Magnetic polymeric nanoparticles functionalized by mannose‐rhodamine conjugate for detection of E. coli

Abstract

Mannose-rhodamine (Rh) conjugate (80% yield) was synthesized in a one-pot reaction and immobilized onto magnetic polymeric nanoparticles (MPNP; 43% magnetic content) of poly(styrene/divinyl benzene/acrylic acid). The resulting nanoparticles contained MPNP as a substrate, mannose as an E. coli receptor and Rh as a fluorescent signaling unit. TEM imaging clearly demonstrated that multiple mannose-Rh MPNPs could be captured by E. coli strain ORN178. The fluorescent signal from captured nanoparticles was emitted at 580 nm. These results indicate that mannose-Rh MPNP offers a simple and rapid strategy for bacterial detection.

Tetraphenylethylene-Based AIE-Active Probes for Sensing Applications

“…One of the most common bacterial infections affecting humans, which causes intestinal and extra-intestinal problems such as diarrhea, food poisoning, and urinary tract infections, is due to Escherichia coli. 29 Therefore, early-stage treatment for these infections would be beneficial to arrest the progression of the diseases. Traditional urine pathogenic bacteria culture analysis suffers from various drawbacks such as low sensitivity and a time-consuming procedure with tedious steps, which means it is not a valid approach for the rapid screening of susceptible patients.…”

“…Infectious diseases pose a significant challenge to human health due to bacterial contamination of food, water, and air. One of the most common bacterial infections affecting humans, which causes intestinal and extra-intestinal problems such as diarrhea, food poisoning, and urinary tract infections, is due to Escherichia coli . Therefore, early-stage treatment for these infections would be beneficial to arrest the progression of the diseases.…”

Electrospun Fibrous Mats with Conjugated Tetraphenylethylene and Mannose for Sensitive Turn-On Fluorescent Sensing of Escherichia coli

“…Xue et al incubated E. coli with a mannose-bearing glycopolymer, resulting in the formation of fluorescent cell clusters and significant red shifts in ultraviolet absorption and fluorescent spectra of the polymer . In addition, the weakness of mannose–FimH interactions requires the attachment of a single E. coli cell to several mannose molecules for an efficient complex formation . In the current study, the conjugate of TPE and mannose (TPE-Man) was grafted on electrospun fibers for detection of E. coli in aqueous solution, taking advantage of the high grafting capabilities of ultrafine fibers and the highly porous structure of the fibrous mat to entrap bacterial cells.…”

“…Escherichia coli ( E. coli ) is frequently associated with a number of infectious diseases, causing various intestinal or extraintestinal infections, such as diarrhea, food poisoning, and urinary tract infections . The presence of E. coli is currently considered as an indicator of microbiological quality deterioration, and an accurate routine testing is crucial for the outbreak prevention.…”

“…18 Escherichia coli (E. coli) is frequently associated with a number of infectious diseases, causing various intestinal or extraintestinal infections, such as diarrhea, food poisoning, and urinary tract infections. 19 The presence of E. coli is currently considered as an indicator of microbiological quality deterioration, and an accurate routine testing is crucial for the outbreak prevention. Like most other Gram-negative pathogens, E. coli establishes infections after adhering to host tissues through interactions between bacterial fimbria and mannose moieties from the host cell surface.…”