Magnolol and honokiol regulate the calcium-activated potassium channels signaling pathway in Enterotoxigenic Escherichia coli-induced diarrhea mice

Deng, Yanli; Han, Xuefeng; Tang, Shaoxun; Xiao, Wenjun; Tan, Zhiliang; Zhou, Changlin; Wang, Min; Kang, Jian

doi:10.1016/j.ejphar.2015.03.002

Cited by 31 publications

(27 citation statements)

References 38 publications

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“…Magnolol is not only an anti-inflammatory agent but also a modulator on gastrointestinal motility, which is supported by the findings that: (1) magnolol suppresses pro-inflammatory cytokines and modulates the related pathways in vitro [ 23 , 24 , 25 ]; (2) magnolol exhibits significant therapeutic effects on animal models with different inflammatory diseases [ 11 , 13 , 14 , 26 ]; (3) magnolol exerts inhibitory effects on the smooth muscle contraction of gastric, ileum and colon [ 19 , 27 ]; (4) interestingly, magnolol shows stimulatory effects on gastrointestinal motility at lower dosages [ 22 , 28 ]; (5) magnolol regulates the calcium-activated potassium channels signaling pathway in the enterotoxigenic Escherichia coli-induced diarrhea mice [ 29 ]. These evidences indicate that magnolol have potential value in the treatment of gastroenterology disorders caused by intestinal inflammation or diarrhea such as colitis.…”

Section: Discussionmentioning

confidence: 99%

Magnolol, a Natural Polyphenol, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

Zhao

Xiao

et al. 2017

Molecules

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Magnolol is a lignan with anti-inflammatory activity identified in Magnolia officinalis. Ulcerative colitis (UC), one of the types of inflammatory bowel disease (IBD), is a disease that causes inflammation and ulcers in the colon. To investigate the effect of magnolol in dextran sulfate sodium (DSS)-induced experimental UC model, male C57 mice were treated with 2% DSS drinking water for 5 consecutive days followed by intragastric administration with magnolol (5, 10 and 15 mg/kg) daily for 7 days. The results showed that magnolol significantly attenuated disease activity index, inhibited colonic shortening, reduced colonic lesions and suppressed myeloperoxidase (MPO) activity. Moreover, colonic pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) induced by colitis were dramatically decreased by magnolol. To further unveil the metabolic signatures upon magnolol treatment, mass spectrometry-based metabolomic analysis of the small molecular metabolites in mice serum were performed. Compared with controls, abnormality of serum metabolic phenotypes in DSS-treated mice were effectively reversed by different doses of magnolol. In particular, magnolol treatment effectively elevated the serum levels of tryptophan metabolites including kynurenic acid (KA), 5-hydroxyindoleacetic acid, indoleacetic acid (IAA), indolelactic acid and indoxylsulfuric acid, which are potential aryl hydrocarbon receptor (AHR) ligands to impact colitis. These findings suggest that magnolol exerts anti-inflammatory effect on DSS-induced colitis and its underlying mechanisms are associated with the restoring of tryptophan metabolites that inhibit the colonic inflammation.

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Section: Discussionmentioning

confidence: 99%

Magnolol, a Natural Polyphenol, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

Zhao

Xiao

et al. 2017

Molecules

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“…The commercial magnolol power (extracted from the cortex of Magnolia officinalis, purity > 98.0 % in dry) was purchased from Jinnong Bio Co. Ltd. (Changsha, China) and kept at -20°C. The ETEC O 78 :K 80 (44813) suspensions (3.45×109 CFU/ml) was prepared in accordance with our previous report [44], and cultured overnight in lysogeny broth (LB) medium in a shaking incubator at 180 rpm and 37°C for 12 h. The cell density of ETEC suspension was measured by ND-1000 UV-vis spectrophotometer (Nano-Drop Ltd., TX).…”

Section: Extracts and Reagentsmentioning

confidence: 99%

“…The commercial magnolol power was equally dissolved in 0.4 ml 2% Tween 80 solution before oral administration by gavage for each mouse. The ETEC suspension was prepared according to our previous report [44], and the density was measured with ND-1000 UV-vis spectrophotometer (NanoDrop Ltd., TX) at optical density 600 (OD 600=1 represents 1 × 10 10 CFU/ml cell density). In the present study, the determined cell density of ETEC suspension was 3.45×10 9 CFU/ml.…”

Section: Experimental Designmentioning

confidence: 99%

“…Total RNA from the mouse livers was isolated using TRIzol reagent (Invitrogen, CA, USA) ∆according to the manufacturer's instructions within minor modification [44]. The concentration of isolated total RNA was quantified with an ND-1000 UV-vis spectrophotometer.…”

Section: Real-time Quantitative Rt-pcrmentioning

confidence: 99%

“…The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize target gene transcript levels. Real-time PCR was performed with SYBR R Premix Ex Tap TM II (Tli RNaseH Plus) kits according to the manufacturer's instructions [44]. Briefly, 1 μl cDNA template was added to a total volume of 10 μl containing 5 μl SYBR Green mix, 0.2 μl Rox, 3 μl DEPC-treated distilled water, and 0.4 μl each of the forward and reverse primers (10 nM).…”

Section: Real-time Quantitative Rt-pcrmentioning

confidence: 99%

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General Machine Learning Model, Review, and Experimental-Theoretic Study of Magnolol Activity in Enterotoxigenic Induced Oxidative Stress

Deng

Liu

Tang

et al. 2017

CTMC

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This study evaluated the antioxidative effects of magnolol based on the mouse model induced by Enterotoxigenic Escherichia coli (E. coli, ETEC). All experimental mice were equally treated with ETEC suspensions (3.45×109 CFU/ml) after oral administration of magnolol for 7 days at the dose of 0, 100, 300 and 500 mg/kg Body Weight (BW), respectively. The oxidative metabolites and antioxidases for each sample (organism of mouse) were determined: Malondialdehyde (MDA), Nitric Oxide (NO), Glutathione (GSH), Myeloperoxidase (MPO), Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx). In addition, we also determined the corresponding mRNA expressions of CAT, SOD and GPx as well as the Total Antioxidant Capacity (T-AOC). The experiment was completed with a theoretical study that predicts a series of 79 ChEMBL activities of magnolol with 47 proteins in 18 organisms using a Quantitative Structure- Activity Relationship (QSAR) classifier based on the Moving Averages (MAs) of Rcpi descriptors in three types of experimental conditions (biological activity with specific units, protein target and organisms). Six Machine Learning methods from Weka software were tested and the best QSAR classification model was provided by Random Forest with True Positive Rate (TPR) of 0.701 and Area under Receiver Operating Characteristic (AUROC) of 0.790 (test subset, 10-fold crossvalidation). The model is predicting if the new ChEMBL activities are greater or lower than the average values for the magnolol targets in different organisms.

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Discrimination between raw and ginger juice processed Magnoliae officinalis cortex based on HPLC and Heracles NEO ultra‐fast gas phase electronic nose

Zhang

Wang

Fan

et al. 2022

Phytochemical Analysis

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Introduction: Magnoliae officinalis cortex (MOC), a traditional Chinese medicine, has been used in treating gastrointestinal diseases since ancient time. According to the Chinese Pharmacopoeia, it includes two kinds of decoction pieces, raw and ginger juice processed Magnoliae officinalis cortex (RMOC and GMOC).Objective: The aim of this paper was to study the differences between non-volatile and volatile components in RMOC and GMOC. Methods:The non-volatile components were detected by HPLC fingerprinting coupled with content determination (syringin, magnoflorine, honokiol and magnolol).Meanwhile, their odor information was obtained using a Heracles NEO ultra-fast gas phase electronic nose to conduct radar fingerprint analysis, principal component analysis and discriminant factor analysis, and the volatile components were analyzed qualitatively by the Kovats retention index and the AroChemBase database.Results: The HPLC fingerprints were established and 20 common peaks were found in all chromatograms with similarity values of more than 0.900. The content determination results showed that the contents of syringin and magnoflorine decreased, while the contents of honokiol and magnolol increased in GMOC. By the gas phase electronic nose, the two decoction pieces could be distinguished obviously and 16 possible compounds were identified. Among them, the relative contents of (À)-α-pinene and β-pinene increased, while β-phellandrene and (+)-limonene levels decreased. Conclusion:The results suggested that honokiol, magnolol, (À)-α-pinene and β-pinene might be the main substances which could enhance the harmonizing effect on the stomach. Moreover, this paper could lay a foundation for exploring the processing mechanism of MOC and provide a novel method for the research of other traditional Chinese medicine with strong aroma.

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Magnolol and honokiol regulate the calcium-activated potassium channels signaling pathway in Enterotoxigenic Escherichia coli-induced diarrhea mice

Cited by 31 publications

References 38 publications

Magnolol, a Natural Polyphenol, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

Magnolol, a Natural Polyphenol, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

General Machine Learning Model, Review, and Experimental-Theoretic Study of Magnolol Activity in Enterotoxigenic Induced Oxidative Stress

Discrimination between raw and ginger juice processed Magnoliae officinalis cortex based on HPLC and Heracles NEO ultra‐fast gas phase electronic nose

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