1999
DOI: 10.1111/j.1469-7793.1999.0507t.x
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Maintenance and regulation of the pH microclimate at the luminal surface of the distal colon of guinea‐pig

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Cited by 51 publications
(47 citation statements)
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“…To examine directly whether the cAMP pathway stimulated V-ATPase-dependent proton transport across the apical membrane, luminal pH changes were determined by ratiometric fluorescence measurements on isolated salivary glands with 5-N-hexadecanoyl-aminofluorescein (HAF) injected into their lumen. The palmitoyl chain of this molecule inserts into the outer leaflet of the plasma membrane, and its pH-sensitive fluorescent moiety is then exposed on the luminal surface (32). Superfusion of the glands with a saturating concentration of 5-HT led to a reversible, monophasic luminal acidification, as indicated by the decrease in the fluorescence ratio (Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To examine directly whether the cAMP pathway stimulated V-ATPase-dependent proton transport across the apical membrane, luminal pH changes were determined by ratiometric fluorescence measurements on isolated salivary glands with 5-N-hexadecanoyl-aminofluorescein (HAF) injected into their lumen. The palmitoyl chain of this molecule inserts into the outer leaflet of the plasma membrane, and its pH-sensitive fluorescent moiety is then exposed on the luminal surface (32). Superfusion of the glands with a saturating concentration of 5-HT led to a reversible, monophasic luminal acidification, as indicated by the decrease in the fluorescence ratio (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…For monitoring pH changes at the luminal surface of the secretory cells, we adapted a microf luorometric method that was previously used on guinea pig colonic epithelium (32). The fatty acyl chain of this dye integrates into the outer leaf let of the plasma membrane, and its pH-sensitive f luorescent moiety faces then the extracellular space (32). Salivary glands were attached to the Cell-Tak-coated (BD Biosciences, San Jose, CA) surface of a glass-bottom perfusion chamber that was then placed on a Zeiss Axiovert 135 inverted microscope.…”
Section: Methodsmentioning
confidence: 99%
“…The pH of the luminal contents varies along the length of the gastrointestinal (GI) tract (Genz et al, 1999). In the transitional region encompassing the distal small intestine, caecum and proximal large intestine, the pH abruptly changes from near neutral to acidic, reflecting the increase in the density of the resident caecal bacteria and the amounts of SCFAs they produce.…”
Section: Discussionmentioning
confidence: 99%
“…Whether the trophozoites contribute to production of the un-ionised SCFA fraction by local release of H+ via Na+-H+ exchange, as colonic epithelial cells do (Genz et al, 1999;Schroder et al, 2000), has not yet been determined.…”
Section: Discussionmentioning
confidence: 99%
“…This creates a relatively constant microenvironment for the cells with changes in the narrow pH range of 7-7.5 (32,33). Hence, under physiological conditions the pH changes that occur at the apical surface of colonocytes regulate Zn 2ϩ -dependent intracellular signaling via ZnR/GPR39.…”
Section: Discussionmentioning
confidence: 99%