Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo

Scherer, Franz; Anton, Martina; Schillinger, Ulrike; Henke, Julia; Bergemann, Christian; Krüger, Achim; Gänsbacher, Bernd; Plank, Christian

doi:10.1038/sj.gt.3301624

Cited by 871 publications

(651 citation statements)

References 24 publications

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“…The physical forces employed to increase the encounter frequency between cells and retroviruses are gravitational, 6,7 convective, 8 and magnetic. 9 It should be noted that these approaches are basically designed for anchorage-dependent cells rather than suspension cells. As a Polybrene (').…”

Section: Discussionmentioning

confidence: 99%

“…5 The probability that retroviruses encounter the target cells before they decay is, therefore, strongly dependent on their initial position relative to the target cells. Attempting to overcome the limitations of a short retroviral half-life and an infection route dependent on the Brownian motion of retrovirus, methods of forcing viral particles to the layer of anchorage-dependent cells by centrifugation, 6,7 convective flow of virus-containing medium, 8 and magnetofection 9 have all been demonstrated to improve the efficiency of gene transduction. However, the aforementioned strategies cannot be easily adopted for large-scale settings.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced retroviral gene delivery in ultrasonic standing wave fields

Lee

Peng

2005

Gene Ther

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Enhancement of retroviral transduction efficiency has been achieved by several physical and chemical approaches. However, the application of those methods is hampered by not easily scalable configurations. In this study, instead of looking into the effect of sonoporation, the potential of ultrasonic standing wave fields (USWF) to facilitate retroviral transduction rate was explored. We reasoned that, driven by the primary acoustic radiation force, suspended cells moved to the pressure nodal planes first and formed cell bands. Nanometer-sized retroviruses, circulated between nodal planes by acoustic microstreaming, then used the preformed cell bands as the nucleating sites to attach on. As a result, the encounter opportunity between retroviruses and cells was increased and further facilitated the gene delivery efficiency. Our results showed that mega-Hertz USWF brought K562 erythroleukemia cells (10 6 cells/ml) and vesicular stomatitis virus G-protein (VSV-G) pseudotyped retroviruses (titer of 5 Â 10 6 CFU/ml) into close contact at the pressure nodal planes, yielding a four-fold increment of enhanced green fluorescent protein transgene expression after 5-min USWF exposure in the presence of Polybrene. Furthermore, with a fixed titer of retrovirus, the transduction rate was augmented with the increase of cell concentration. In summary, USWF offer a feasible means to enhance retroviral transduction efficiency in large-scale settings. Gene Therapy (2005) 12, 625-633.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced retroviral gene delivery in ultrasonic standing wave fields

Lee

Peng

2005

Gene Ther

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“…Application of magnetic fields following local injection of plasmidassociated nanoparticles (magnetofection) has been reported to enhance in vivo gene transfer to the gastrointestinal tract and the vasculature of the ear. 66 The improved gene transfer associated with magnetofection does not appear to be as substantial as that seen with hydrodynamic delivery or electroporation, although direct comparisons have not yet been made.…”

Section: Clinically Applicable Ultrasound Can Enhance Plasmid-based Gmentioning

confidence: 99%

Gene Therapy Progress and Prospects: Electroporation and other physical methods

2004

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Over the last 5 years, physical methods of plasmid delivery have revolutionized the efficiency of nonviral gene transfer, in some cases reaching the efficiencies of viral vectors. In vivo electroporation dramatically increases transfection efficiency for a variety of tissues. Other methods with clinical precedent, pressure-perfusion and ultrasound, also improve plasmid gene transfer. Alternatives such as focused laser, magnetic fields and ballistic (gene gun) approaches can also enhance delivery. As plasmid DNA appears to be a safe gene vector system, it seems likely that plasmid with physically enhanced delivery will be used increasingly in clinical trials.

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“…These include projectile delivery (Yang et al, 1990), hydrodynamic delivery (Andrianaivo et al, 2004), electroporation (Mir et al, 1999), sonoporation (Bao et al, 1997), and magnetic forcemediated delivery Huth et al, 2004;Mah et al, 2002;Scherer et al, 2002). Magnetic force-mediated gene delivery, also termed "magnetofectionȍ, is related to the concept of magnetically targeted drug delivery (Meyers et al, 1963) and involves the application of a static magnetic field that guides magnetic particle (MP)-associated gene vectors to accumulate on the cell surface (Dobson, 2006;Mah et al, 2002;Scherer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Mah et al (2002) demonstrated efficient gene delivery both in vitro and in vivo in by using magnetic microsphere-bound adeno-associated virus (AAV). Scherer and coworkers have developed polyethylenimine (PEI)-coated MPs, and demonstrated efficient magnetofection in a variety of cell lines, as well as in excised airway epithelium (Huth et al, 2004;Scherer et al, 2002;Schillinger et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Pan

Guan

Yoo

et al. 2008

International Journal of Pharmaceutics

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Cationic lipid-coated magnetic nanoparticles (MPs) associated with transferrin were evaluated as gene transfer vectors in the presence of a static magnetic field. MPs were prepared by chemical precipitation and were surface-coated with cationic lipids, composed of DDAB/soy PC (60:40 mole/mole). These cationic MPs were then combined with polyethylenimine (PEI) condensed plasmid DNA, followed by transferrin. The resulting magnetic electrostatic complexes retained relatively compact particle size and showed complete DNA condensation. Their transfection activity in the presence of a static magnetic field was evaluated by luciferase and green fluorescent protein (GFP) reporter genes. The magnetic complexes exhibited up to 300-fold higher transfection activity compared to commonly used cationic liposomes or cationic polymer complexes, based on luciferase assay. The enhancement in transfection activity was maximized when the cells were exposed to the vectors for a relatively short period of time (15 min), or were treated in media containing 10% serum. Incorporation of transferrin further improved transfection efficiency of the cationic MPs. However, when cells were incubated for 4 h in serum-free media, magnetic and non-magnetic vectors showed similar transfection efficiencies. In conclusion, transferrin-associated cationic MPs are excellent gene transfer vectors that can mediate very rapid and efficient gene transfer in vitro in the presence of a magnetic field.

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Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo

Cited by 871 publications

References 24 publications

Enhanced retroviral gene delivery in ultrasonic standing wave fields

Enhanced retroviral gene delivery in ultrasonic standing wave fields

Gene Therapy Progress and Prospects: Electroporation and other physical methods

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

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