Biochar
amendments add persistent organic carbon to soil and can
stabilize rhizodeposits and existing soil organic carbon (SOC), but
effects of biochar on subsoil carbon stocks have been overlooked.
We quantified changes in soil inorganic carbon (SIC) and SOC to 2
m depth 10 years after biochar application to calcareous soil. The
total soil carbon (i.e., existing SOC, SIC, and biochar-C) increased
by 71, 182, and 210% for B30, B60, and B90, respectively. Biochar
application at 30, 60, and 90 t ha–1 rates significantly
increased SIC by 10, 38, and 68 t ha–1, respectively,
with accumulation mainly occurring in the subsoil (below 1 m). This
huge increase of SIC (mainly CaCO3) is ∼100 times
larger than the inorganic carbon present in the added biochar (0.3,
0.6, or 0.9 t ha–1). The benzene polycarboxylic
acid method showed that the biochar-amended soil contained more black
carbon particles (6.8 times higher than control soil) in the depth
of 1.4–1.6 m, which provided the direct quantitative evidence
for biochar migration into subsoil after a decade. Spectral and energy
spectrum analysis also showed an obvious biochar structure in the
biochar-amended subsoil, accompanied by a Ca/Mg carbonate cluster,
which provided further evidence for downward migration of biochar
after a decade. To explain SIC accumulation in subsoil with biochar
amendment, the interacting mechanisms are proposed: (1) biochar amendment
significantly increases subsoil pH (0.3–0.5 units) 10 years
after biochar application, thus forming a favorable pH environment
in the subsoil to precipitate HCO3
–;
and (2) the transported biochar in subsoil can act as nuclei to precipitate
SIC. Biochar amendment enhanced SIC by up to 80%; thus, the effects
on carbon stocks in subsoil must be understood to inform strategies
for carbon dioxide removal through biochar application. Our study
provided critical knowledge on the impact of biochar application to
topsoil on carbon stocks in subsoil in the long term.