magnetite nanocrystals, have found extensive applications in many fields. Novel challenges focus on a deeper understanding of interactions between magnetite and biological macromolecules for developing further applications in diagnostic and treatment methods in medicine. Inspired by ferritin, the iron storage protein occurring in bacteria, plant, animal, and human cells, we developed an artificial ferritin-like material known as magnetoferritin. We present structural studies of magnetoferritin samples prepared using a controlled in vitro physicochemical synthesis. Considerable structural and size changes were observed by increasing the iron content and post-synthesis treatment. We propose the modulation of colloidal stability by using suitable solvents. Ultraviolet and visible spectroscopy, dynamic light scattering, colloidal stability measurements, infrared spectroscopy, and small-angle X-ray scattering methods were employed. The presented results aid in increasing the effectiveness of the various applications of magnetoferritin according to specific industrial requirements.

show abstract

“…Magnetic nanoparticles, being structures whose dimensions lie in the nanoscale, constitute an excellent tool for such meticulously defined precision. Moreover, their magnetic properties allow for the remote, controlled, and non-invasive application of the defined mechanical forces at the magnitude, direction, and application time required [10,11].…”

Section: Introductionmentioning

confidence: 99%

Magnetomechanical Stress-Induced Colon Cancer Cell Growth Inhibition

Spyridopoulou

Aindelis

Sarafidis

et al. 2022

JNT

View full text Add to dashboard Cite

The application of magnetomechanical stress in cells using internalized magnetic nanoparticles (MNPs) actuated by low-frequency magnetic fields has been attracting considerable interest in the field of cancer research. Recent developments prove that magnetomechanical stress can inhibit cancer cells’ growth. However, the MNPs’ type and the magnetic field’s characteristics are crucial parameters. Their variability allows multiple combinations, which induce specific biological effects. We previously reported the antiproliferative effects induced in HT29 colon cancer cells by static-magnetic-field (200 mT)-actuated spherical MNPs (100 nm). Herein, we show that similar growth inhibitory effects are induced in other colon cancer cell lines. The effect of magnetomechanical stress was also examined in the growth rate of tumor spheroids. Moreover, we examined the biological mechanisms involved in the observed cell growth inhibition. Under the experimental conditions employed, no cell death was detected by PI (propidium iodide) staining analysis. Flow cytometry and Western blotting revealed that G2/M cell cycle arrest might mediate the antiproliferative effects. Furthermore, MNPs were found to locate in the lysosomes, and a decreased number of lysosomes was detected in cells that had undergone magnetomechanical stress, implying that the mechanical activation of the internalized MNPs could induce lysosome membrane disruption. Of note, the lysosomal acidic conditions were proven to affect the MNPs’ magnetic properties, evidenced by vibrating sample magnetometry (VSM) analysis. Further research on the combination of the described magnetomechanical stress with lysosome-targeting chemotherapeutic drugs could lay the groundwork for the development of novel anticancer combination treatment schemes.

show abstract

Magnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells

Cited by 6 publications

References 56 publications

An efficient magnetothermal actuation setup for fast heating/cooling cycles or long-term induction heating of different magnetic nanoparticle classes

An efficient magnetothermal actuation setup for fast heating/cooling cycles or long-term induction heating of different magnetic nanoparticle classes

Variations in the Structural and Colloidal Stability of Magnetoferritin under the Impact of Technological Process Modulations

Magnetomechanical Stress-Induced Colon Cancer Cell Growth Inhibition

Contact Info

Product

Resources

About