Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

Arenas, Claudia Marcela; Gómez-Molina, Andrea; Delgado, Jean Paul

doi:10.1007/978-1-4939-2495-0_5

Cited by 6 publications

(5 citation statements)

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“…Wild adult salamanders (7–10 cm snout to tail length) of the species Bolitoglossa ramosi were collected from their type locality by the nocturnal visual encounter method [ 19 ] in the Andes region of Antioquia, Colombia. Specimens were kept in the laboratory under established protocols [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research

et al. 2018

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BackgroundTissue regeneration is widely distributed across the tree of life. Among vertebrates, salamanders possess an exceptional ability to regenerate amputated limbs and other complex structures. Thus far, molecular insights about limb regeneration have come from a relatively limited number of species from two closely related salamander families. To gain a broader perspective on the molecular basis of limb regeneration and enhance the molecular toolkit of an emerging plethodontid salamander (Bolitoglossa ramosi), we used RNA-Seq to generate a de novo reference transcriptome and identify differentially expressed genes during limb regeneration.ResultsUsing paired-end Illumina sequencing technology and Trinity assembly, a total of 433,809 transcripts were recovered and we obtained functional annotation for 142,926 non-redundant transcripts of the B. ramosi de novo reference transcriptome. Among the annotated transcripts, 602 genes were identified as differentially expressed during limb regeneration. This list was further processed to identify a core set of genes that exhibit conserved expression changes between B. ramosi and the Mexican axolotl (Ambystoma mexicanum), and presumably their common ancestor from approximately 180 million years ago.ConclusionsWe identified genes from B. ramosi that are differentially expressed during limb regeneration, including multiple conserved protein-coding genes and possible putative species-specific genes. Comparative analyses reveal a subset of genes that show similar patterns of expression with ambystomatid species, which highlights the importance of developing comparative gene expression data for studies of limb regeneration among salamanders.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5076-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

“…1b ). Briefly, animals were anesthetized by immersion in 1% Tricaine (SIGMA, USA) before bilateral-proximal amputations of the forelimb were performed at mid-humerus level using microscissors and forceps (Fine Precision Tools, USA) [ 20 ]. The protruding bone and muscle were trimmed to produce a flat wound surface.…”

Section: Methodsmentioning

confidence: 99%

Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research

et al. 2018

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“…In our lab, we developed a protocol to keep bolitoglossines in captivity during a short time period (Arenas Gómez et al, 2015). This will make them available to understand how limb regeneration works in species that belong to the Plethodontidae family.…”

Section: The Colombian Experience: Limb Regeneration In Bolitoglossa ...mentioning

confidence: 99%

Limb regeneration in salamanders: the plethodontid tale

Gómez¹,

Delgado²

2021

Int. J. Dev. Biol.

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Salamanders are the only vertebrates that can regenerate limbs as adults. This makes them ideal models to investigate cellular and molecular mechanisms of tissue regeneration. Ambystoma mexicanum and Nothopthalmus viridescens have long served as primary salamander models of limb regeneration, and the recent sequencing of the axolotl genome now provides a blueprint to mine regeneration insights from other salamander species. In particular, there is a need to study South American plethodontid salamanders that present different patterns of limb development and regeneration. A broader sampling of species using next-generation sequencing approaches is needed to reveal shared and unique mechanisms of regeneration, and more generally, the evolutionary history of salamander limb regeneration.

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“…In the field of regenerative biology, amphibians represent one of the key groups of animals in which to study tissue regeneration. Although representative studies in this area have been carried out in our country, there is a clear need to continue expanding our knowledge in this discipline (Arenas et al, 2015). Therefore, the implementation of animal models for research in developmental and regenerative biology is a task that must be carried out.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Also, morphological, histological and molecular aspects of limb regeneration in the wild species Bolitoglossa ramosi have been described ( Arenas Gómez et al, 2017;Arenas Gómez et al, 2018), aiming to compare it with traditional model salamanders of the families Ambystomidae and Salamandridae and eventually, to establish B. ramosi as a new model organism for regeneration, to understand how conserved limb regeneration is between salamander families. Husbandry of terrestrial plethodontid salamanders in a laboratory environment have been attempted with B. ramosi and Bolitoglossa vallecula in an attempt to use them as models in regeneration (Arenas Gómez et al, 2015); however, these species require specific conditions for reproduction which have to be elucidated. Currently, the difficulty to reproduce these species in a laboratory environment constitutes the main barrier to use them as model organisms in regeneration.…”

Section: Perspectives For Ambystoma Mexicanum As a Model Organism In Colombiamentioning

confidence: 99%

Ambystoma mexicanum, a model organism in developmental biology and regeneration: a colombian experience

Agudelo

Medina

Gómez³

2021

Acta biol. Colomb.

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Ambystoma mexicanum is a urodele amphibian endemic to Xochimilco Lake in Mexico, it belongs to the salamander family Ambystomatidae. This species has frequently been used as model organism in developmental biology and regeneration laboratories around the world due to its broad regenerative capacities and adaptability to laboratory conditions. In this review we describe the establishment of the first colony of axolotls in Colombia to study tissue regeneration and our perspectives on the use A. mexicanum as a model organism in Colombia are discussed emphasizing its possible uses in regeneration and developmental biology

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Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

Cited by 6 publications

References 12 publications

Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research

Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research

Limb regeneration in salamanders: the plethodontid tale

Ambystoma mexicanum, a model organism in developmental biology and regeneration: a colombian experience

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