1990
DOI: 10.1073/pnas.87.1.46
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Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA.

Abstract: The opaque-2 locus (o2) in maize regulates the expression of many members of the zein multigene family of storage proteins. cDNA clones for a wild-type allele of the (o2) locus (02) were isolated from a maize endosperm cDNA library and sequenced. We found a 258-nucleotide 5' leader sequence containing three short open reading frames followed by a sequence specifying a protein of 437 amino acids. The presumptive amino acid sequence of the protein (02) specified by the 02 cDNA contains a "leucine-zipper" domain … Show more

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Cited by 415 publications
(374 citation statements)
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“…The expression of 0 2 in Escherichia coli was obtained by cloning the 0 2 cDNA (Schmidt et al, 1990) into the pET3c vector (Studier et al, 1990). A synthetic oligonucleotide was used to introduce an Ndel site at the ATG of the 0 2 cDNA clone by polymerase chain reaction.…”
Section: Plant Materials and Protein Extract Preparationmentioning
confidence: 99%
See 1 more Smart Citation
“…The expression of 0 2 in Escherichia coli was obtained by cloning the 0 2 cDNA (Schmidt et al, 1990) into the pET3c vector (Studier et al, 1990). A synthetic oligonucleotide was used to introduce an Ndel site at the ATG of the 0 2 cDNA clone by polymerase chain reaction.…”
Section: Plant Materials and Protein Extract Preparationmentioning
confidence: 99%
“…NaF (50 mM), ammonium molybdate (0.2 mM), ammonium metavanadate (5 mM), p-nitrophenyl phosphate (5 mM), and EGTA (1 mM) were added to the extraction medium as phosphatase inhibitors, except where samples were to be processed for in vitro dephosphorylation experiments. For densitometric evaluations, a Sony (Milan, Italy) videocamera and the computer program lmage (version 1.52) from the National lnstitutes of Health (Bethesda, MD) were used.The expression of 0 2 in Escherichia coli was obtained by cloning the 0 2 cDNA (Schmidt et al, 1990) into the pET3c vector (Studier et al, 1990). A synthetic oligonucleotide was used to introduce an Ndel site at the ATG of the 0 2 cDNA clone by polymerase chain reaction.…”
mentioning
confidence: 99%
“…Several other proteins, i.e. maize opaque 2 (Schmidt et al, 1990), CPRF-2 of parsley (Weisshaar et al, 1991), rice RITA-1 , and two bZIP proteins of Antirrhinum (Martinez-Garcia et al, 1998) which do not belong to these families are also known to interact with the Hex-motif (Izawa et al, 1993). All of these DNA-binding proteins share the common structural features of a basic region and a leucine zipper (bZIP) motif which are found in many transcription factors (Landschulz et al, 1988;Vinson et al, 1989).…”
Section: Introductionmentioning
confidence: 99%
“…The amino acid sequence of the Opaque-2 DNA-binding domain is similar to the region of the Em-binding protein (a well characterized ABA-related transcription factor ; Guiltinan, Marcotte & Quatrano, 1990) that binds ABAresponsive promoter elements. The Opaque-2 binding domain also has homology to the DNA-binding domain of a transcription factor that binds to the TGACGT motif (also part of an O2S element) in histone gene promoters (Schmidt et al, 1990 ;Lohmer et al, 1991 ;Lanahan et al, 1992). The O2S motif is found in ABA-regulated genes such as RAB (Mundy, Yamaguchi-Shinozaki & Chua, 1990) and Opaque-2 has been shown to regulate the expression of seed storage proteins during maturation (Lohmer et al, 1991).…”
Section: Transcriptional Regulationmentioning
confidence: 99%