Maintenance of Escherichia coli and the Assimilation of Glucose

McGrew, Sarah B.; Mallette, M. F.

doi:10.1038/2081096a0

Cited by 10 publications

(4 citation statements)

References 3 publications

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“…The amounts of C14 assimilated in these suspensions of K-12 were lower than those in short-term experiments with glucose-grown cells (2) and tended to diminish with time of aging, as also observed by McGrew and Mallette (7). The lower extent of assimilation probably reflects endogenous oxidation of assimilated carbon.…”

Section: Resultssupporting

confidence: 64%

“…Mallette (8) and McGrew and Mallette (7) reported higher percentages of survivors in their suspensions of E. coli fed glucose than we observed. They also observed increases in turbidity after repeated additions of appropriate amounts of glucose, whereas we noted only decreases in turbidity; these decreases were, however, somewhat less in the fed than in the nonfed suspensions.…”

Section: Resultscontrasting

confidence: 46%

“…The concept of energy of maintenance requirements has been supported by the results reported by Marr (11) and Mallette (7,8) and their coworkers from studies on the growth of E. coli (11) and on the influence of feeding on growth and viability (7,8). In the present study, the effects of feeding glucose to E. coli on both endogenous and exogenous respiration and on turbidity and viability were determined.…”

Section: Resultsmentioning

confidence: 51%

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Aging of Escherichia coli

Clifton

1966

J Bacteriol

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The rates of endogenous and exogenous (glucose) respiration decreased much more rapidly than did the viable count during the first 24 hr of aging of washed, C'4-labeled cells of Escherichia coli K-12 suspended in a basal salt medium devoid of ammonium salts. The rates of decrease of respiration and of death approached each other as the age of the cells increased, but death was not the only factor involved in decreased respiratory activity of the suspensions. The greatest decrease in cellular contents with aging was noted in the ribonucleic acid fraction, of which the ribose appeared to be oxidized, while uracil accumulated in the suspension medium. The viable count and respiratory activities remained higher in glucose-fed than in nonfed suspensions. Proline-labeled cells fed glucose tended to lose more of their proline and to convert more proline into C1402 than in unfed controls. On the other hand, uracil-labeled cells fed glucose retained more of the uracil than did nonfed cells, but glucose elicited some oxidation of uracil. An exogenous energy source such as glucose aided in the maintenance of a population, but it was not the only factor needed for such maintenance.

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Section: Resultssupporting

confidence: 64%

Section: Resultscontrasting

confidence: 46%

Section: Resultsmentioning

confidence: 51%

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Aging of Escherichia coli

Clifton

1966

J Bacteriol

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“…In contrast to the effect of carbon substrates on starving organisms discussed above however, there are several recent reports of decreased death rates resulting from the intermittent addition of small amounts of glucose to Escherichia coli starved in buffer containing Mg2+ (Clifton, 1966;McGrew & Mallette, 1962Mallette, 1963). However, the evaluation of some of these results is difficult since the experimental procedure did not preclude regrowth (see Dawes & Ribbons, 1964;Postgate, 1967).…”

Section: I)mentioning

confidence: 92%

Metabolism of Exogenous Arginine and Glucose by Starved Streptococcus Lactis in Relation to Survival

Thomas

Batt

1969

Journal of General Microbiology

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SUMMARYArginine or glucose accelerated death of Streptococcus lactis starved in phosphate buffer. Both substrates were metabolized at linear but different rates and the rate of ATP production from glucose metabolism was about 7-5 times that for arginine metabolism. Mg2+ reduced glucose-accelerated death and abolished arginine-accelerated death to prolong survival. When glucose was fed continuously to organisms starved in phosphate buffer containing Mg2+, survival was related to the rate of glucose addition and hence to the rate of metabolism. By feeding low concentrations of glucose, the viability of most of the population was extended from about I to 4 days. The maintenance energy requirement appeared to be in the region of 0.045 to 0.09 mg. glucose/mg. dry wt bacteria/hr. The glycolytic activity of starved organisms declined at varying rates depending on the starvation environment but could not be correlated directly with loss of viability. Exogenous arginine substantially reduced the lethal effect of adverse pH values.

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Dialysis fermentation. II. Kinetic analysis of salicylic acid production via cell growth and maintenance

Abbott

1970

Biotech & Bioengineering

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A kinetic analysis was made of the relationship between salicylate production from naphthalene and growth of Psezldomms fEuoresce7ts in semicontinuous dialysis culture. The specific rates both of product formation and growth initially were increased by the diffusional withdrawal of salicylate, but subsequently were reduced to low levels despite continued salicylate removal. Productivity and growth were correlated by the Luedeking-Piret equation in an initial nondialysis period and in the early stages of dialysis fermentation, when specific growth rates exceeded .005 hr-1. Below this level of growth at later stages of dialysis fermentation, the specific production rate was correlated only with total cell mass by a proportionality constant of .035 hr-l, which was attributed to maintenance metabolism. Maintenance accounted for about 847, of the total salicylate produced, while growth-associated metabolism accounted for the remainder.

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Maintenance of Escherichia coli and the Assimilation of Glucose

Cited by 10 publications

References 3 publications

Aging of Escherichia coli

Aging of Escherichia coli

Metabolism of Exogenous Arginine and Glucose by Starved Streptococcus Lactis in Relation to Survival

Dialysis fermentation. II. Kinetic analysis of salicylic acid production via cell growth and maintenance

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