Magnolol Suppresses MetastasisviaInhibition of Invasion, Migration, and Matrix Metalloproteinase-2/-9 Activities in PC-3 Human Prostate Carcinoma Cells

Magnolol-Induced Apoptosis in HCT-116 Colon Cancer Cells Is Associated with the AMP-Activated Protein Kinase Signaling Pathway

“…18,19) Several reports have demonstrated that magnolol treatment does not affect the viability of normal cells such as human prostate epithelial cells and normal blood cells. 18,25) We also observed that magnolol showed no toxicity (<50 µM) in fibroblasts (NIH-3T3) (data not shown).…”

“…43,45) Magnolol suppresses metastasis by inhibiting invasion, migration, and matrix metalloproteinase activities in PC-3 and HT1080 cells. 19) The antimetastatic effects of magnolol in an experimental liver and spleen metastasis model using L5178YML25 lymphoma and in an experimental and spontaneous lung metastasis model of B16-BL6 melanoma, have been evaluated. 46) In this study, we determined the antimetastatic effect of magnolol via AMPK activation using wound migration and invasion assays.…”

“…18) Attention has been paid to its anticancer activity in several cancer cell lines, including colon cancer, hepatoma, leukemia, fibrosarcoma, melanoma, squamous carcinoma, and thyroid carcinoma. 18,19) Several researchers have shown that magnolol inhibits proliferation and induces apoptosis in cancer cells by upregulating p21 cip/Waf1 , 20) inhibiting DNA synthesis, 21) activating the epidermal growth factor receptor (EGFR)/phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, 18) as well as Ras/Raf-1/ Erk actions. 22) However, it remains to be determined whether Note * To whom correspondence should be addressed.…”

Mere15, a novel polypeptide fromMeretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs

“…The cell adhesion assay was performed as described by Hwang and Park (2010). The 96-well tissue culture plates were coated with 50.0 μg/well of extracellular matrix (ECM) or fibronectin (FN) (Sigma-Aldrich, St. Louis, MO, USA).…”

“…PCR was carried out in 50 μL of reaction mixture containing 25 μL Premix Taq DNA polymerase buffer, 0.5 μL of each primer (20 μM), and 2 μL cDNA. The primers used in this study referred to Hwang et al (Hwang & Park, 2010): MMP-2, forward primer, 5′-GGCCCTGTCACTCCTGAGAT-3′, reverse primer, 5′-GGCATCCAGGTTATCGGGGA-3′; MMP-9, forward primer, 5′-CGGAGCACGGAGACGGGTAT-3′, reverse primer, 5′-TGAAGGGGAAGACGCACAGC-3′; GADPH, forward primer, 5′-GAGTCCACTGGCGTCTTCAC-3′, reverse primer, 5′-GTTCACACCCATGACGAACA-3′. PCR was performed under the following conditions: an initial denaturation step at 95°C for 3 min, followed by 35 cycles at 94°C for 30 s, 58°C for 45 s, and 72°C for 1 min and a final extension step at 72°C for 10 min.…”