Magnetic particle detection by frequency mixing for immunoassay applications

Krause, Hans‐Joachim; Wolters, N.; Zhang, Yi; Offenhäusser, Andreas; Miethe, Peter; Meyer, M Cobas; Hartmann, Markus; Keusgen, Michael

doi:10.1016/j.jmmm.2006.10.1164

Cited by 151 publications

(151 citation statements)

References 20 publications

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“…The magnetic particles were detected by frequency mixing as described previously (34,35). The magnetic reader is a small handheld device with an intuitive touch screen allowing parameter adjustment and measurement control (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Magnetic saturation of the superparamagnetic particles was achieved at each positive and negative extreme of the applied driving field, which alternated with a frequency of 2f 2 of 122 Hz (34,35).…”

Section: Methodsmentioning

confidence: 99%

“…The voltage induced in the detection coil was preamplified, and subsequently, the mixing component (f 1 ϩ 2f 2 ) was demodulated by twostage multiplication and low-pass filtering, enabling signal-dependent calibration using serially diluted samples with iron oxide concentrations of 0.12 to 1,300 mg/liter (34).…”

Section: Methodsmentioning

confidence: 99%

“…The nanoparticles enter a magnetic field comprising two different excitation frequencies, and the response signal (in mV) is detected at a third mixing frequency, allowing quantification over a large dynamic range of more than 4 orders of magnitude (34).…”

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

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Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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c Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 g/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus, with detection limits of 2 to 60 ng/ml.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

See 2 more Smart Citations

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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“…To exploit this nonlinearity, we use the frequency mixing method in order to detect the presence of particles in a microfluidic channel. In this method, two magnetic excitation signals are applied to the sample reservoir at distinct frequencies (f1, f2) and the amplitude of the response signal is measured at a specific frequency ( + 2 ) that allows detecting and quantifying the amount of SPN [9]. The magnetic frequency mixing technique is very well suited for this purpose, thus allowing its use for immunoquantification.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Detection Structure for LOC Immunoassays, Multiphysics Simulations and Experimental Results

Rabehi¹,

Garlan²,

Shanehsazzadeh

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Abstract:The aim of this work is to develop a completely integrated Lab-On-Chip (LOC) for easy, rapid and cost-effective immunoassays. The pathogen sensing system is composed of a microfluidic channel surrounded by planar microcoils which are responsible for the emission and the detection of magnetic fields. The system allows the detection and quantification of superparamagnetic beads used for immunoassays in a "sandwich" antigen-antibody configuration. Multiphysics simulations have been achieved and preliminary experimental results have allowed to validate the structure.

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Effect of Polymer and Cell Membrane Coatings on Theranostic Applications of Nanoparticles: A Review

Rezaei,

Harun,

et al. 2024

Adv Healthcare Materials

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The recent decade has witnessed a remarkable surge in the field of nanoparticles, from their synthesis, characterization, and functionalization to diverse applications. At the nanoscale, these particles exhibit distinct physicochemical properties compared to their bulk counterparts, enabling a multitude of applications spanning energy, catalysis, environmental remediation, biomedicine, and beyond. This review focuses on specific nanoparticle categories, including magnetic, gold, silver, and quantum dots (QDs), as well as hybrid variants, specifically tailored for biomedical applications. A comprehensive review and comparison of prevalent chemical, physical, and biological synthesis methods are presented. To enhance biocompatibility and colloidal stability, and facilitate surface modification and cargo/agent loading, nanoparticle surfaces are coated with different synthetic polymers and very recently, cell membrane coatings. The utilization of polymer‐ or cell membrane‐coated nanoparticles opens a wide variety of biomedical applications such as magnetic resonance imaging (MRI), hyperthermia, photothermia, sample enrichment, bioassays, drug delivery, etc. With this review, the goal is to provide a comprehensive toolbox of insights into polymer or cell membrane‐coated nanoparticles and their biomedical applications, while also addressing the challenges involved in translating such nanoparticles from laboratory benchtops to in vitro and in vivo applications. Furthermore, perspectives on future trends and developments in this rapidly evolving domain are provided.

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Magnetic particle detection by frequency mixing for immunoassay applications

Cited by 151 publications

References 20 publications

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Magnetic Detection Structure for LOC Immunoassays, Multiphysics Simulations and Experimental Results

Effect of Polymer and Cell Membrane Coatings on Theranostic Applications of Nanoparticles: A Review

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