Major components of orange oil inhibit Staphylococcus aureus growth and biofilm formation, and alter its virulence factors

Federman, Cassandra; Christopher, D.; Biswas, Debabrata

doi:10.1099/jmm.0.000286

Cited by 28 publications

(17 citation statements)

References 41 publications

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“…In the current study, we found significant increases in specific constitutes of the essential oil of plants subjected to salinity and SA such as linalool, camphor, borneol, and caryophyllene oxide. For example, Federman et al ( 2016 ) reported that the major oil constitute of the orange was the linalool responsible for inhibiting growth and biofilm formation in S. aureus .…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid-Regulated Antioxidant Mechanisms and Gene Expression Enhance Rosemary Performance under Saline Conditions

et al. 2017

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Salinity stress as a major agricultural limiting factor may influence the chemical composition and bioactivity of Rosmarinus officinallis L. essential oils and leaf extracts. The application of salicylic acid (SA) hormone may alleviate salinity stress by modifying the chemical composition, gene expression and bioactivity of plant secondary metabolites. In this study, SA was applied to enhance salinity tolerance in R. officinallis. R. officinallis plants were subjected to saline water every 2 days (640, 2,000, and 4,000 ppm NaCl) and 4 biweekly sprays of SA at 0, 100, 200, and 300 ppm for 8 weeks. Simulated salinity reduced all vegetative growth parameters such as plant height, plant branches and fresh and dry weights. However, SA treatments significantly enhanced these plant growth and morphological traits under salinity stress. Salinity affected specific major essential oils components causing reductions in α-pinene, β-pinene, and cineole along with sharp increases in linalool, camphor, borneol, and verbenone. SA applications at 100–300 ppm largely reversed the effects of salinity. Interestingly, SA treatments mitigated salinity stress effects by increasing the total phenolic, chlorophyll, carbohydrates, and proline contents of leaves along with decline in sodium and chloride. Importantly, this study also proved that SA may stimulate the antioxidant enzymatic mechanism pathway including catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) as well as increasing the non-enzymatic antioxidants such as free and total ascorbate in plants subjected to salinity. Quantitative real-time PCR analysis revealed that APX and 3 SOD genes showed higher levels in SA-treated rosemary under salinity stress, when compared to non-sprayed plants. Moreover, the expression level of selected genes conferring tolerance to salinity (bZIP62, DREB2, ERF3, and OLPb) were enhanced in SA-treated rosemary under salt stress, indicating that SA treatment resulted in the modulation of such genes expression which in turn enhanced rosemary tolerance to salinity stress.

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Section: Discussionmentioning

confidence: 99%

Salicylic Acid-Regulated Antioxidant Mechanisms and Gene Expression Enhance Rosemary Performance under Saline Conditions

et al. 2017

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“…aureus Inhibits Staph. aureus internalization by inhibiting NF-κB activation Citral and linalool [101] MAC-T Staph. aureus Inhibits Staph.…”

Section: Plant-derived Compoundsmentioning

confidence: 99%

“…It was found out that major components of CDO, citral, and linalool are responsible for inhibition, owing to their abilities to alter the expression of Staph. aureus virulence genes [101]. On the contrary, CDO has also been reported to not impair the function of polymorphonuclear leukocytes, which play an important role in immune response against mastitis, at the same time inhibiting bacterial growth [102].…”

Section: Plant-derived Compoundsmentioning

confidence: 99%

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Cheng

Han

2020

Asian-Australas J Anim Sci

302

205

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Bovine mastitis, an inflammation of the mammary gland, is the most common disease of dairy cattle causing economic losses due to reduced yield and poor quality of milk. The etiological agents include a variety of gram-positive and gram-negative bacteria, and can be either contagious (e.g., Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma spp.) or environmental (e.g., Escherichia coli, Enterococcus spp., coagulase-negative Staphylococcus, Streptococcus uberis). Improving sanitation such as enhanced milking hygiene, implementation of post-milking teat disinfection, maintenance of milking machines are general measures to prevent new cases of mastitis, but treatment of active mastitis infection is dependant mainly on antibiotics. However, the extensive use of antibiotics increased concerns about emergence of antibiotic-resistant pathogens and that led the dairy industries to reduce the use of antibiotics. Therefore, alternative therapies for prevention and treatment of bovine mastitis, particularly natural products from plants and animals, have been sought. This review provides an overview of bovine mastitis in the aspects of risk factors, control and treatments, and emerging therapeutic alternatives in the control of bovine mastitis.

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“…Various studies have shown the antimicrobial activity of components of essential oils, such as citral, on strains of S. aureus , inhibiting biofilm formation or altering virulence factors. It is known that essential oils and major components can act to deregulate virulence genes, in addition to antibacterial, antifungal, analgesic, antispasmodic, and antiparasitic activities, in addition to fighting nerve disorders 15 , 17 , 18 . Despite the antibacterial properties of this oil against gram-positive and gram-negative bacteria, its prolonged use has not shown resistance 19 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus

Oliveira

Selis

Sampaio

et al. 2021

Sci Rep

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Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.

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Major components of orange oil inhibit Staphylococcus aureus growth and biofilm formation, and alter its virulence factors

Cited by 28 publications

References 41 publications

Salicylic Acid-Regulated Antioxidant Mechanisms and Gene Expression Enhance Rosemary Performance under Saline Conditions

Salicylic Acid-Regulated Antioxidant Mechanisms and Gene Expression Enhance Rosemary Performance under Saline Conditions

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus

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