Magnetic silica/titania xerogel applied as electrochemical biosensor for catechol and catecholamines

Morawski, Franciele de Matos; Deon, Marion; Nicolodi, Sabrina; Menezes, Eliana Weber de; Costa, Tânia Maria Haas; Dias, Susana Oliveira; Benvenutti, Edílson V.; Arenas, Leliz Ticona

doi:10.1016/j.electacta.2018.01.127

Cited by 33 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melatonin (N-acetyl-5-methoxytryptamine) as an effective antioxidant [ 61 ] could be ultimately oxidized to 5-methoxyindole-3-acetic acid [ 62 ]. Hence, the level of 5-methoxyindole-3-acetic acid in Group H with higher RDP density was significantly higher than that in Group C. In this study, catechol as an antioxidant [ 63 , 64 ] was positively related to the relative abundances of Ruminococcus _2 and Prevotella_ 1, which might imply that high energy and protein levels in Group H could enhance the catechol concentration by these two genera. Xue et al [ 65 ] reported that the content of spermidine increased in the group of high concentrate diets (rich in starch), which is in line with the results of our study.…”

Section: Discussionmentioning

confidence: 57%

Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density

Wang

Peng

Xiao

et al. 2020

Animals

View full text Add to dashboard Cite

This study was conducted to investigate the impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites. A total of 12 ruminal samples were collected from Shaanbei white cashmere goats which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) and control (Group C; CP: 8.73%; ME: 8.60 MJ/kg) groups. Thereby, 16S rRNA gene sequencing and a quantitative polymerase chain reaction were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera Prevotella_1 and Succiniclasticum, while decreasing the number of Proteobacteria (p < 0.05). The dominant differential metabolites were amino acids, peptides, and analogs. Tyrosine metabolism played an important role among the nine main metabolic pathways. Correlation analysis revealed that both Prevotella_1 (r = 0.608, p < 0.05) and Ruminococcus_2 (r = 0.613, p < 0.05) showed a positive correlation with catechol. Our findings revealed that the diets with high energy and protein levels in Group H significantly altered the composition of ruminal bacteria and metabolites, which can help to improve the dietary energy and protein use efficiency in goats.

show abstract

Section: Discussionmentioning

confidence: 57%

Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density

Wang

Peng

Xiao

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…Melatonin (Nacetyl-5-methoxytryptamine) as an effective antioxidant [70] could be ultimately oxidized to 5methoxyindole-3-acetic acid [71]. Hence, the level of 5-methoxyindole-3-acetic acid in Group H with higher protein diets was signi cantly higher than that in Group C. In this study, catechol as an antioxidant [72,73] was positively related to the relative abundances of Ruminococcus_2 and Prevotella_1, which might imply that high energy and protein levels in Group H could enhance the catechol concentration by these two genera. Xue et al [74] reported that the content of spermidine increased in the group of high concentrate diets compared with moderate concentration of diets, which is in line with our study.…”

Section: Comparison Of the Composition And Differences Of Ruminal Metmentioning

confidence: 63%

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Wang

Tang

Xiao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Dietary energy and protein play important roles in rumen fermentation. However, the comprehensive impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites were largely unknown. Therefore, the objective of the current study was to investigate the changes in rumen bacterial community and metabolites in response to the diets with simultaneous changes of dietary energy and protein levels in Shaanbei white cashmere goats (SWCG). Methods: A total of 12 ruminal samples were collected from SWCG, which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) or control (Group C; CP: 8.73%; ME:8.60 MJ/kg) groups. The experiment lasted for 65 days, including 10 days for adaptation. 16S rRNA gene sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. Results: The study observed 539 genera belonging to 30 phyla, which were distributed throughout the rumen samples. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera Prevotella_1 and Succiniclasticum, while decreased the number of phylum Proteobacteria (p<0.05). Among the 24 differential metabolites (VIP>1.0, p<0.05) detected in this study, the dominant differential metabolites were amino acids, peptides and analogs. Tyrosine metabolism played an important role among the 9 main metabolic pathways. Correlation analysis revealed that Prevotella_1 showed strong positive correlation with 5-methoxyindole-3-acetic acid (r=0.601, p<0.05) and catechol (r=0.608, p<0.05). Succiniclasticum was positively correlated with 2-ketoadipate (r=0.741, p<0.01). Conclusions: Our findings revealed that the diets with high energy and protein in Group H significantly altered the composition of ruminal bacteria and metabolites, which could help to improve the dietary energy and protein use efficiency in goats.

show abstract

“…Several bio-recognition components have been used to detect polyphenols, such as microorganisms [ 23 ], DNA [ 24 ], whole cells [ 18 ], anti-bodies, and enzymes [ 25 ]. For monitoring PhCs, enzymatic biosensors based on laccase (Lac) [ 26 ], horseradish peroxidase [ 27 ], and tyrosinase [ 28 , 29 ] have proven to be the most effective due to their ability to directly catalyze electron transfer reactions without introducing cofactors into the reaction medium. Different supporting matrices such as conducting polymers, metal and metal oxide nanoparticles, carbon nano tubes (CNTs), graphite, silica gel, activated charcoal, reduced graphene oxide (RGO), glass surfaces, etc., have been used for the immobilization of these enzymes [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Prospects of Carbonaceous Nanomaterials-Based Laccase Biosensor for Electrochemical Detection of Phenolic Compounds

et al. 2023

View full text Add to dashboard Cite

Phenolic compounds (PhCs) are ubiquitously distributed phytochemicals found in many plants, body fluids, food items, medicines, pesticides, dyes, etc. Many PhCs are priority pollutants that are highly toxic, teratogenic, and carcinogenic. Some of these are present in body fluids and affect metabolism, while others possess numerous bioactive properties such as retaining antioxidant and antimicrobial activity in plants and food products. Therefore, there is an urgency for developing an effective, rapid, sensitive, and reliable tool for the analysis of these PhCs to address their environmental and health concern. In this context, carbonaceous nanomaterials have emerged as a promising material for the fabrication of electrochemical biosensors as they provide remarkable characteristics such as lightweight, high surface: volume, excellent conductivity, extraordinary tensile strength, and biocompatibility. This review outlines the current status of the applications of carbonaceous nanomaterials (CNTs, graphene, etc.) based enzymatic electrochemical biosensors for the detection of PhCs. Efforts have also been made to discuss the mechanism of action of the laccase enzyme for the detection of PhCs. The limitations, advanced emerging carbon-based material, current state of artificial intelligence in PhCs detection, and future scopes have also been summarized.

show abstract

Magnetic silica/titania xerogel applied as electrochemical biosensor for catechol and catecholamines

Cited by 33 publications

References 58 publications

Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density

Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Recent Prospects of Carbonaceous Nanomaterials-Based Laccase Biosensor for Electrochemical Detection of Phenolic Compounds

Contact Info

Product

Resources

About