Magnetically Directed Self-Assembly of Electrospun Superparamagnetic Fibrous Bundles to Form Three-Dimensional Tissues with a Highly Ordered Architecture

Lee, Wen Yu; Cheng, Wei Yuan; Yeh, Yi Chun; Lai, Chih‐Huang; Hwang, Shiaw Min; Hsiao, Chun Wen; Huang, Chia Wen; Chen, Mei-Chin; Sung, Hsing‐Wen

doi:10.1089/ten.tec.2010.0621

Cited by 27 publications

(15 citation statements)

References 41 publications

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“…C2C12 murine myoblast has been widely applied to in vitro studies on skeletal muscle damage, regeneration, and differentiation, as well as chemical, electrical and mechanical stimulation due to the cell line's rapid growth, ease of culture, and sensitivity to manipulations [ 10 – 18 ]. In addition, it has been extensively used for the production of 3D tissue-engineered muscle [ 19 – 24 ]. Previously, it was declared that the C2C12 cell line exhibited OC behavior [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism

Chen

et al. 2013

BioMed Research International

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Background. Oxygen and glucose are two important nutrients for mammalian cell function. In this study, the effect of glucose and oxygen concentrations on C2C12 cellular metabolism was characterized with an emphasis on detecting whether cells show oxygen conformance (OC) in response to hypoxia. Methods. After C2C12 cells being cultured in the levels of glucose at 0.6 mM (LG), 5.6 mM (MG), or 23.3 mM(HG) under normoxic or hypoxic (1% oxygen) condition, cellular oxygen consumption, glucose consumption, lactate production, and metabolic status were determined. Short-term oxygen consumption was measured with a novel oxygen biosensor technique. Longer-term measurements were performed with standard glucose, lactate, and cell metabolism assays. Results. It was found that oxygen depletion in normoxia is dependent on the glucose concentration in the medium. Cellular glucose uptake and lactate production increased significantly in hypoxia than those in normoxia. In hypoxia the cellular response to the level of glucose was different to that in normoxia. The metabolic activities decreased while glucose concentration increased in normoxia, while in hypoxia, metabolic activity was reduced in LG and MG, but unchanged in HG condition. The OC phenomenon was not observed in the present study. Conclusions. Our findings suggested that a combination of low oxygen and low glucose damages the viability of C2C12 cells more seriously than low oxygen alone. In addition, when there is sufficient glucose, C2C12 cells will respond to hypoxia by upregulating anaerobic respiration, as shown by lactate production.

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Section: Introductionmentioning

confidence: 99%

Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism

Chen

et al. 2013

BioMed Research International

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“…[10][11][12] In particular, current advanced techniques in drug targeting use delicate surface modifications for the conjugation of antiangiogenic and anticancer drugs. 13,14 SPIONs have the advantage of easy transportation in vivo to the desired site by an external electrical magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups

Hong¹,

Lee²,

Lee³

et al. 2011

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely utilized for the diagnosis and therapy of specific diseases, as magnetic resonance imaging (MRI) contrast agents and drug-delivery carriers, due to their easy transportation to targeted areas by an external magnetic field. For such biomedical applications, SPIONs must have multifunctional characteristics, including optimized size and modified surface. However, the biofunctionality and biocompatibility of SPIONs with various surface functional groups of different sizes have yet to be elucidated clearly. Therefore, it is important to carefully monitor the cytotoxicity and genotoxicity of SPIONs that are surfaced-modified with various functional groups of different sizes. In this study, we evaluated SPIONs with diameters of approximately 10 nm and 100~150 nm, containing different surface functional groups. SPIONs were covered with −O − groups, so-called bare SPIONs. Following this, they were modified with three different functional groups – hydroxyl (−OH), carboxylic (−COOH), and amine (−NH 2 ) groups – by coating their surfaces with tetraethyl orthosilicate (TEOS), (3-aminopropyl)trimethoxysilane (APTMS), TEOS-APTMS, or citrate, which imparted different surface charges and sizes to the particles. The effects of SPIONs coated with these functional groups on mitochondrial activity, intracellular accumulation of reactive oxygen species, membrane integrity, and DNA stability in L-929 fibroblasts were determined by water-soluble tetrazolium, 2′,7′-dichlorodihydrofluorescein, lactate dehydrogenase, and comet assays, respectively. Our toxicological observations suggest that the functional groups and sizes of SPIONs are critical determinants of cellular responses, degrees of cytotoxicity and genotoxicity, and potential mechanisms of toxicity. Nanoparticles with various surface modifications and of different sizes induced slight, but possibly meaningful, changes in cell cytotoxicity and genotoxicity, which would be significantly valuable in further studies of bioconjugation and cell interaction for drug delivery, cell culture, and cancer-targeting applications.

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“…One of the challenges in engineering 3D cell-dense tissues is the conflicting objectives of increasing porosity within fibrous scaffolds to improve cellular infiltration and the negative effect that this has on fiber alignment [120]. To address these issues an electrospinning technique was used to fabricate fibrous bundles consisting of composite fibers of poly( l -lactic- co -glycolic) acid and MNPs.…”

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Magnetic Nanoparticle-Based Approaches to Locally Target Therapy and Enhance Tissue Regeneration In Vivo

et al. 2012

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Magnetic-based systems utilizing superparamagnetic nanoparticles and a magnetic field gradient to exert a force on these particles have been used in a wide range of biomedical applications. This review is focused on drug targeting applications that require penetration of a cellular barrier as well as strategies to improve the efficacy of targeting in these biomedical applications. Another focus of this review is regenerative applications utilizing tissue engineered scaffolds prepared with the aid of magnetic particles, the use of remote actuation for release of bioactive molecules and magneto–mechanical cell stimulation, cell seeding and cell patterning.

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Magnetically Directed Self-Assembly of Electrospun Superparamagnetic Fibrous Bundles to Form Three-Dimensional Tissues with a Highly Ordered Architecture

Cited by 27 publications

References 41 publications

Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism

Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism

Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups

Magnetic Nanoparticle-Based Approaches to Locally Target Therapy and Enhance Tissue Regeneration In Vivo

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