Magnetically Responsive Horseradish peroxidase@ZIF-8 for Biocatalysts

Abstract

<div>Here, we studied a catalytically active and magnetically responsive porous bio-composite obtained from the synthesis of ZIF-8 in presence of iron oxide magnetic nanoparticles and horseradish peroxidase (HRP) enzyme as guest species. Using a one-pot approach in water the precursors of ZIF-8 (zinc acetate and 2-methylimidazole) spontaneously self-assembles around the guest species. We characterized the composite by means of XRD, SEM, FTIR, AFM, and CLSM. SAXS investigation of the kinetics of crystallization showed how the presence of the guest species can act as nucleation seeds. Moreover, we found that the bio-catalytic activity of the HRP/MNP@ZIF-8 biocomposite is 5 times higher than the analoguous composite without MNPs.</div>

Magnetically Responsive Enzyme and Hydrogen-bonded Organic Framework Biocomposites for Biosensing

“…Amide I, 1700-1600 cm-1) [34] and Fe3O4 (e.g. Fe-O stretching, 600-500 cm-1) [15]. This set of vibrational modes confirms the formation of a BioHOF-1 with immobilized enzymes and MNPs (MNPs-CAT@BioHOF-1).…”

“…[10][11][12][13][14] We note that, on a laboratory scale, the combination of enzymes and magnetic responsiveness has been successfully applied to the synthesis of fine chemicals, biosensing, and lab-on-a-chip devices. [10,[15][16][17][18] As a result, enzyme immobilization in magnetically active porous materials is a fervent research field. [2,19,20] investigation and design of enzyme@HOF systems for biocatalysis and biosensing applications.…”

“…we can collect them from a solution by using a permanent magnet) and they were previously successfully integrated in enzymebased composite materials. [15,28] Catalase (CAT), an iron-heme enzyme that catalyses the decomposition of hydrogen peroxide to water and oxygen, was selected as model enzyme. [21,25,29] In addition, glucose oxidase (GOx) was explored as it is an oxidoreductase enzyme that is widely used for biotechnological application including biosensors for biomedical and food processing applications.…”