Magnetic Recovery of Cellulase from Cellulose Substrates with Bare Iron Oxide Nanoparticles

Schnell, Florian; Kube, Massimo; Berensmeier, Sonja; Schwaminger, Sebastian P.

doi:10.1002/cnma.201800658

Cited by 14 publications

(20 citation statements)

References 40 publications

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“…Figure 1 shows images of the particles taken via TEM. The frequency distributions are in good agreement with previous measurements of magnetite particles [15,21,[25][26][27]. Here, the actual dimensions of the individual particles can be determined optically, whereas dynamic light scattering yields the hydrodynamic diameter of agglomerates [17].…”

Section: Resultssupporting

confidence: 89%

“…Bare iron oxide nanoparticles (BIONs) with 5-50 nm size can be used for e.g. protein purification [13,14], enzyme immobilization [15] and harvesting of algae [16]. Usually, the nanoparticles are embedded in polymers and used as superparamagnetic microbeads (0.1-100 µm) and are further modified with functional group to anchor an immunoactive group, such as an antibody [17].…”

Section: Introductionmentioning

confidence: 99%

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Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles

et al. 2020

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The early detection of Legionella in water reservoirs, and the prevention of their often fatal diseases, requires the development of rapid and reliable detection processes. A method for the magnetic separation (MS) of Legionella pneumophila by superparamagnetic iron oxide nanoparticles is developed, which represents the basis for future bacteria detection kits. The focus lies on the separation process and the simplicity of using magnetic nanomaterials. Iron oxide nanoparticles are functionalized with epoxy groups and Legionella-specific antibodies are immobilized. The resulting complexes are characterized with infrared spectroscopy and tested for the specific separation and enrichment of the selected microorganisms. The cell-particle complexes can be isolated in a magnetic field and detected with conventional methods such as fluorescence detection. A nonspecific enrichment of bacteria is also possible by using bare iron oxide nanoparticles (BIONs), which we used as a reference to the nanoparticles with immobilized antibodies. Furthermore, the immunomagnetic separation can be applied for the detection of multiple other microorganisms and thus might pave the way for simpler bacterial diagnosis.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles

et al. 2020

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“…With an average diameter of 9.93 nm, the results are comparable to previous measurements of BIONs [ 32 ]. The particle size distribution of BIONs synthesized by co-precipitation lies between 6 and 14 nm [ 32 , 33 ]. In addition to TEM measurements, the particle size was determined with XRD analysis.…”

Section: Resultsmentioning

confidence: 99%

“…This significantly influences the ionic interaction with the peptide LL to the BION surface (Figure 4e). The five lysine groups that generate a In addition to the particle size, the crystal structure composition and the particles' magnetic behavior play an important role in developing a magnetic drug delivery system as these parameters influence the controllability by an external magnetic field [33,34]. Examination of the crystal structure with XRD analysis shows the typical reflections ((220), (311), (400), (511), and (440)) for magnetite with its cubic structure (Figure 3a) [35].…”

Section: Performance Of Bions As Carrier Materials For Antimicrobial Peptidesmentioning

confidence: 99%

Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin

2021

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New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on Escherichia coli show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.

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“…With these magnetic properties, iron oxide nanoparticles are the basis for multiple applications such as magnetic imaging, magnetic targeting and hyperthermia [4,5]. Aside from medical applications, iron oxide nanoparticles can be used in protein purification [6,7], enzyme and catalyst immobilization [8][9][10], sensing [11] and energy storage [12,13].…”

Section: Introductionmentioning

confidence: 99%

Controlled Synthesis of Magnetic Iron Oxide Nanoparticles: Magnetite or Maghemite?

2020

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Today, magnetic nanoparticles are present in multiple medical and industrial applications. We take a closer look at the synthesis of magnetic iron oxide nanoparticles through the co-precipitation of iron salts in an alkaline environment. The variation of the synthesis parameters (ion concentration, temperature, stirring rate, reaction time and dosing rate) change the structure and diameter of the nanoparticles. Magnetic iron oxide nanoparticles are characterized by X-ray diffraction (XRD), Raman spectroscopy and transmission electron microscopy (TEM). Magnetic nanoparticles ranging from 5 to 16 nm in diameter were synthesized and their chemical structure was identified. Due to the evaluation of Raman spectra, TEM and XRD, the magnetite and maghemite nanoparticles can be observed and the proportion of phases and the particle size can be related to the synthesis conditions. We want to highlight the use of Raman active modes A 1g of spinel structured iron oxides to determine the content of magnetite and maghemite in our samples. Magnetite nanoparticles can be derived from highly alkaline conditions even without establishing an inert atmosphere during the synthesis. The correlation between the particle properties and the various parameters of the synthesis was modelled with linear mixture models. The two models can predict the particle size and the oxidation state of the synthesized nanoparticles, respectively. The modeling of synthesis parameters not only helps to improve synthesis conditions for iron oxide nanoparticles but to understand crystallization of nanomaterials.Crystals 2020, 10, 214 2 of 12 For these applications, an understanding and development of effective and low-cost synthesis routes are necessary. The size, stability and composition of magnetic nanoparticles need to be controlled in order to affect magnetic, optic and surface properties [5,14]. Since these physical and chemical properties are often related to the dimension of nanomaterials, the most simple approach is to investigate the size of nanoparticles for distinct synthesis conditions [2,[15][16][17]. Forge et al. and Roth et al. investigated the influence of several parameters on the particle size of iron oxide nanoparticles based on the alkaline co-precipitation synthesis [17][18][19]. Other studies focus on the formation pathways of iron oxide nanoparticles depending on the synthesis conditions and especially on the reaction time [20]. Such studies include the formation, which can be quite versatile for multiple synthesis routes [2,14,[20][21][22][23][24][25][26]. Although some works approach parameter studies by design of experiments, multiple questions concerning the co-precipitation are not understood [17,18]. For these nanoscale particles, it is quite challenging to characterize and predict their structure, especially at the surface [14,27]. Multiple approaches exist on classifying magnetic iron oxide nanoparticles and describing them according to their properties, which are strongly dependent on their environment, synthesis conditions...

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Magnetic Recovery of Cellulase from Cellulose Substrates with Bare Iron Oxide Nanoparticles

Cited by 14 publications

References 40 publications

Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles

Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles

Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin

Controlled Synthesis of Magnetic Iron Oxide Nanoparticles: Magnetite or Maghemite?

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