Maize WRKY114 gene negatively regulates salt-stress tolerance in transgenic rice

Chen, Bo; Chen, Haowei; Luo, Guowei; Li, Wei; Zhang, Xingen; Ma, Qing; Cheng, Beijiu; Cai, Ronghao

doi:10.1007/s00299-019-02481-3

Cited by 62 publications

(21 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because salinity is a common abiotic stress that profoundly affects the growth of plants, how plants respond to salt stress and balance growth and abiotic stress tolerances has long interested scientists (Yang and Guo, 2018b). Many WRKY genes play positive or negative roles in plant salt stress responses (Shang et al, 2010;Liang et al, 2017;Dai et al, 2018;Bo et al, 2020). In this study, we provide evidence that PalWRKY77 is a negative regulator of tolerance to salt stress in Populus alba var.…”

Section: Discussionmentioning

confidence: 78%

The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus

et al. 2021

View full text Add to dashboard Cite

High salinity, one of the most widespread abiotic stresses, inhibits photosynthesis, reduces vegetation growth, blocks respiration and disrupts metabolism in plants. In order to survive their long-term lifecycle, trees, such as Populus species, recruit the abscisic acid (ABA) signaling pathway to adapt to a saline environment. However, the molecular mechanism behind the ABA-mediated salt stress response in woody plants remains elusive. We have isolated a WRKY transcription factor gene, PalWRKY77, from Populus alba var. pyramidalis (poplar), the expression of which is repressed by salt stress. PalWRKY77 decreases salt tolerance in poplar. Furthermore, PalWRKY77 negatively regulated ABA-responsive genes and relieved ABAmediated growth inhibition, indicating that PalWRKY77 is a repressor of the ABA response. In vivo and in vitro assays revealed that PalWRKY77 targets the ABA-and salt-induced PalNAC002 and PalRD26 genes by binding to the W-boxes in their promoters. In addition, overexpression of both PalNAC002 and PalRD26 could elevate salt tolerance in transgenic poplars. These findings reveal a novel negative regulation mechanism for the ABA signaling pathway mediated by PalWRKY77 that results in more sensitivity to salt stress in poplar. This deepens our understanding of the complex responses of woody species to salt stress.

show abstract

Section: Discussionmentioning

confidence: 78%

The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The transcription factor SbWRKY50 from sweet sorghum is negatively involved in the salt response, reducing salt tolerance in A. thaliana by directly binding to the upstream promoter of SOS1 and HKT1 to control ion homeostasis (Song et al, 2020a). The maize ZmWRKY114 gene also negatively regulates salt-stress tolerance in transgenic rice by attenuating ABA signaling (Bo et al, 2020).…”

Section: Induction Of Transcription Factor Expressionmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

View full text Add to dashboard Cite

As two coexisting abiotic stresses, salt stress and alkali stress have severely restricted the development of global agriculture. Clarifying the plant resistance mechanism and determining how to improve plant tolerance to salt stress and alkali stress have been popular research topics. At present, most related studies have focused mainly on salt stress, and salt-alkali mixed stress studies are relatively scarce. However, in nature, high concentrations of salt and high pH often occur simultaneously, and their synergistic effects can be more harmful to plant growth and development than the effects of either stress alone. Therefore, it is of great practical importance for the sustainable development of agriculture to study plant resistance mechanisms under saline-alkali mixed stress, screen new saline-alkali stress tolerance genes, and explore new plant salt-alkali tolerance strategies. Herein, we summarized how plants actively respond to saline-alkali stress through morphological adaptation, physiological adaptation and molecular regulation.

show abstract

“…Most of the TFs that have been implicated in salt-stress tolerance play positive roles, and the overexpression lines represent enhanced salt tolerance with high sensitivity to abscisic acid (ABA), less accumulation of Na + and reactive oxygen species (ROS), or more protective compound content such as proline (Ayub et al, 2020 ; Fernando, 2020 ; Singh et al, 2021 ). A small amount of TFs belongs to WRKY, MYB, or AP2/ERF families, such as PcWRKY33, ZmWRKY114 , and ABA-INSENSITIVE 4 (ABI4) are reported to play negative roles in salt-stress tolerance regulation in a different manner (Cai et al, 2017 ; Bao et al, 2018 ; Bo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

AhABI4s Negatively Regulate Salt-Stress Response in Peanut

et al. 2021

View full text Add to dashboard Cite

Soil salinity is one of the major factors that limit the area of cultivable land and yield potential of crops. The ability of salt tolerance varies with plant species. Peanut (Arachis hypogaea L.) is a moderately salt-sensitive and economically important crop, however, their biological processes involved in salt-stress response remain unclear. In this study, we investigated the role of A. hypogaea L. ABSCISIC ACID INSENSITIVE 4s (AhABI4s) in salt tolerance and elucidated its mode of action in peanuts. The results showed that the downregulation of AhABI4s via whole plant virus-induced gene silencing has enhanced the survival rate, biomass accumulation, and root/shoot ratio of peanut seedlings in response to salt-stress. Transcriptomics, quantitative proteomics, and phosphoproteomic analyses were performed using AhABI4s-silenced and Mock plants. The expression pattern of 15,247 genes, 1,900 proteins, and 2,620 phosphorylation sites were affected by silencing of AhABI4s in peanut leaf and root after sodium chloride (NaCl) treatment. Among them, 63 potential downstream target genes of ABI4 changed consistently at both transcription and translation levels, and the protein/phosphorylation levels of 31 ion transporters/channels were also affected. Electrophoretic mobility shift assays (EMSA) showed that ABI4 was able to bind to the promoters of HSP70, fructokinase (FRK), and pyruvate kinase (PK) coding genes in vitro. In addition, we also detected a binding preference of AhABI4 for CACT(G/T)GCA motif in the promoters of down-regulated genes in peanut leaf. Collectively, the potential downstream targets which were regulated at the levels of transcription and translation, binding preference, and in vivo phosphorylation sites that had been revealed in this study will provide new insight into the AhABI4s-mediated salt tolerance regulation mechanism in peanuts.

show abstract

Maize WRKY114 gene negatively regulates salt-stress tolerance in transgenic rice

Cited by 62 publications

References 67 publications

The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus

The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus

Response Mechanisms of Plants Under Saline-Alkali Stress

AhABI4s Negatively Regulate Salt-Stress Response in Peanut

Contact Info

Product

Resources

About