Main trends in the genetic transformation of Populus species

Kutsokon, N. K.

doi:10.3103/s009545271106003x

Cited by 8 publications

(4 citation statements)

References 57 publications

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“…All tests were two-tailed, considering P values < 0.05 as significant. Both allelic and genotypic associations between single SNPs and RA susceptibility were analyzed using 2 tests [21]. Results.…”

Section: Abstract: Rheumatoid Arthritis Toll Like Receptors Gene mentioning

confidence: 99%

The relationship between SNPS in the genes of TLR signal transduction pathway downstream elements and rheumatoid arthritis susceptibility

Liu

et al. 2014

Cytol. Genet.

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Section: Abstract: Rheumatoid Arthritis Toll Like Receptors Gene mentioning

confidence: 99%

The relationship between SNPS in the genes of TLR signal transduction pathway downstream elements and rheumatoid arthritis susceptibility

Liu

et al. 2014

Cytol. Genet.

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“…In vitro shoot regeneration is not only a rapid and reliable method for valuable genotypes propagation but also the indispensable prerequisite for some biotechnological techniques, such as genetic transformation and gene editing. Genetic manipulations are able to increase the plant growth and resistance to biotic and abiotic stresses, change the quality of wood (e.g., reduce or change lignin content), and improve phytoremediation characteristics (Confalonieri et al 2003;Kutsokon 2011;Song et al 2019). However, not all tree species can be easily in vitro regenerated.…”

Section: Introductionmentioning

confidence: 99%

In vitro culture initiation and regeneration of two highly productive clones of poplar

Khoma

Khudolieieva

Rashydov

et al. 2022

Nova Biotechnol. Chim.

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In vitro shoot regeneration is a fast and reliable method for propagation of valuable tree genotypes and important step in genetic manipulations. This study aimed to establish an optimal in vitro culture initiation and regeneration protocol for highly productive poplar clones ‘Novoberlinska-3’ (Populus pyramidalis × P. laurifolia) and ‘Volosystoplidna’ (P. trichocarpa). In vitro culture was initiated either on MS (IM-MS) or WPM (IM-WPM) medium both containing BAP (0.2 mg.L-1) and IBA (0.1 mg.L-1). Results demonstrated that the best initiation medium for the clone ‘Volosystoplidna’ was IM-WPM on which 93.3 % of explants survived, while explants from ‘Novoberlinska-3’ better survived on IM-MS. After establishing both genotypes into aseptic culture, regeneration experiments were started using two types of explants, leaf and petioles, planted on callus induction medium containing 2iP (1.02 mg.L-1) and NAA (1.86 mg.L-1). Furthermore, microshoots were placed on shoot induction medium supplemented with 0.04 mg.L-1 thidiazuron, and then transferred on shoot elongation medium with 0.2 mg.L-1 BAP. Regeneration protocol showed better performance of poplar ‘Novoberlinska-3’, but it was also efficient for ‘Volosystoplidna’. Plant regeneration from leaf explants in the clone ‘Novoberlinska-3’ showed the highest regeneration percentage (92.3 %) and the number of shoots per explant (3.1), which significantly exceeded other explants. Our results showed a significant difference between the survivability of two clones during culture initiation. The differences in regeneration rates between the clones as well as leaf and petiole explants were also determined. Obtained aseptic cultures of highly productive poplar hybrids will be used in our further studies, especially for genetic transformation.

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“…Additional characteristics of bioenergy trees may be drought tolerance, resistance to pests and insects, the ability to produce high biomass yields on different types of land (Sannigrahi et al 2010), tolerance to abiotic stresses and ability to phytoremediation. Some of the above-mentioned characteristics can be enhanced by genetic modifications (Kutsokon 2011).…”

Section: Introductionmentioning

confidence: 99%

Nursery screening of poplar and willow clones for biofuel application in Ukraine

Kutsokon¹,

Rakhmetov²,

Rakhmetova³

et al. 2022

iForest

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Biogeosciences and Forestry Biogeosciences and Forestry Nursery screening of poplar and willow clones for biofuel application in UkraineNataliia Kutsokon (1) , Dzhamal Rakhmetov (2) , Svitlana Rakhmetova (2) , Lidiia Khudolieieva (1) , Namik Rashydov (1) Poplars and willows are fast-growing trees that can be effectively grown as a renewable energy source. This study was devoted to the preliminary screening of poplar and willow clones for biofuel application in a fast-growing tree nursery established in the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine. The nursery includes 19 Populus and 10 Salix clones, with many hybrids of Ukrainian origin. The clones were assessed in the first two years in the nursery using growth parameters, biomass fuel criteria, and susceptibility to pathogens. Using total rank for evaluation, the highest rank was found in the poplar clone "Kanadska × balsamichna" followed by the clones "Ivantiivska", "Volosystoplidna", "Perspektyvna", and "Nocturn". Among the willows, the highest rank was recorded for the clone "Zhytomyrska-1", followed by clone "Zhytomyrska-2". High ranks were also found in the poplars "Strilopodibna", "Mobilna", "Novoberlinska-7" and "Keliberdynska", and the willows "Lisova pisnya" and "Vinnytska". Thus, the above-mentioned clones may be recommended as promising trees, though they should be further evaluated under field conditions for growth performance within the short-rotation cycles. The clones with the lowest total rank were poplars "Bolle", "Gradizka" and "Kytaiska × piramidalna" and willows "Lukash", "Olimpiisky vohon" and "Pryberezhna" are not recommended for bioenergy short-rotation plantations. Evaluation of plants in the nursery allowed us to carry out rapid and cost-effective preliminary screening. Such multiclonal screening of bioenergy trees for planting in short rotations was described for the first time in Ukraine.

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Main trends in the genetic transformation of Populus species

Cited by 8 publications

References 57 publications

The relationship between SNPS in the genes of TLR signal transduction pathway downstream elements and rheumatoid arthritis susceptibility

The relationship between SNPS in the genes of TLR signal transduction pathway downstream elements and rheumatoid arthritis susceptibility

In vitro culture initiation and regeneration of two highly productive clones of poplar

Nursery screening of poplar and willow clones for biofuel application in Ukraine

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