Magnetofection In Vivo by Nanomagnetic Carriers Systemically Administered into the Bloodstream

Sizikov, Artem A.; Никитин, П. И.; Nikitin, Maxim P.

doi:10.3390/pharmaceutics13111927

Cited by 20 publications

(7 citation statements)

References 115 publications

(173 reference statements)

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“…Another promising application of magnetic particles in gene therapy is the delivery of therapeutic constructs ex vivo. The use of magnetic nanoparticles can generally increase the efficiency of delivery into the cells [38].…”

Section: Discussionmentioning

confidence: 99%

Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

et al. 2022

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Suicidegene therapy is considered a promising approach for the treatment of uterine leiomyoma (UL), a benign tumor in women characterized by precise localization. In this study, we investigate the efficiency of αvβ3 integrin-targeted arginine-rich peptide carrier R6p-cRGD electrostatically bound to magnetic nanoparticles (MNPs) for targeted DNA delivery into the UL cells. The physico–chemical and cytotoxic properties, transfection efficiency, and specificity of R6p-cRGD/DNA/MNPs polyplexes were evaluated. The addition of MNPs resulted in a decrease in the time needed for successful transfection with simultaneous increase in efficiency. We revealed a therapeutic effect on primary UL cells after delivery of plasmid encoding the herpes simplex virus type 1 (HSV-1) thymidine kinase gene. Treatment with ganciclovir resulted in 20% efficiency of suicide gene therapy in UL cells transfected with the pPTK-1 plasmid. Based on these results, we conclude that the use of cationic peptide carriers with MNPs can be promising for the development of modular non-viral carriers for suicide gene delivery to UL cells.

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Section: Discussionmentioning

confidence: 99%

Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

et al. 2022

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“…MF is very efficient due to its swift targeting and decreased cytotoxicity, providing notable benefits compared to conventional chemical transfection techniques [157][158][159].…”

Section: Magnetofection (Mf)mentioning

confidence: 99%

Magnetic Nanoparticles: Synthesis, Characterization, and Their Use in Biomedical Field

Stiufiuc,

Stiufiuc

2024

Applied Sciences

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In recent years, the use of magnetic nanoparticles (MNPs) in biomedical applications has gained more and more attention. Their unusual properties make them ideal candidates for the advancement of diagnosis, therapy, and imaging applications. This review addresses the use of MNPs in the field of biomedicine encompassing their synthesis, biofunctionalization, and unique physicochemical properties that make them ideal candidates for such applications. The synthesis of magnetic nanoparticles involves a range of techniques that allow for control over particle size, shape, and surface modifications. The most commonly used synthesis techniques that play a crucial role in tailoring the magnetic properties of nanoparticles are summarized in this review. Nevertheless, the main characterization techniques that can be employed after a successful synthesis procedure are also included together with a short description of their biomedical applications. As the field of magnetic nanoparticles in biomedical applications is rapidly evolving, this review aims to serve as a valuable resource, especially for young researchers and medical professionals, offering basic but very useful insights into recent advancements and future prospects in this highly interdisciplinary research topic.

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“…Some approaches are realized by violating the integrity of cell membranes as a result of microinjection or electroporation [ 14 , 15 ], or using cell penetrating peptide [ 16 ]. Other methods are based on the use of various nanocarriers, such as inorganic materials, lipids, polymers, etc., [ 17 , 18 ]. These techniques are safer, but, with rare exceptions, may cause a decrease in cell viability [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Internalization of transferrin-tagged Myxococcus xanthus encapsulins into mesenchymal stem cells

Gabashvili,

Alexandrushkina,

Mochalova

et al. 2024

Exp. Biol. Med.

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Currently, various functionalized nanocarrier systems are extensively studied for targeted delivery of drugs, peptides, and nucleic acids. Joining the approaches of genetic and chemical engineering may produce novel carriers for precise targeting different cellular proteins, which is important for both therapy and diagnosis of various pathologies. Here we present the novel nanocontainers based on vectorized genetically encoded Myxococcus xanthus (Mx) encapsulin, confining a fluorescent photoactivatable mCherry (PAmCherry) protein. The shells of such encapsulins were modified using chemical conjugation of human transferrin (Tf) prelabeled with a fluorescein-6 (FAM) maleimide acting as a vector. We demonstrate that the vectorized encapsulin specifically binds to transferrin receptors (TfRs) on the membranes of mesenchymal stromal/stem cells (MSCs) followed by internalization into cells. Two spectrally separated fluorescent signals from Tf-FAM and PAmCherry are clearly distinguishable and co-localized. It is shown that Tf-tagged Mx encapsulins are internalized by MSCs much more efficiently than by fibroblasts. It has been also found that unlabeled Tf effectively competes with the conjugated Mx-Tf-FAM formulations. That indicates the conjugate internalization into cells by Tf-TfR endocytosis pathway. The developed nanoplatform can be used as an alternative to conventional nanocarriers for targeted delivery of, e.g., genetic material to MSCs.

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Magnetofection In Vivo by Nanomagnetic Carriers Systemically Administered into the Bloodstream

Cited by 20 publications

References 115 publications

Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

Magnetic Nanoparticles: Synthesis, Characterization, and Their Use in Biomedical Field

Internalization of transferrin-tagged Myxococcus xanthus encapsulins into mesenchymal stem cells

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