Magnetic Resonance Imaging Visualization of Hyaluronidase in Ovarian Carcinoma

Shiftan, Liora; Israely, Tomer; Cohen, M. D.; Frydman, Veronica; Dafni, Hagit; Stern, Robert; Neeman, Michal

doi:10.1158/0008-5472.can-04-3947

Cited by 57 publications

(63 citation statements)

References 40 publications

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“…Unfortunately, the normal spread of relaxation times was too high and unspecific to allow cancer identification using these variables. A large number of Gd-labeled vector molecules have also been suggested as markers for cancer by 1 H MRI (28). All these molecules are designed to fit into receptors on the cell surface.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Imaging by Hyperpolarized 13C Magnetic Resonance Imaging for In vivo Tumor Diagnosis

Golman¹,

Zandt²,

Lerche³

et al. 2006

Cancer Research

560

632

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The ''Warburg effect,'' an elevation in aerobic glycolysis, may be a fundamental property of cancer cells. For cancer diagnosis and treatment, it would be valuable if elevated glycolytic metabolism could be quantified in an image in animals and humans. The pyruvate molecule is at the metabolic crossroad for energy delivery inside the cell, and with a noninvasive measurement of the relative transformation of pyruvate into lactate and alanine within a biologically relevant time frame (seconds), it may be possible to quantify the glycolytic status of the cells. We have examined the metabolism after i.v. injection of hyperpolarized 13 C-pyruvate in rats with implanted P22 tumors. The strongly enhanced nuclear magnetic resonance signal generated by the hyperpolarization techniques allows mapping of pyruvate, lactate, and alanine in a 5 Â 5 Â 10 mm 3 imaging voxel using a 1.5 T magnetic resonance scanner. The magnetic resonance scanning (chemical shift imaging) was initiated 24 seconds after the pyruvate injection and had a duration of 14 seconds. All implanted tumors showed significantly higher lactate content than the normal tissue. The results indicate that noninvasive quantification of localized Warburg effect may be possible.

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Section: Discussionmentioning

confidence: 99%

Metabolic Imaging by Hyperpolarized 13C Magnetic Resonance Imaging for In vivo Tumor Diagnosis

Golman¹,

Zandt²,

Lerche³

et al. 2006

Cancer Research

560

632

View full text Add to dashboard Cite

show abstract

“…146 Hyaluronidase activity was detected by MRI in an ovarian tumor model to assess peritumor angiogenic balance. 152 Until today, targeted contrast agents used for molecular imaging by MRI have only been used in preclinical applications, however, the proof of principle studies in preclinical settings have opened avenues for novel targets for MRI in the clinical assessment of cancer patients.…”

Section: Molecular Imaging With Mri In Cancer Imagingmentioning

confidence: 99%

Using magnetic resonance imaging and spectroscopy in cancer diagnostics and monitoring

Kauppinen

Peet

2011

Cancer Biology & Therapy

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“…67 Mazooz et al 68 described a Gd-DTPA peptide acting as a transglutaminase substrate, which was used to monitor transglutaminase activity. Shiftan et al reported MR imaging visualization of hyaluronidase in ovarian carcinoma, 69 related to the aggressiveness of ovarian cancer metastasis. Chen et al 70 visualized plaque rupture in atherosclerosis with a Gd-DOTAϪ serotonin derivative, which polymerizes in the presence of neutrophil myeloperoxidase, resulting in a remarkable relaxivity increase.…”

Section: Ph-sensitive Probesmentioning

confidence: 99%

Smart MR Imaging Agents Relevant to Potential Neurologic Applications

Bonnet¹,

Tóth²

2009

AJNR Am J Neuroradiol

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SUMMARY: Molecular imaging is aimed at the noninvasive visualization of the expression and function of bioactive molecules that often represent specific molecular signatures in disease processes. Any molecular imaging procedure requires an imaging probe that is specific to a given molecular event, which puts an important emphasis on chemistry development. In MR imaging, the past years have witnessed significant advances in the design of molecular agents, though most of these efforts have not yet progressed to in vivo studies. In this review, we present some examples relevant to potential neurobiologic applications. Our aim was to show what chemistry can bring to the area of molecular MR imaging with a focus on the 2 main classes of imaging probes: Gd 3ϩ -based and PARACEST agents. We will discuss responsive probes for the detection of metal ions such as Ca, Zn, Fe, and Cu, pH, enzymatic activity, and oxygenation state.ABBREVIATIONS: BBB ϭ blood-brain barrier; BOLD ϭ blood oxygen levelϪdependent; bpy ϭ bipyridine; C, carbon; Ca ϭ calcium; CEST ϭ chemical exchange saturation transfer; CO 3 ϭ carbonate; Cu ϭ copper; DTPA ϭ diethylene-triamine pentaacetic acid; DOTA ϭ 1,4,7,10-tetraaza-1,4,7,10-tetrakis(carboxymethyl)cyclododecane; Eu ϭ europium; Fe ϭ iron; Gd ϭ gadolinium; H ϭ hydrogen; HSA ϭ human serum albumin; k ex ϭ exchange rate; M ϭ metal ion; Mn ϭ manganese; N ϭ nitrogen; O ϭ oxygen; OH ϭ hydroxide; PARACEST ϭ paramagnetic CEST; PO 4 ϭ phosphate; q ϭ number of water molecules coordinated to Gd 3ϩ ; T 1,2e ϭ electron spin relaxation times; TPEN ϭ N,N,NЈ,NЈ-tetrakis(2-pyridylmethyl)ethylene-diamine; tpps ϭ 5,10,15,20-tetrakis-(p-sulfonatophenyl porphinate; tpy ϭ terpyridine; Yb ϭ ytterbium; Zn ϭ zinc; tau R ϭ reorientational correlation time of the molecule M olecular imaging is an emerging science area aimed at noninvasive visualization of the expression and function of bioactive molecules that often represent specific molecular signatures in disease processes. It seeks the biochemical or physiologic abnormalities underlying the disease, rather than the structural consequences of these abnormalities. Potential clinical applications of molecular imaging have been widely recognized, though the limited sensitivity and specificity of current molecular imaging approaches are often a major roadblock for clinical applications. Besides clinical settings, drug development can also largely benefit from the integration of molecular imaging. Molecular imaging not only allows rationalization of the parameters related to disease processes but it can also replace the common invasive research techniques (such as histology, which requires sacrificing animals for each time point in the experiment) by time-effective repeatable real-time visualization of biologically relevant processes. Currently, progress in the molecular imaging area is simultaneously moving at 3 levels: 1) improving the imaging hardware for use in preclinical and clinical settings, 2) identifying and validating new biologically relevant imaging targets, and 3...

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Magnetic Resonance Imaging Visualization of Hyaluronidase in Ovarian Carcinoma

Cited by 57 publications

References 40 publications

Metabolic Imaging by Hyperpolarized 13C Magnetic Resonance Imaging for In vivo Tumor Diagnosis

Metabolic Imaging by Hyperpolarized 13C Magnetic Resonance Imaging for In vivo Tumor Diagnosis

Using magnetic resonance imaging and spectroscopy in cancer diagnostics and monitoring

Smart MR Imaging Agents Relevant to Potential Neurologic Applications

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