Magnetically Guided Viral Transduction of Gene-Based Sensitization for Localized Photodynamic Therapy To Overcome Multidrug Resistance in Breast Cancer Cells

Liao, Zi-Xian; Kempson, Ivan M.; Fa, Yu-Chen; Liu, Meng-Chia; Hsieh, Li-Chen; Huang, Kuo‐Yen; Wang, Lifeng

doi:10.1021/acs.bioconjchem.7b00162

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…Furthermore, functionalized AAV2 nanoparticles can decrease the using dose, the risks of AAV-directed immune response, ectopic expression, and oncogene activation. Magnetic nanoparticles (MNPs) can accelerate vector accumulation at target sites and enhance virion infectivity via magnetic-field-enforced delivery [ 93 ]. As a result, a recombinant AAV2 carrying the KillerRed gene (AAV2-KR) chemically conjugated with iron oxide nanoparticles is designed to be guided in a magnetic field.…”

Section: Comprehensive Therapymentioning

confidence: 99%

Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications

Liu

Wang

Yang

et al. 2021

IJMS

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Photodynamic therapy (PDT) is a clinical treatment for cancer or non-neoplastic diseases, and the photosensitizers (PSs) are crucial for PDT efficiency. The commonly used chemical PSs, generally produce ROS through the type II reaction that highly relies on the local oxygen concentration. However, the hypoxic tumor microenvironment and unavoidable dark toxicity of PSs greatly restrain the wide application of PDT. The genetically encoded PSs, unlike chemical PSs, can be modified using genetic engineering techniques and targeted to unique cellular compartments, even within a single cell. KillerRed, as a dimeric red fluorescent protein, can be activated by visible light or upconversion luminescence to execute the Type I reaction of PDT, which does not need too much oxygen and surely attract the researchers’ focus. In particular, nanotechnology provides new opportunities for various modifications of KillerRed and versatile delivery strategies. This review more comprehensively outlines the applications of KillerRed, highlighting the fascinating features of KillerRed genes and proteins in the photodynamic systems. Furthermore, the advantages and defects of KillerRed are also discussed, either alone or in combination with other therapies. These overviews may facilitate understanding KillerRed progress in PDT and suggest some emerging potentials to circumvent challenges to improve the efficiency and accuracy of PDT.

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Section: Comprehensive Therapymentioning

confidence: 99%

Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications

Liu

Wang

Yang

et al. 2021

IJMS

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“…gated to iron oxide NPs were remotely guided and delivered to the tumor site with microscale precision, where phototoxicity was then triggered by irradiation. 489,502,503 Recently, Tseng et al further developed this approach with an elaborate hybrid design. AAV encoding the photosensitive apoptosis-inducing protein was conjugated to SPIONs and loaded to a nitroimidazole conjugated hyaluronic acid NP containing lactate oxidase.…”

Section: Biomaterials Science Reviewmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…For instance, a nanoscale coordinated copolymer (NCPs) was designed to entrap Ce6 and DOX in a single nanoplatform. Upon light exposure, the resultant NCPs-DOX-Ce6 PEG NPs triggered simultaneous release of entrapped drug and PDT via generation of singlet oxygen for synergistic chemo-photodynamic therapy 26 , 27 . Several other similar examples of synergistic combination therapy using various chemotherapeutic anticancer drugs and phototherapeutic modules with improved combined therapeutic effect have been reported recently 28 , 29 .…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of porphyrin-grafted lipid into nanoparticles encapsulating doxorubicin for synergistic chemo-photodynamic therapy and fluorescence imaging

et al. 2018

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The limited clinical efficacy of monotherapies in the clinic has urged the development of novel combination platforms. Taking advantage of light-triggered photodynamic treatment combined together with the controlled release of nanomedicine, it has been possible to treat cancer without eliciting any adverse effects. However, the challenges imposed by limited drug loading capacity and complex synthesis process of organic nanoparticles (NPs) have seriously impeded advances in chemo-photodynamic combination therapy. In this experiment, we utilize our previously synthesized porphyrin-grafted lipid (PGL) NPs to load highly effective chemotherapeutic drug, doxorubicin (DOX) for synergistic chemo-photodynamic therapy.Methods: A relatively simple and inexpensive rapid injection method was used to prepare porphyrin-grafted lipid (PGL) NPs. The self-assembled PGL NPs were used further to encapsulate DOX via a pH-gradient loading protocol. The self-assembled liposome-like PGL NPs having a hydrophilic core were optimized to load DOX at an encapsulation efficiency (EE) of ~99%. The resultant PGL-DOX NPs were intact, highly stable and importantly these NPs successfully escaped from the endo-lysosomal compartment after laser irradiation to release DOX in the cytosol. The therapeutic efficacy of the aforementioned formulation was validated both in vitro and in vivo.Results: PGL-DOX NPs demonstrated excellent cellular uptake, chemo-photodynamic response, and fluorescence imaging ability in different cell lines. Under laser irradiation, cells treated with a low molar concentration of PGL-DOX NPs reduced cell viability significantly. Moreover, in vivo experiments conducted in a xenograft mouse model further demonstrated the excellent tumor accumulation capability of PGL-DOX NPs driven by the enhanced permeability and retention (EPR) effect. Through fluorescence imaging, the biodistribution of PGL-DOX NPs in tumor and major organs was also easily monitored in real time in vivo. The inherent ability of porphyrin to generate ROS under laser irradiation combined with the cytotoxic effect of the anticancer drug DOX significantly suppressed tumor growth in vivo.Conclusion: In summary, the PGL-DOX NPs combined chemo-photodynamic nanoplatform may serve as a potential candidate for cancer theranostics.

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Magnetically Guided Viral Transduction of Gene-Based Sensitization for Localized Photodynamic Therapy To Overcome Multidrug Resistance in Breast Cancer Cells

Cited by 12 publications

References 18 publications

Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications

Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications

Materials promoting viral gene delivery

Self-assembly of porphyrin-grafted lipid into nanoparticles encapsulating doxorubicin for synergistic chemo-photodynamic therapy and fluorescence imaging

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