The resistivity, magnetoresistance (MR) and magnetization of double-layered manganite La 1.2 Sr 1.8-x Ca x Mn 2 O 7 (0.0 ≤ x ≤ 0.4) are studied to investigate the effect of substitution of Ca 2+ for Sr 2+ on the transport and magnetic properties. The resistivity data suggest that Ca doping results in the decrease of insulator-tometal transition temperature, T IM . The conduction mechanism above T IM is found to be of Mott variable range hopping type. The highest MR ratios (∆ρ/ρ 0 ) of 73% (H = 1.5 T) and 93% (H = 4 T) are observed for x = 0.3 sample at ~25 K. The variation of MR with temperature and applied magnetic field is discussed. A secondary transition at T * is observed in all the samples, which may be due to the twodimensional short-range ferromagnetic ordering.