Maize Root Lectins Mediate the Interaction with Herbaspirillum seropedicae via N-Acetyl Glucosamine Residues of Lipopolysaccharides

Balsanelli, Eduardo; Tuleski, Thalita Regina; Baura, Valter A.; Yates, M. G.; Chubatsu, Leda S.; Pedrosa, Fábio de Oliveira; Souza, Emanuel Maltempi de; Monteiro, Rose A.

doi:10.1371/journal.pone.0077001

Cited by 51 publications

(37 citation statements)

References 42 publications

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“…When lipopolysaccharides mediating surface characteristics were studied in more detail, WaaL, the O-antigen ligase, was found to be important for attachment and endophytic colonization, which is probably mediated by plant lectins binding to the bacterial surface (10). In contrast, exopolysaccharide production involved in biofilm formation and attachment to inert surfaces was not important for epiphytic or inner colonization of maize roots by Herbaspirillum (9).…”

Section: Into the Roots: Ways Endophytes Get Inmentioning

confidence: 92%

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Reinhold‐Hurek

Bünger

Burbano

et al. 2015

Annu. Rev. Phytopathol.

618

379

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Land plants interact with microbes primarily at roots. Despite the importance of root microbial communities for health and nutrient uptake, the current understanding of the complex plant-microbe interactions in the rhizosphere is still in its infancy. Roots provide different microhabitats at the soil-root interface: rhizosphere soil, rhizoplane, and endorhizosphere. We discuss technical aspects of their differentiation that are relevant for the functional analysis of their different microbiomes, and we assess PCR (polymerase chain reaction)-based methods to analyze plant-associated bacterial communities. Development of novel primers will allow a less biased and more quantitative view of these global hotspots of microbial activity. Based on comparison of microbiome data for the different root-soil compartments and on knowledge of bacterial functions, a three-step enrichment model for shifts in community structure from bulk soil toward roots is presented. To unravel how plants shape their microbiome, a major research field is likely to be the coupling of reductionist and molecular ecological approaches, particularly for specific plant genotypes and mutants, to clarify causal relationships in complex root communities.

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Section: Into the Roots: Ways Endophytes Get Inmentioning

confidence: 92%

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Reinhold‐Hurek

Bünger

Burbano

et al. 2015

Annu. Rev. Phytopathol.

618

379

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“…Assays of maize colonization by H. seropedicae strains were performed according to Balsanelli et al . (). Briefly, seeds of Zea mays cv.…”

Section: Methodsmentioning

confidence: 99%

Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere

Balsanelli

Tadra-Sfeir

Faoro

et al. 2015

Environmental Microbiology

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Molecular mechanisms of plant recognition and colonization by diazotrophic bacteria are barely understood. Herbaspirillum seropedicae is a Betaproteobacterium capable of colonizing epiphytically and endophytically commercial grasses, to promote plant growth. In this study, we utilized RNA-seq to compare the transcriptional profiles of planktonic and maize root-attached H. seropedicae SmR1 recovered 1 and 3 days after inoculation. The results indicated that nitrogen metabolism was strongly activated in the rhizosphere and polyhydroxybutyrate storage was mobilized in order to assist the survival of H. seropedicae during the early stages of colonization. Epiphytic cells showed altered transcription levels of several genes associated with polysaccharide biosynthesis, peptidoglycan turnover and outer membrane protein biosynthesis, suggesting reorganization of cell wall envelope components. Specific methyl-accepting chemotaxis proteins and two-component systems were differentially expressed between populations over time, suggesting deployment of an extensive bacterial sensory system for adaptation to the plant environment. An insertion mutation inactivating a methyl-accepting chemosensor induced in planktonic bacteria, decreased chemotaxis towards the plant and attachment to roots. In summary, analysis of mutant strains combined with transcript profiling revealed several molecular adaptations that enable H. seropedicae to sense the plant environment, attach to the root surface and survive during the early stages of maize colonization.

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“…An ability to degrade flavonoids would likely confer an important competitive advantage in rhizosphere/root colonization of the host plant by providing both a carbon source and associated detoxification mechanisms. Balsanelli et al [32] showed that surface lipopolysaccharides produced by H. seropedicae strain Smr1 are required for attachment and endophytic colonization of maize plants. They [32] found that the H. seropedicae attachment process is partially mediated by a root lectin that specifically binds N-acetyl glucosamine residues.…”

Section: Introductionmentioning

confidence: 99%

“…Balsanelli et al [32] showed that surface lipopolysaccharides produced by H. seropedicae strain Smr1 are required for attachment and endophytic colonization of maize plants. They [32] found that the H. seropedicae attachment process is partially mediated by a root lectin that specifically binds N-acetyl glucosamine residues.…”

Section: Introductionmentioning

confidence: 99%

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

Lima

Olivares

Oliveira

et al. 2014

Chem. Biol. Technol. Agric.

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Background: Co-inoculation of maize with Herbaspirillum seropedicae and humic substances increases the sizes of plant-associated bacterial populations and enhances grain yields under laboratory and field conditions. Root exudation is a key mechanism in the regulation of plant-bacterial interactions in the rhizosphere; humic matter supplementation is known to change the exudation of H + ions and organic acids from maize roots. Our starting premise was that H. seropedicae and humic acids would modify maize seedling exudation profiles. We postulated that a better understanding of these shifts in exudate profiles might be useful in improving the chemical environment to promote better performance of plant growth-promoting bacteria delivered as bioinoculants. Thus, root exudates of maize were collected and analyzed by gas chromatography-mass spectrometry (GC-MS) and proton nuclear magnetic resonance (

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Maize Root Lectins Mediate the Interaction with Herbaspirillum seropedicae via N-Acetyl Glucosamine Residues of Lipopolysaccharides

Cited by 51 publications

References 42 publications

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere

Root exudate profiling of maize seedlings inoculated with Herbaspirillum seropedicaeand humic acids

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