2004
DOI: 10.1186/1477-044x-2-7
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Magnetic techniques for the isolation and purification of proteins and peptides

Abstract: Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of m… Show more

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Cited by 447 publications
(198 citation statements)
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“…[1][2][3][4][5][6] Recently, graphene-nanocomposite magnetic nanoparticles have been developed as an alternative to the application of carbon-based nanomaterials in the environment. [7][8][9][10][11][12] Typically, magnetic nanoparticles are dispersed into a sample solution to adsorb target compounds through the interaction of graphene with analytes.…”
Section: Introductionmentioning
confidence: 99%
“…[1][2][3][4][5][6] Recently, graphene-nanocomposite magnetic nanoparticles have been developed as an alternative to the application of carbon-based nanomaterials in the environment. [7][8][9][10][11][12] Typically, magnetic nanoparticles are dispersed into a sample solution to adsorb target compounds through the interaction of graphene with analytes.…”
Section: Introductionmentioning
confidence: 99%
“…The use of inhomogeneous magnetic fields to take away the magnetic particles from a solution is known as magnetic separation or magnetophoresis and has provided new techniques capable of improving standard technologies, especially in biotechnological applications. 34 As a result, the manipulation of magnetic particles by using inhomogeneous magnetic fields is a topic of great interest in a wide range of research and technological areas. 29,35 The clustering dynamics of magnetic particles in steric 36 and ionic 9,37 ferrofluids was previously investigated by means of Raman and NMR spectroscopy.…”
Section: Introductionmentioning
confidence: 99%
“…Magnetic separation in an MSFB can facilitate the separation and purification processes. 19,20 26 }, and ion-exchange chromatography (Safarik et al 27 used magnetic macroporous cellulose cation exchangers for lysozyme separation). However, the severe limitations of many of these methods, including long processing steps, high cost, and dilution of egg white during processing, have hampered their applications.…”
Section: Introductionmentioning
confidence: 99%