Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway

Alonso-Castro, Ángel Josabad; Zapata-Bustos, Rocio; Domínguez, Fabiola; García‐Carrancá, Alejandro; Salazar‐Olivo, Luis A.

doi:10.1016/j.phymed.2011.02.015

Cited by 58 publications

(41 citation statements)

References 19 publications

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“…We also showed that rexinoids honokiol and magnolol, isolated from Magnolia dealbata seeds, stimulated glucose uptake in insulin-sensitive and insulin-resistant murine and human adipocytes using the insulin signaling pathway (40). Our present results show that ISO is an additional natural compound with high capacity to induce the glucose uptake by insulin-targeted mammalian cells in spite of their responsiveness to this hormone.…”

Section: Discussionsupporting

confidence: 64%

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

et al. 2012

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Isoorientin (ISO) is a plant C-glycosylflavonoid with purported antidiabetic effects but unexplored mechanisms of action. To gain insight into its antidiabetic mechanisms, we assayed nontoxic ISO concentrations on the 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxy-D-glucose (2-NBDG) uptake by murine 3T3-F442A and human sc adipocytes. In insulin-sensitive adipocytes, ISO stimulated the 2-NBDG uptake by 210% (murine) and 67% (human), compared with insulin treatment. Notably, ISO also induced 2-NBDG uptake in murine (139%) and human (60%) adipocytes made resistant to insulin by treatment with TNF-␣, compared with the incorporation induced in these cells by rosiglitazone. ISO induction of glucose uptake in adipocytes was abolished by inhibitors of the insulin signaling pathway. These inhibitors also blocked the proper phosphorylation of insulin signaling pathway components induced by ISO in both insulin-sensitive and insulinresistant adipocytes. Additionally, ISO stimulated the transcription of genes encoding components of insulin signaling pathway in murine insulin-sensitive and insulin-resistant adipocytes. In summary, we show here that ISO exerts its antidiabetic effects by activating the insulin signaling pathway in adipocytes, reverts the insulin resistance caused in these cells by TNF-␣ by stimulating the proper phosphorylation of proteins in this signaling pathway, and induces the expression of genes encoding these proteins. (Endocrinology 153: 5222-5230, 2012)

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Section: Discussionsupporting

confidence: 64%

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

et al. 2012

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“…23) AlonsoCastro and coworkers also reported that honokiol increased glucose uptake in murine and human adipocytes by stimulating insulin signaling pathways. 24) Collectively, these findings and our results strongly suggest that honokiol can efficiently stimulate 3T3-L1 cell differentiation via multiple pathways, including RXR activation, resulting in enhanced insulin sensitivity or protection against obesity.…”

Section: Discussionsupporting

confidence: 68%

A Naturally Occurring Rexinoid, Honokiol, Can Serve as a Regulator of Various Retinoid X Receptor Heterodimers

Kotani

Tanabe

Mizukami

et al. 2012

Biological & Pharmaceutical Bulletin

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We investigated the properties of honokiol, a natural rexinoid, as a regulator of retinoid X receptor (RXR) heterodimers with various partner nuclear receptors (NRs), in comparison with those of the synthetic rexinoid bexarotene. Honokiol alone was hardly capable of activating peroxisome proliferator-activated receptor (PPAR) γ/RXR, RXR/liver X receptor (LXR), and RXR/vitamin D receptor (VDR) heterodimers, whereas it effectively potentiated their activation by agonists for the partner NRs of the RXR heterodimers. These findings were further supported by increased mRNA and protein levels for the respective NR target genes. Bexarotene alone activated PPARγ/RXR and RXR/LXR heterodimers, but not RXR/VDR heterodimers, and facilitated the activation of all three RXR heterodimers by the respective PPARγ, LXR, and VDR agonists. When the potencies of honokiol and bexarotene were compared, honokiol was able to serve as a subsidiary agonist in the activation of RXR heterodimers in a similar manner to bexarotene. However, it seemed to potentiate the activation of PPARγ/RXR heterodimers by the PPARγ agonist rosiglitazone more efficiently than bexarotene, and was a less potent RXR agonist than bexarotene. In conclusion, we have demonstrated that honokiol is a rexinoid that possesses distinct properties from bexarotene, and mainly has subsidiary roles in the activation of RXR heterodimers by potentiating the activation of RXR heterodimers by agonists for the partner NRs.

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“…DMSO at final concentration of 0.01% was used as vehicle control. After 48 h of treatment, the MTT assay was performed and the relative viability was calculated as described previously (Alonso-Castro et al, 2011). The concentration leading to 50% inhibition of viability (IC 50 ) was also calculated by regression analysis (percent survival versus log concentration).…”

Section: Cell Viability Assaymentioning

confidence: 99%

Antitumor effect of Croton lechleri Mull. Arg. (Euphorbiaceae)

Alonso-Castro

Ortíz‐Sánchez

Domínguez³

et al. 2012

Journal of Ethnopharmacology

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Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway

Cited by 58 publications

References 19 publications

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

A Naturally Occurring Rexinoid, Honokiol, Can Serve as a Regulator of Various Retinoid X Receptor Heterodimers

Antitumor effect of Croton lechleri Mull. Arg. (Euphorbiaceae)

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