Magnetic Polymer Microcarriers for Cell Engineering Applications with Enzyme‐Free Cell Harvesting and Rapid Recovery in Large‐Scale Cell Culture

Liu, Cong; Meng, Meng; Yang, Zhiyu; Wu, Shaobin; Shang, Ludan; Feng, Yuanji; Wu, Jiayan; Hao, Kai; Wang, Ruonan; Zhou, Danhua; He, Shasha; Li, Yanhui; Tian, Huayu

doi:10.1002/adfm.202311454

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…The enhanced electronegativity of carbonyl oxygen attracts Na + toward itself, increasing the electron cloud density of the Si−O bond away from Na + , reducing the binding energy from 531.19 to 530.68 eV. 44 These results certify a wide-temperature (30−70 °C) phase transition hydrogel, offering a platform for mechanical and electrical properties and functional sensing implementation. 45,46 Increasing the XLG content enhances PNX's mechanical attributes (Figure 2a).…”

mentioning

confidence: 74%

Self-Adhesive, Stretchable, and Thermosensitive Iontronic Hydrogels for Highly Sensitive Neuromorphic Sensing–Synaptic Systems

Chen,

Zhou

et al. 2024

Nano Lett.

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Artificial sensory afferent nerves that emulate receptor nanochannel perception and synaptic ionic information processing in chemical environments are highly desirable for bioelectronics. However, challenges persist in achieving life-like nanoscale conformal contact, agile multimodal sensing response, and synaptic feedback with ions. Here, a precisely tuned phase transition poly(N-isopropylacrylamide) (PNIPAM) hydrogel is introduced through the water molecule reservoir strategy. The resulting hydrogel with strongly cross-linked networks exhibits excellent mechanical performance (∼2000% elongation) and robust adhesive strength. Importantly, the hydrogel’s enhanced ionic conductance and heterogeneous structure of the temperature-sensitive component enable highly sensitive strain information perception (GFmax = 7.94, response time ∼ 87 ms), temperature information perception (TCRmax = −1.974%/°C, response time ∼ 270 ms), and low energy consumption synaptic plasticity (42.2 fJ/spike). As a demonstration, a neuromorphic sensing–synaptic system is constructed integrating iontronic strain/temperature sensors with fiber synapses for real-time information sensing, discrimination, and feedback. This work holds enormous potential in bioinspired robotics and bioelectronics.

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confidence: 74%

Self-Adhesive, Stretchable, and Thermosensitive Iontronic Hydrogels for Highly Sensitive Neuromorphic Sensing–Synaptic Systems

Chen,

Zhou

et al. 2024

Nano Lett.

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“…Magnetic polymer composite microspheres (MPCMs) have garnered significant attention for applications in targeted drug delivery, , immunoassays, environmental pollutant detection, and biologic separation , due to the integration of the multifunctionality of polymer materials with the magnetic responsiveness and good biocompatibility of inorganic magnetic materials. However, research on MPCMs has been predominantly focused on large (10–100 μm) and nanoscale sizes, with scant reports addressing cell-level (1–10 μm) MPCMs with high magnetization loading.…”

Section: Introductionmentioning

confidence: 99%

Carboxyl-Assisted Coordination-Driven Self-Assembly for the Preparation of Magnetic Polymer Composite Microspheres

Ren,

Yan,

et al. 2024

ACS Appl. Polym. Mater.

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Magnetic polymer composite microspheres (MPCMs) with biocompatible coatings are increasingly being recognized as promising candidates for biologic separation, immunoassays, and drug delivery systems. Notwithstanding, conventional preparation methods primarily involve the simple stacking or disordered assembly of magnetic particles. In this study, carboxylate ligands of the poly(styrene-alt-maleic acid) sodium salt (PSMA-Na) facilitate the self-assembly preparation of PSDA@Fe 3 O 4 composite microspheres through bidentate bridging coordination to surface metal ions of Fe 3 O 4 . The critical role of carboxyl groups within the amphiphilic alternating copolymer PSMA-Na can be determined so that (1) these can help to establish strong coordination bonds with Fe 3 O 4 , serving as a bridge between the magnetic nanoparticles and the polymer material, thereby facilitating the capture of magnetic components; (2) more intriguingly, the magnetic materials within this system undergo a self-assembly process transitioning from the core to the shell, where the strong coordination effect of carboxyl groups results in the formation of a core−shell structure with Fe 3 O 4 uniformly dispersed on the surface of polymer microspheres. Consequently, the PSDA@Fe 3 O 4 microspheres produced exhibit controllable particle size, high magnetic loading, and excellent biocompatibility, presenting potential biomaterial applications in magnetic resonance imaging and drug delivery systems. Significantly, this self-assembly method is straightforward, swift, and widely applicable to nanoparticles and carboxylate organic molecules, offering insights and inspiration for synthesizing additional composite materials.

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Engineering Thermoresponsive Poly(N-isopropylacrylamide)-Based Films with Enhanced Stability and Reusability for Efficient Bone Marrow Mesenchymal Stem Cell Culture and Harvesting

Yang,

Sun,

Sun

et al. 2024

Molecules

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Poly(N-isopropylacrylamide) (PNIPAM) offers a promising platform for non-invasive and gentle cell detachment. However, conventional PNIPAM-based substrates often suffer from limitations including limited stability and reduced reusability, which hinder their widespread adoption in biomedical applications. In this study, PNIPAM copolymer films were formed on the surfaces of glass slides or silicon wafers using a two-step film-forming method involving coating and grafting. Subsequently, a comprehensive analysis of the films’ surface wettability, topography, and thickness was conducted using a variety of techniques, including contact angle analysis, atomic force microscopy (AFM), and ellipsometric measurements. Bone marrow mesenchymal stem cells (BMMSCs) were then seeded onto PNIPAM copolymer films prepared from different copolymer solution concentrations, ranging from 0.2 to 10 mg·mL−1, to select the optimal culture substrate that allowed for good cell growth at 37 °C and effective cell detachment through temperature reduction. Furthermore, the stability and reusability of the optimal copolymer films were assessed. Finally, AFM and X-ray photoelectron spectroscopy (XPS) were employed to examine the surface morphology and elemental composition of the copolymer films after two rounds of BMMSC adhesion and detachment. The findings revealed that the surface properties and overall characteristics of PNIPAM copolymer films varied significantly with the solution concentration. Based on the selection criteria, the copolymer films derived from 1 mg·mL−1 solution were identified as the optimal culture substrates for BMMSCs. After two rounds of cellular adhesion and detachment, some proteins remained on the film surfaces, acting as a foundation for subsequent cellular re-adhesion and growth, thereby implicitly corroborating the practicability and reusability of the copolymer films. This study not only introduces a stable and efficient platform for stem cell culture and harvesting but also represents a significant advance in the fabrication of smart materials tailored for biomedical applications.

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Magnetic Polymer Microcarriers for Cell Engineering Applications with Enzyme‐Free Cell Harvesting and Rapid Recovery in Large‐Scale Cell Culture

Cited by 3 publications

References 43 publications

Self-Adhesive, Stretchable, and Thermosensitive Iontronic Hydrogels for Highly Sensitive Neuromorphic Sensing–Synaptic Systems

Self-Adhesive, Stretchable, and Thermosensitive Iontronic Hydrogels for Highly Sensitive Neuromorphic Sensing–Synaptic Systems

Carboxyl-Assisted Coordination-Driven Self-Assembly for the Preparation of Magnetic Polymer Composite Microspheres

Engineering Thermoresponsive Poly(N-isopropylacrylamide)-Based Films with Enhanced Stability and Reusability for Efficient Bone Marrow Mesenchymal Stem Cell Culture and Harvesting

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