have been extensively studied in the last decade, both in the fields of fundamental science and applications to spintronics devices. [1,2] One of the well-known mechanisms of skyrmion formation is the competition between ferromagnetic exchange interaction and the Dzyaloshinskii-Moriya interaction (DMI) arising from the lack of inversion symmetry. Skyrmions with helical (Bloch type) and cycloidal (Néel type) spin configurations have been observed in non-centrosymmetric magnets with chiral (T or O class) and polar (C nv class) structures, respectively. [3][4][5][6][7][8][9] Recently, a new topological spin texture, the antiskyrmion, with opposite sign of N sk for the same polarity has attracted much attention. The antiskyrmion consists of both Bloch and Néel walls with the opposite helicities along two orthogonal axes, and its formation is attributed to the anisotropic DMI present in non-centrosymmetric tetragonal crystals belonging to the D 2d or S 4 symmetry group both containing fourfold rotoinversion (4). [10,11] In real materials, antiskyrmions were first found in Heusler compounds with D 2d symmetry, Mn 1.4 PtSn and Mn 1.4 Pt 0.9 Pd 0.1 Sn. [12,13] More recently, the formation of antiskyrmions was also found in Fe 1.9 Ni 0.9 Pd 0.2 P [Pd-doped (Fe,Ni) 3 P] with S 4 symmetry [14] and Fe/Gd-based multilayers. [15] Lorentz transmission electron microscopy (LTEM) observation for thin plates of these Magnetic skyrmions, vortex-like topological spin textures, have attracted much interest in a wide range of research fields from fundamental physics to spintronics applications. Recently, growing attention is also paid to antiskyrmions emerging with opposite topological charge in non-centrosymmetric magnets with D 2d or S 4 symmetry. In these magnets, complex interplay among anisotropic Dzyaloshinskii-Moriya interaction, uniaxial magnetic anisotropy, and magnetic dipolar interactions generates various magnetic textures. However, the precise role of these magnetic interactions in stabilizing antiskyrmions remains to be elucidated. In this work, the uniaxial magnetic anisotropy of schreibersite (Fe,Ni) 3 P with S 4 symmetry is controlled by doping and its impact on the stability of antiskyrmions is investigated. The authors' magnetometry study, supported by ferromagnetic resonance spectroscopy, shows that the variation of the Ni content and slight doping with 4d transition metals considerably change the magnetic anisotropy. In particular, doping with Pd induces easy-axis anisotropy, giving rise to formation of antiskyrmions, while a temperature-induced spin reorientation is observed in an Rh-doped compound. In combination with Lorentz transmission electron microscopy and micromagnetic simulations, the stability of antiskyrmion as functions of uniaxial anisotropy and demagnetization energy is quantitatively analyzed, and demonstrated that subtle balance between them is necessary to stabilize the antiskyrmions.