Magnetic Resonance Imaging, Microangiography, and Histology in a Rat Model of Primary Liver Cancer

Ni, Yicheng; Marchal, Guy; Damme, Baudouin van; Hecke, Paul Van; Michiels, Johan; Zhang, Xiaowei; Yu, Jie; Baert, Albert

doi:10.1097/00004424-199209000-00006

Cited by 38 publications

(33 citation statements)

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“…The uptake and elimination of gadoxetic acid are believed to require a higher level of hepatic function that involves the organic anion transport system such as the sodium-independent organic anion-transporting polypeptide (OATP1) and biliary drainage system by means of the adenosine triphosphate (ATP)-dependent canalicular multispecific organic anion transporter peptide (cMOAT), in which gadoxetic acid competes with bilirubin for the same organic anion excretory mechanisms (14)(15)(16)(17). It is known that positive contrast enhancement of HCCs on gadoxetic acid-enhanced hepatobiliary-phase images can be theoretically attributed to both active uptake of gadoxetic acid by HCCs with a residual hepatocytic function and impaired biliary excretion within the intratumoral region, as has been demonstrated in animal studies (12,15,18,19). Therefore, it seems that only highly differentiated HCCs and benign nodules can possibly preserve this hepatocytic function.…”

Section: Discussionmentioning

confidence: 99%

Comparison between areas with Gd‐EOB‐DTPA uptake and without in hepatocellular carcinomas on Gd‐EOB‐DTPA‐enhanced hepatobiliary‐phase MR imaging: Pathological correlation

Lee

Kim

Park

et al. 2010

Magnetic Resonance Imaging

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Purpose: To determine histological and MR imaging differences between areas with Gd-EOB-DTPA (gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid) uptake and without in hepatocellular carcinomas (HCCs) as seen on Gd-EOB-DTPA-enhanced hepatobiliary-phase MR images. Materials and Methods:This study included nine patients with nine histopathologically proven HCCs (mean size, 1.9 cm) that consisted of two portions of a non-hypointense (Gd-EOB-DTPA uptake) and hypointense area (no Gd-EOB-DTPA uptake) in one tumor as depicted on hepatobiliary-phase MR images. Two radiologists and one pathologist compared the histological and MR finding differences between the two portions in consensus.Results: In eight specimens, non-hypointense areas of six specimens showed a green color and two specimens did not show a green color. Microscopically, two of nine showed a higher percentage of bile pigments in the nonhypointense area as compared to the hypointense area and the remaining seven showed no difference. Six were homogeneous Edmondson-Steiner grade II, and one was grade I. In two, non-hypointense areas were grade II and hypointense areas were grade I. No difference between the two portions was found for necrosis, hemorrhage, fibrosis, and a fibrous capsule. Seven on T1/T2-weighted images and eight on arterial and portal phase images showed no different signal intensity between the two portions. Conclusion:Although macroscopically, a non-hypointense area of an HCC seen on GD-EOB-DTPA-enhanced hepatobiliary-phase images may be associated with the area with a green color, no definite microscopic and MR imaging findings that could discriminate a non-hypointense from a hypointense area of an HCC was found.

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Section: Discussionmentioning

confidence: 99%

Comparison between areas with Gd‐EOB‐DTPA uptake and without in hepatocellular carcinomas on Gd‐EOB‐DTPA‐enhanced hepatobiliary‐phase MR imaging: Pathological correlation

Lee

Kim

Park

et al. 2010

Magnetic Resonance Imaging

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“…[3H]BaP adducts were also lower in the tumors than the surrounding tissue in both groups, although the DCA group has quantitatively more adducts in both the tumor and surrounding DNA (Fig. 8b) (10,19,20,32). We obtained images with high spatial resolution and contrast using both T, -and T2-weighted spin-echo sequences.…”

Section: Introductionmentioning

confidence: 91%

“…No attempt was made in this study to correlate histologic tumor type to image characteristics. Studies using various contrast agents (e.g., gadolinium conjugates, manganese dipyridoxal-diphosphate, superparamagnetic iron oxides) have allowed some degree of differentiation of tumor type based on vascularity and cellular uptake and excretion characteristics (21,31,32).…”

Section: Introductionmentioning

confidence: 99%

Influences of Dietary Deoxycholic Acid on Progression of Hepatocellular Neoplasms and Expression of Glutathione S-Transferases in Rats

et al. 1994

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Serial magnetic resonance imaging (MRI) was used to evaluate the influences of dietary deoxycholic acid (DCA) on the rate of progression of chemically induced hepatocellular neoplasms in rats. Male Fischer-344 rats with established persistent hepatocellular nodules generated by the Solt-Farber protocol were exposed to dietary DCA (0.3%) between 6 and 12 mo of age. Growth of nodules and carcinomas in vivo was measured by morphometric quantification of tumor images obtained every 6 wk. The final stages of neoplastic progression were determined by terminal histopathological examination and by expression and functional evaluation of glutathione S-transferase (GST) isoenzyme phenotypes. Dietary DCA increased the number of hepatocellular neoplasms per rat, accelerated the rate of growth of persistent nodules, and increased the histological progression of liver tumors. Expression of immunoreactive GST subunits Yf, Ya, and Yb 1 was induced in early persistent nodules, a pattern that was maintained throughout the study in both basal diet and DCA-fed groups. However, 5% of early nodules and about 75% of advanced neoplasms were partially or completely deficient in GST Yb2 expression in both groups. DCA did not alter the cytosolic activity for the GST substrates 1-chloro-2,4-dinitrobenzene (CDNB) or trans -4-phenyl-3-buten-2-one (tPBO) in tumors or surrounding liver. However, in both groups, CDNB activity was increased in the tumors relative to the surrounding nonneoplastic tissue, whereas activity for tPBO, a substrate more specific for the Yb2 subunit, was reduced in the tumors. All advanced neoplasms were similarly more resistant than surrounding liver to DNA-binding metabolites of aflatoxin B 1 or benzo [a]pyrene. These data demonstrate that DCA can increase the progression of established hepatocellular nodules to larger, more advanced neoplasms but does not preferentially select for a specific GST phenotype. Preferential loss of constitutively expressed GST Yb2 in both basal diet and DCA-fed groups may be an important aspect of progression from resistant nodules to advanced cancers in this model. These studies also demonstrate that serial MRI is a useful tool for measuring the rates of enlargement and patterns of growth in established hepatocellular neoplasms.

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“…The other kidney was used as control; Right: Delayed contrast enhanced MR image acquired 10 minutes after administration of the contrast medium. On both sequences the ablated lesion appeared as hypoenhanced zone (arrows) [14] The detection of tumors can be enhanced by applying intracellular contrast media such as mangafodipir trisodium (Mn-DPDP) [64] and bis-Gd-DTPA-mesoporphyrin (Gadophrin-2) [65]. In 14 liver ablation patients, Joarder et al assessed the number of lesions, ease of puncture planning, conspicuity of lesions, gallbladder, vessels, and surrounding bowel after administration of Mn-DPDP.…”

Section: Contrast Media Distributionmentioning

confidence: 99%

Potentials, Limitations and Future Directions of MR Contrast Media in Ablation Therapies

Saeed¹,

Liang²,

Hetts³

et al. 2016

Journal of Advances in Radiology and Medical Imaging

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Magnetic Resonance Imaging, Microangiography, and Histology in a Rat Model of Primary Liver Cancer

Cited by 38 publications

References 0 publications

Comparison between areas with Gd‐EOB‐DTPA uptake and without in hepatocellular carcinomas on Gd‐EOB‐DTPA‐enhanced hepatobiliary‐phase MR imaging: Pathological correlation

Comparison between areas with Gd‐EOB‐DTPA uptake and without in hepatocellular carcinomas on Gd‐EOB‐DTPA‐enhanced hepatobiliary‐phase MR imaging: Pathological correlation

Influences of Dietary Deoxycholic Acid on Progression of Hepatocellular Neoplasms and Expression of Glutathione S-Transferases in Rats

Potentials, Limitations and Future Directions of MR Contrast Media in Ablation Therapies

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