This paper discusses an investigation of the influence of dispersion impact on mixed convection flow over a horizontal cone within a non-Darcy porous medium subjected to convective boundary conditions. By imposing appropriate similarity transformations, the nonlinear partial differential equations governing flow, temperature, concentration, and microbe fields are reduced to a system of ordinary differential equations, which are then solved using the MATLAB BVP4C function. In a few circumstances, the research is brought to a strong conclusion by comparing the findings of the current study to previously published works. Mixed convection parameter λ, buoyancy parameters N1,N2, Lewis parameter Le, bioconvection lewis parameter Lb, Bioconvection peclet number Pe, Biot number Bi, Biot number of Mass transfer Bi,m and also Biot number of motile microorganism transfer Bi,n are all numerically calculated for various values of the dimensionless parameters of the problem. The results also reveal that, in the presence of dispersion effects, these parameters greatly influence the heat, mass, and motile microorganism transfer rates, as well as the corresponding velocity, temperature, concentration, and motile microorganism profiles.