Major allergen from Amaranthus palmeri pollen is a profilin: Isolation, partial characterisation and IgE recognition

Landa-Pineda, C.M.; Arroyo-Becerra, Analilia; Rosas-Alvarado, Alejandro; Terán, Luis M.; Garcia-Cruz, M.L.; Marchat, Laurence A.; Reyes-López, César A.

doi:10.1016/j.aller.2015.05.002

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…According to frequencies of IgE recognition, profilin is an important allergen in ragweed pollen allergy ( 49 ) and in mugwort pollen allergy ( 18 ). For several other allergen sources, profilins were reported to be major allergens such as for eggplant ( 50 ), Amaranthus ( 51 ), melon ( 52 – 54 ), orange ( 55 ), and oilseed rape ( 56 ).…”

Section: Discussionmentioning

confidence: 99%

The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)

Peterkova,

Trifonova,

Gattinger

et al. 2024

Front. Immunol.

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Pollen from Salsola kali, i.e., saltwort, Russian thistle, is a major allergen source in the coastal regions of southern Europe, in Turkey, Central Asia, and Iran. S. kali-allergic patients mainly suffer from hay-fever (i.e., rhinitis and conjunctivitis), asthma, and allergic skin symptoms. The aim of this study was to investigate the importance of individual S. kali allergen molecules. Sal k 1, Sal k 2, Sal k 3, Sal k 4, Sal k 5, and Sal k 6 were expressed in Escherichia coli as recombinant proteins containing a C-terminal hexahistidine tag and purified by nickel affinity chromatography. The purity of the recombinant allergens was analyzed by SDS-PAGE. Their molecular weight was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and their fold and secondary structure were studied by circular dichroism (CD) spectroscopy. Sera from clinically well-characterized S. kali-allergic patients were used for IgE reactivity and basophil activation experiments. S. kali allergen-specific IgE levels and IgE levels specific for the highly IgE cross-reactive profilin and the calcium-binding allergen from timothy grass pollen, Phl p 12 and Phl p 7, respectively, were measured by ImmunoCAP. The allergenic activity of natural S. kali pollen allergens was studied in basophil activation experiments. Recombinant S. kali allergens were folded when studied by CD analysis. The sum of recombinant allergen-specific IgE levels and allergen-extract-specific IgE levels was highly correlated. Sal k 1 and profilin, reactive with IgE from 64% and 49% of patients, respectively, were the most important allergens, whereas the other S. kali allergens were less frequently recognized. Specific IgE levels were highest for profilin. Of note, 37% of patients who were negative for Sal k 1 showed IgE reactivity to Phl p 12, emphasizing the importance of the ubiquitous cytoskeletal actin-binding protein, profilin, for the diagnosis of IgE sensitization in S. kali-allergic patients. rPhl p 12 and rSal k 4 showed equivalent IgE reactivity, and the clinical importance of profilin was underlined by the fact that profilin-monosensitized patients suffered from symptoms of respiratory allergy to saltwort. Accordingly, profilin should be included in the panel of allergen molecules for diagnosis and in molecular allergy vaccines for the treatment and prevention of S. kali allergy.

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Section: Discussionmentioning

confidence: 99%

The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)

Peterkova,

Trifonova,

Gattinger

et al. 2024

Front. Immunol.

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show abstract

“…284 Since this plant has allergic effect on humans, a study revealed that the allergen in the pollen is profilin (14 kDa protein). 285 Finally, to the best of our knowledge, there are no publications that present novel natural products that were isolated from this plant, though the general chemical composition has been published. 286 No research articles relevant to this review have been published regarding Amaranthus albus and Amaranthus rudis.…”

Section: Amaranthus Palmerimentioning

confidence: 99%

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

Azab¹

2020

ECB

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Identification of allergens and allergen hydrolysates by proteomics and metabolomics: A comparative study of natural and enzymolytic bee pollen

Tao

Yin

et al. 2022

Food Research International

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Major allergen from Amaranthus palmeri pollen is a profilin: Isolation, partial characterisation and IgE recognition

Cited by 7 publications

References 37 publications

The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)

The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

Identification of allergens and allergen hydrolysates by proteomics and metabolomics: A comparative study of natural and enzymolytic bee pollen

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