Maintenance of meiotic crossover against reduced double-strand break formation in fission yeast lacking histone H2A.Z

Yamada, Tsuneo; Yamada, Shintaro; Ding, Da‐Qiao; Fujita, Yuzo; Takaya, Emi; Hiraoka, Yasushi; Murakami, Hiroshi; Ohta, Kunihiro

doi:10.1016/j.gene.2020.144615

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The 3-fold higher IS:IH ratio of HJs with Mug20-LacI than with ade6-3049 (Figure 6A ) may be related to the previously observed crossover invariance – more uniform crossover frequency than DSB frequency ( 53 ). The mechanism for invariance has been unknown, but DSBs at hotspots with high IS:IH ratio are more dependent on the histone variant H2A.Z than are DSBs in cold regions or at weak hotspots ( 18 , 81 ). It was proposed that H2A.Z promotes LinE binding to chromatin-bound cohesin; this view suggests that crossover invariance is directly related to LinE function and loading.…”

Section: Discussionmentioning

confidence: 99%

Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair

Hyppa

Cho

Nambiar

et al. 2021

Nucleic Acids Research

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During meiosis, DNA double-strand breaks (DSBs) are formed at high frequency at special chromosomal sites, called DSB hotspots, to generate crossovers that aid proper chromosome segregation. Multiple chromosomal features affect hotspot formation. In the fission yeast S. pombe the linear element proteins Rec25, Rec27 and Mug20 are hotspot determinants – they bind hotspots with high specificity and are necessary for nearly all DSBs at hotspots. To assess whether they are also sufficient for hotspot determination, we localized each linear element protein to a novel chromosomal site (ade6 with lacO substitutions) by fusion to the Escherichia coli LacI repressor. The Mug20-LacI plus lacO combination, but not the two separate lac elements, produced a strong ade6 DSB hotspot, comparable to strong endogenous DSB hotspots. This hotspot had unexpectedly low ade6 recombinant frequency and negligible DSB hotspot competition, although like endogenous hotspots it manifested DSB interference. We infer that linear element proteins must be properly placed by endogenous functions to impose hotspot competition and proper partner choice for DSB repair. Our results support and expand our previously proposed DSB hotspot-clustering model for local control of meiotic recombination.

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Section: Discussionmentioning

confidence: 99%

Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair

Hyppa

Cho

Nambiar

et al. 2021

Nucleic Acids Research

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“…H2A.Z, encoded by the HTZ1 gene in budding yeast, is a highly conserved variant of the canonical histone H2A; yeast cells lacking H2A.Z have a variety of defects in gene expression, DNA damage repair, and chromosome segregation ( Santisteban et al 2000 ; Meneghini et al 2003 ; Krogan et al 2004 ; Kalocsay et al 2009 ; reviewed in Billon and Cote 2013 ; Giaimo et al 2019 ). Fission yeast lacking H2A.Z are deficient in the formation of the double-strand DNA breaks that initiate meiotic recombination ( Yamada et al 2018 ), yet increases the frequency of crossovers at some loci ( Yamada et al 2020 ). No such defect in DNA break formation was observed in budding yeast cells lacking H2A.Z ( Gonzalez-Arranz et al 2018 ); however, htz1Δ/htz1 Δ diploids were observed to show reduced sporulation efficiency and spore viability, had defects in resumption of progression through meiosis following activation of the meiotic recombination checkpoint, and exhibited reduced chromosome movement in meiotic prophase ( Gonzalez-Arranz et al 2018 , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae

Chigweshe

MacQueen

Holmes

2022

G3 Genes|Genomes|Genetics

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Progression through meiosis is associated with significant reorganization of chromosome structure, regulated in part by changes in histones and chromatin. Prior studies observed defects in meiotic progression in yeast strains lacking the linker histone H1 or variant histone H2A.Z. To further define the contributions of these chromatin factors we have conducted genetic and cytological analysis of cells undergoing meiosis in the absence of H1 and H2A.Z. We find that a spore viability defect observed in strains lacking H2A.Z can be partially suppressed if cells also lack histone H1, while the combined loss of both H1 and H2A.Z is associated with elevated gene conversion events. Cytological analysis of Red1 and Rec8 staining patterns indicates that a subset of cells lacking H2A.Z fail to assemble a proper chromosome axis, and the staining pattern of the synaptonemal complex protein Zip1 in htz1Δ/ htz1Δ cells mimics that of cells deficient for Rec8-dependent meiotic cohesion. Our results suggest a role for H2A.Z in the establishment or maintenance of the meiotic chromosome axis, possibly by promoting efficient chromosome cohesion.

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Maintenance of meiotic crossover against reduced double-strand break formation in fission yeast lacking histone H2A.Z

Cited by 2 publications

References 25 publications

Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair

Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair

Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae

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