1999
DOI: 10.1094/cchem.1999.76.5.668
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Maize Endosperm Proteins That Contribute to Endosperm Lysine Content

Abstract: Cereal Chem. 76(5):668-672Nonzein proteins were analyzed using SDS-PAGE to study their relationship to lysine level in mature endosperm of three isogenic lines and 29 inbred lines. The majority of nonzein proteins are glutelins that contain a relatively high lysine content. Trinitrobenzene-sulfonic acid was used to visualize high-lysine proteins on nitrocellulose membrane. Seven intense yellow bands (high-lysine proteins) were found in the glutelin fraction: 35, 43, 48, 52, 84, 92, and 100 kDa. These seven gl… Show more

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Cited by 20 publications
(17 citation statements)
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“…In the developing o2 endosperm, the high lysine levels (Silva & Arruda, 1979;Gaziola et al, 1999) coincide with the decreased synthesis of zein proteins (Sodek & Wilson, 1971;Yau et al, 1999) and with the lower activity of the bifunctional enzyme LOR/SDH (Brochetto-Braga et al, 1992;Gaziola et al, 1999). However, the activity of this bifunctional protein during the maturation process of the grain would degrade the lysine not incorporated into proteins, decreasing the soluble concentration even further (Galili, 1995).…”
Section: Resultsmentioning
confidence: 99%
“…In the developing o2 endosperm, the high lysine levels (Silva & Arruda, 1979;Gaziola et al, 1999) coincide with the decreased synthesis of zein proteins (Sodek & Wilson, 1971;Yau et al, 1999) and with the lower activity of the bifunctional enzyme LOR/SDH (Brochetto-Braga et al, 1992;Gaziola et al, 1999). However, the activity of this bifunctional protein during the maturation process of the grain would degrade the lysine not incorporated into proteins, decreasing the soluble concentration even further (Galili, 1995).…”
Section: Resultsmentioning
confidence: 99%
“…Masa is used in the production of tortillas. Nowadays, table tortillas are highly popular in United States and, to some extent, also in Canada and some European countries [1]. The expanded consumption of tortillas has led to industrialization of the nixtamalization process for dry masa flour (DMF) production.…”
Section: Introductionmentioning
confidence: 99%
“…Estes resultados podem ter ocorrido em razão de mudanças nas proporções das proteínas acumuladas nos grãos, genericamente denominadas de zeínas e não-zeinas (albuminas, globulinas e glutelinas). Sabe-se que o aumento do conteúdo de proteína observado em decorrência da adubação nitrogenada resulta em diminuição da participação das zeínas, localizadas predominantemente no endosperma e que, ao contrário das não-zeínas, são deficientes em alguns aminoácidos, como lisina e triptofano (Yau et al, 1999). Sendo assim, ao mesmo tempo em que é esperado aumento linear do conteúdo de aminoácidos com o acúmulo das diferentes proteínas, também é razoável admitir que qualquer alteração no tipo de proteína depositada no grão contribua para a dificuldade na obtenção de relações mais estreitas entre aminoácidos e proteína bruta.…”
Section: Variávelunclassified