Maintenance of pregnancy in ovariectomized Mongolian gerbils (Meriones unguiculatus)

Kai, Osamu; Matsumoto, Naeko; Takinosawa, Miho; Sonoda, Yutaka

doi:10.1046/j.1344-3941.2002.t01-1-00046.x

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…1986). Pregnancy in gerbils, however, resulted in abortion after an ovariectomy on day 20 of pregnancy, and the abortion was rescued by administrations of exogenous P 4 (Kai et al . 2002).…”

Section: Discussionmentioning

confidence: 99%

In vitro progesterone production of corpus luteum in pregnant Mongolian gerbils (Meriones unguiculatus)

Etoh

Tanaka

Sonoda

et al. 2004

Animal Science Journal

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The present study was conducted to determine the in vitro progesterone (P4) production by Mongolian gerbil (Meriones unguiculatus) corpus luteum (CL) on days 9, 18 and 22 of pregnancy in comparison with rat CL on days 9 and 16. The culture medium of one CL per well of a 96‐well plate was changed after 4 h incubation and the CL were further cultured for 24 h. The P4 in serum and in the medium were measured by enzyme immunoassay system. Gerbil CL weight measured after 24 h incubation was at the same level for all 3 days of pregnancy and significantly different from that of the rat CL for the 2 days of pregnancy. The serum P4 level in gerbils was high in early pregnancy and decreased in advanced days of pregnancy. In contrast, the P4 in rats was higher in the latter half of pregnancy. In vitro P4 production by a single CL after 4 h incubation decreased significantly during the days of pregnancy in both the gerbil and the rat. P4 values produced by the gerbil CL for 24 h incubation were significantly higher than after 4 h incubation in all 3 days of pregnancy, however, there was no difference between the two incubation time groups in the rat. In vitro P4 production by CL correlated well with the serum P4 level on the 3 days of pregnancy in the gerbil. In the rat, however, the relationship was inversed between the two parameters. The correlation in this experiment suggested that CL is the sole source of P4 for the maintenance of the gerbil pregnancy, with the result of abortion after an ovariectomy on day 20 of pregnancy as described in our previous report.

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Section: Discussionmentioning

confidence: 99%

In vitro progesterone production of corpus luteum in pregnant Mongolian gerbils (Meriones unguiculatus)

Etoh

Tanaka

Sonoda

et al. 2004

Animal Science Journal

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“…Mongolian gerbils bred at this laboratory (Kai et al . 1995, 2002a,b) were maintained at 22 ± 3°C with lighting from 05.00 to 19.00 hours (14:10 L : D).…”

Section: Methodsmentioning

confidence: 99%

Partial sequence of Mongolian gerbil (Meriones unguiculatus) immunoglobulin gamma heavy chain constant region

Ukaji

Sumiyama

Kai

2011

Animal Science Journal

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We determined the sequence of the immunoglobulin gamma heavy-chain constant (IGHC) region of the Mongolian gerbil (Meriones unguiculatus). To isolate a part of the IGHC complementary DNA, we designed primers on the basis of highly conserved sequences in mouse, rat and hamster. The deduced IGHC is structurally similar to counterparts in other mammalian species and shows 84.6% identity to the IGHC of hamster IgG, 76.6% to rat IgG1, 83.3% to rat IgG2a, 78.1% to mouse IgG1, 81.8% to mouse IgG2a, 79.1% to mouse IgG2b and 79.2% to mouse IgG3 at the nucleotide level. The results suggest that gerbil IgG is closely related to hamster IgG and rat IgG2a.

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“…Mongolian gerbils bred at this laboratory [3][4][5] were maintained at 22 ± 3°C with lighting from 0500 to 1900 h (14 light: 10 dark). Six females of inbred strain gerbils, 14-52 weeks of age, were used in the experiment.…”

Section: Animalsmentioning

confidence: 99%

Sequence Determination of the Heavy-Chain Constant Region in Four Immunoglobulin Classes of Mongolian Gerbils (<i>Meriones unguiculatus</i>)

2012

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Abstract:We determined partial cDNA sequences of four immunoglobulin (Ig) classes-IgM, IgG1, IgE, and IgA-of Mongolian gerbil (Meriones unguiculatus). Each deduced Ig heavy-chain constant (IGHC) region-Cµ, Cγ1, Cε, and Cα-is structurally similar to its counterparts in the mouse and rat, and phylogenetic analysis suggests that the gerbil Igs are evolutionarily close to their counterparts. In spite of the high sequence homology to the other rodent Cγ sequences, the gerbil Cγ1 sequence differs from our previously reported Cγ2. This result indicates that the gerbil has at least two IgG subclasses. These four gerbil IGHC cDNA sequences will be useful for determining gerbil Ig isotypes and examining the expression of gerbil Ig mRNAs in response to parasitic and bacterial infections.

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Maintenance of pregnancy in ovariectomized Mongolian gerbils (Meriones unguiculatus)

Cited by 3 publications

References 25 publications

In vitro progesterone production of corpus luteum in pregnant Mongolian gerbils (Meriones unguiculatus)

In vitro progesterone production of corpus luteum in pregnant Mongolian gerbils (Meriones unguiculatus)

Partial sequence of Mongolian gerbil (Meriones unguiculatus) immunoglobulin gamma heavy chain constant region

Sequence Determination of the Heavy-Chain Constant Region in Four Immunoglobulin Classes of Mongolian Gerbils (<i>Meriones unguiculatus</i>)

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