Maize Transformation

Wang, Kan; Frame, Bronwyn; Ishida, Yuji; Komari, Toshihiko

doi:10.1007/978-0-387-77863-1_31

Cited by 13 publications

(19 citation statements)

References 125 publications

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“…The capacity to produce regenerable calluses from immature embryos appears to be genotypedependent in many species, including maize (El-Itriby et al 2003;Che et al 2006;Wang et al 2009). Conventional transformation procedures in maize involve the use of genotype HI II, which has good characteristics for in vitro culture but poor adaptation to Argentinean agro-ecological conditions.…”

Section: Discussionmentioning

confidence: 98%

“…Green and Philips (1975) first described plant regeneration in maize and obtained embryogenic calluses derived from immature embryos of the A188 inbred line. Additionally, two types of embryogenic calluses have been described for maize: Type I, which are compact and more easily obtained from immature embryos, and Type II, which are friable and maintain the capacity to regenerate plants over a longer period of time (Wang et al 2009). Bohorova et al (1995) opened the discussion about the capacity to produce regenerable callus lines from immature embryos that appear to be genotype-dependent in many species, including maize (Che et al 2006;Wang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines

Gonzalez¹,

Pacheco²,

Oneto³

et al. 2012

Electro Journal of Biotech

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Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC (mature somatic embryos/callus evaluated) x 100], Regeneration Capacity (RC) and Fertile Plant Recovery in greenhouse (FPR, fertile plants/regenerated plants) were recorded. A total of 17 lines reached an EC > 50%, and 14 out of those 17 lines regenerated seedlings. The FPR ranged between 50 and 100%. Also, we selected three promising lines with high agronomic performance, as alternatives to Hi II, in order to be included in a maize transformation scheme via somatic embryogenesis. In addition, we report the usefulness of Single Sequences Repeat (SSRs) in the determination of genetic diversity among 14 divergent lines for somatic embryogenesis response. The seven lines displaying good in vitro behaviour can be crossed to obtain hybrids combining desirable alleles for somatic embryogenesis response and different genetic backgrounds.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines

Gonzalez¹,

Pacheco²,

Oneto³

et al. 2012

Electro Journal of Biotech

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“…Domesticated from teosinte more than 5,000 years ago, maize is the most important food/feed crop and most produced grain among the world top seven cereals (Gewin, 2003;Wang et al, 2009). The world average yield of maize was increased from 19423 hectogram (Hg)/hectare (Ha) in 1961 to 51847 Hg/Ha in 2011 (FAO, 2013), nearly tripled in the past 50 years.…”

Section: Maize (Zea Mays)mentioning

confidence: 99%

“…The kanamycin selection system consists of the nptII gene and the antibiotic kanamycin (or its analogs geneticin G418 or paromomycin) and has been used in early maize transformation assays (Rhodes et al, 1988;Gould et al, 1991;D'Halluin et al, 1992;Lowe et al, 1995). Although growth of single maize cells can be inhibited by kanamycin, large-sized chunks of maize cells are less sensitive to this antibiotic (Wang et al, 2009). The typically used concentration of kanamycin (including G418 and paromomycin) is 50 to 200 mg/L (Rhodes et al, 1988;Gould et al, 1991;D'Halluin et al, 1992;Lowe et al, 1995).…”

Section: Selectable Markersmentioning

confidence: 99%

Genetic transformation of major cereal crops

Ji¹,

Xu²,

Wang³

2013

Int. J. Dev. Biol.

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Of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. Three major cereal crops, rice (Oryza sativa), maize (Zea mays), and wheat (Triticum sp.), provide two-thirds of the world's food energy intake. Although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. Whereas conventional and marker-assisted breeding programs continue to play a major role in crop improvement, genetic engineering has drawn an intense worldwide interest from the scientific community. In the past decade, genetic transformation technologies have revolutionized agricultural practices and millions of hectares of biotech crops have been cultured. Because of its unique ability to insert well-characterized gene sequences into the plant genome, genetic engineering can also provide effective tools to address fundamental biological questions. This technology is expected to continue to be an indispensable approach for both basic and applied research. Here, we overview briefly the development of the genetic transformation in the top seven cereals, namely maize, rice, wheat, barley (Hordeum vulgare), sorghum (Sorghum sp.), oat (Avena sativa), and millets. The advantages and disadvantages of the two major transformation methods, Agrobacterium tumefaciens-mediated and biolistic methods, are also discussed.

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“…Although considered a very efficient method of transforming maize, a possible disadvantage is the occurrence of multiple copies of the gene of interest and complex integration patterns, susceptible to silencing, of gene expression in future generations (Wang and Frame, 2004).…”

Section: Transformation Of Maize Cells Using Microparticle Bombardmentmentioning

confidence: 99%