Main-chain degradable single-chain cyclized polymers as gene delivery vectors

Gao, Yongsheng; Böhmer, Verena I.; Zhou, Daohong; Zhao, Tianyu; Wang, Wenxian; Paulusse, Jos M. J.

doi:10.1016/j.jconrel.2016.07.046

Cited by 34 publications

(31 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, PEI is the most commonly used cationic materials and can been seen as the gold standard for loading generic drugs. However, the greater toxicity of PEI has always been problematic [ 34 ]. In this study, we linked the PEI with PLGA to form PLGA-PEI, which reduced the toxicity of PEI but did not affect its ability to carry generic drugs [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a PSMA-Targeted Nanosystem Co-Packaging Docetaxel and Androgen Receptor siRNA for Castration-Resistant Prostate Cancer Treatment

et al. 2022

View full text Add to dashboard Cite

Primary prostate cancer (PC) progresses to castration-resistant PC (CRPC) during androgen deprivation therapy (ADR) in early stages of prostate cancer. Thus, rather than blocking the androgen-related pathway further, docetaxel (DTX)-based therapy has become the most effective and standard first-line chemotherapy for CRPC. Although the therapy is successful in prolonging the survival of patients with CRPC, chemotherapy resistance develops due to the abnormal activation of the androgen receptor (AR) signaling pathway. Thus, to optimize DTX efficacy, continued maximum suppression of androgen levels and AR signaling is required. Here, we designed a prostate-specific membrane antigen (PSMA)-targeted nanosystem to carry both DTX and AR siRNA (Di-PP/AR-siRNA/DTX) for CRPC treatment. Specifically, DTX was encapsulated into the hydrophobic inner layer, and the AR siRNA was then condensed with the cationic PEI block in the hydrophilic outer layer of the PEI-PLGA polymeric micelles. The micelles were further coated with PSMA-targeted anionic polyethylene glycol-polyaspartic acid (Di-PEG-PLD). In vitro and in vivo results demonstrated that the resulting Di-PP/AR-siRNA/DTX exhibited prolonged blood circulation, selective targeting, and enhanced antitumor effects. Consequently, Di-PP/AR-siRNA/DTX holds great potential for efficient CRPC treatment by combining chemotherapy and siRNA silencing of androgen-related signaling pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a PSMA-Targeted Nanosystem Co-Packaging Docetaxel and Androgen Receptor siRNA for Castration-Resistant Prostate Cancer Treatment

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Paulusse and Wang combined the use of single chain cyclized/knotted nanoparticles 209 (SCKNP) and SCM monomers to prepare gene delivery cargos that could degrade under reductive conditions. 312 The RAFT polymerization under kinetic control of DAEMA and various diacrylates (TEGDA or DSDA see Figure 116) in presence of 5 % of SCM7 (Figure 33), led to SCKNP of 10 kDa that could be degraded under reductive conditions (hydrazine hydrate or glutathione). The degradation experiments confirmed both the single chain nature of the nanoparticles and the degradation of the backbone (Figure 116).…”

Section: Biomaterials For Gene/dna Transfectionmentioning

confidence: 99%

Radical Ring-Opening Polymerization: Scope, Limitations, and Application to (Bio)Degradable Materials

et al. 2017

View full text Add to dashboard Cite

Cyclic monomers bearing either vinyl or exomethylene groups have the ability to be polymerized through a radical pathway via a ring-opening mechanism (addition-fragmentation process), leading to the introduction of functionalities in the polymer backbone. Radical ring-opening polymerization (rROP) combines the advantages of both ring-opening polymerization and radical polymerization, that is the preparation of polymers bearing heteroatoms in the backbone but with the ease and robustness of a radical process. This current review presents a comprehensive description of rROP by detailing: (i) the various monomers that polymerize through rROP; (ii) the main parameters that govern the rROP mechanism; (iii) the copolymerization by conventional or controlled/living radical polymerization between rROP monomers and traditional vinyl monomers to obtain copolymers with advanced properties; (iv) the different applications (low shrinkage materials and preparation of (bio)degradable materials) of rROP monomer-containing materials, and (v) the main alternatives to rROP to induce degradability to materials obtained by a radical polymerization.

show abstract

“…[56] In their study, readily available vinyl monomers, such as EGDMA, 2-(dimethylaminoethyl) methacrylate (DMAEMA), and polyethylene glycol methyl ether methacrylate (PEGMEMA), were copolymerized by a one-step DE-ATRP to synthesize cyclized single-chain cationic polymers with high molecular weights (> 20 kDa; Figure 9). [73] Besides these single-knot polymers, Aied et al applied a multiknot-structured polymer for the correction of collagen type VII-null skin cells of patients with recessive dystrophic epidermolysis bullosa (RDEB). The cyclic polymers were found to be superior to the dendrimeric commercial agent SuperFect, a result that was attributed to the special interaction between cyclic polymers and plasmid DNA.…”

Section: Applicationsmentioning

confidence: 99%

“…[58,60] A main-chain-reducible cyclized/knotted single-chain polymer for gene delivery was further developed by combining ring-opening addition polymerization and intramolecular cyclization into a one-pot RAFT copolymerization of cyclic allylsulfide and mono/multivinyl monomers. [73] Besides these single-knot polymers, Aied et al applied a multiknot-structured polymer for the correction of collagen type VII-null skin cells of patients with recessive dystrophic epidermolysis bullosa (RDEB). [59] Their findings suggested that the high efficiency stems from both the dense multiknot architecture and its degradable properties, which facilitate both the binding and release of the plasmid DNA.…”

Section: Applicationsmentioning

confidence: 99%

Controlled Polymerization of Multivinyl Monomers: Formation of Cyclized/Knotted Single‐Chain Polymer Architectures

et al. 2016

Self Cite

View full text Add to dashboard Cite

Seventy years ago, Flory and Stockmayer predicted that the polymerization of multivinyl monomers (MVMs) would inevitably lead to insoluble cross-linked gel networks. Since then, the use of MVMs has largely been limited to as cross-linking agents. More recently, however, polymerization strategies such as reversible deactivation radical polymerization (RDRP) have paved the way for the exploration of new possibilities in terms of both polymer architectures and functional capabilities. This Minireview provides historical context to the problem of polymerizing MVMs, before highlighting how RDRP has led to the formation of new cyclized/knotted polymer structures. Although the potential of such cyclized/knot polymer architectures is far from being fulfilled, some emerging applications are discussed.

show abstract

Main-chain degradable single-chain cyclized polymers as gene delivery vectors

Cited by 34 publications

References 71 publications

Development and Evaluation of a PSMA-Targeted Nanosystem Co-Packaging Docetaxel and Androgen Receptor siRNA for Castration-Resistant Prostate Cancer Treatment

Development and Evaluation of a PSMA-Targeted Nanosystem Co-Packaging Docetaxel and Androgen Receptor siRNA for Castration-Resistant Prostate Cancer Treatment

Radical Ring-Opening Polymerization: Scope, Limitations, and Application to (Bio)Degradable Materials

Controlled Polymerization of Multivinyl Monomers: Formation of Cyclized/Knotted Single‐Chain Polymer Architectures

Contact Info

Product

Resources

About