“…Where fluorescent features, like microtubules, were separated well enough that their fluorescence signals convoluted with the LM point spread function did not overlap, we could identify individual tubes and correlate them with the cryo-EM image (Figs 3E and F, white arrowheads), providing images at <5 nm resolution of cellular regions chosen in the LM. Granted, there are now various LM techniques that beat the traditional resolution barrier imposed by the diffraction of light to visualize the location of selectively labelled features (Heintzmann & Ficz, 2006), such as nearfield microscopy (Baylis et al, 2007), structured illumination (Gustafsson, 2005) or LM superlenses (Liu et al, 2007;Smolyaninov et al, 2007), however, our correlative method uses commercially available tools and standard methods to provide information about cellular features in a near-native state at molecular resolution.…”