Major Constituents and Cytotoxic Effects of Ajuga chamaecistus ssp. tomentella

Sadati, Nargess; Jenett‐Siems, Kristina; Siems, Karsten; Ardekani, Mohammad Reza Shams; Hadjiakhoondi, Abbas; Akbarzadeh, Tahmineh; Ostad, Seyed Nasser; Khanavi, Mahnaz

doi:10.5560/znc.2012.67c0275

Cited by 6 publications

(5 citation statements)

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“…4). This result is in agreement with the work of Sadati et al (2012) who documented the cytotoxic effect of Ajuga chamaecistus spp. tomentella on the T47D, Caco-2, and HT-29 cancer cell lines.…”

Section: Resultssupporting

confidence: 93%

Evaluation of the antiproliferative effect of selected plant extracts on colon and skin cancer cell lines

Oran¹,

Yousef²,

Jaffal³

2022

J App Pharm Sc

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The aim of this study was to assess the antiproliferative effect of extracts obtained from medicinal plants that grow wild in Jordan against the human melanoma (WM136-1A) and colon cancer (Caco-2 cancer) cell lines using fibroblast cell lines as control cells. The selected plants are Varthemia iphionoides (Asteraceae), Micromeria myrtifolia (Lamiaceae), Micromeria nervosa (Lamiaceae), Origanum dayi (Lamiaceae), Ajuga chia (Lamiaceae), Salvia palaestina (Lamiaceae), and Bongardia chrysogonum (Berberidaceae). The results showed that M. nervosa and O. dayi exhibited antiproliferative activity against Caco-2 cancer cell line, with half-maximal inhibitory concentrations (IC 50 ) that equal 98 ± 2.5 and 73.6 ± 3.4 μg/ml, respectively. Furthermore, A. chia, B. chrysogonum, and O. dayi exhibited antiproliferative activity against the WM136-1A cancer cell line, with IC 50 that equals 400 ± 5.1, 98.1 ± 3.2, and 245 ± 3.6 μg/ml, respectively. None of the extracts had an effect on fibroblast cells at any used concentration. In conclusion, the extracts of M. nervosa, O. dayi, A. chia, and B. chrysogonum showed promising antiproliferative potential and can be good candidates for the development of novel anticancerous agents.

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Section: Resultssupporting

confidence: 93%

Evaluation of the antiproliferative effect of selected plant extracts on colon and skin cancer cell lines

Oran¹,

Yousef²,

Jaffal³

2022

J App Pharm Sc

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“…extracts on B16.F10 murine melanoma and C26 colon carcinoma cells proliferation, 5 × 10 3 cancer cells/well were cultured in 96-well plates for 24 h. The range of concentrations for each extract was selected based on previous studies regarding in vitro cytotoxic activity of Ajuga sp. (Sadati et al, 2012) and the effect was measured in triplicate samples for the controls (cells incubated in medium alone) and for each concentration of the vegetal extracts. To screen for ethanol toxicity, cells were incubated with the same concentrations of the solvent as those used for the preparation of the ethanolic extracts.…”

Section: Methodsmentioning

confidence: 99%

Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development

et al. 2019

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Backround: Ajuga species have been used in traditional medicine for their diuretic, anti-inflammatory, wound-healing, and hepatoprotective properties. Purpose: The phytochemical profile and anticancer potential of three Ajuga sp. ( A. genevensis, A. chamaepitys , and A. laxmannii ) from Romania was investigated. Materials and Methods: The phytochemicals were extracted from the aerial parts of Ajuga sp. by using different solvents and methods. The hydroalcoholic extracts were examined for total phenolic, flavonoid and iridoid contents, and HPLC/MS was used to analyze the polyphenolic compounds and iridoids. The phytochemical profile was also evaluated by principal component analysis in connection with antitumor efficacy of extracts. The antiproliferative potential was evaluated using the ELISA BrdU-colorimetric immunoassay. Western Blot with regard to inflammatory protein NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) p65 subunit expression in cell lysates was performed. Quantification of oxidative stress marker malondialdehyde (MDA) was determined by high-performance liquid chromatography (HPLC). Enzymatic and non-enzymatic antioxidant capability was assessed by measuring catalase activity and by evaluating the total antioxidant capacity (TAC) of treated cells. Results: Ajuga laxmannii ethanol extract showed the highest total phenolic and flavonoid content, while A. genevensis ethanol extract was more abundant in iridoids. The overall cytostatic effect of the investigated plant extracts was exerted through strong inhibitory actions on NF-κB, the key molecule involved in the inflammatory response and via oxidative stress modulatory effects in both murine colon carcinoma and melanoma cell lines. Conclusion: Ajuga laxmannii showed the most significant antitumor activity and represents an important source of bioactive compounds, possibly an additional form of treatment alongside conventional anticancer drugs.

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“…Tomentella showed significant cytotoxic activity against colon carcinoma (HT-29), colorectal adenocarcinoma cells (Caco-2), breast ductal carcinoma (T47D), and Swiss mouse embryo fibroblasts (NIH-3T3), while the diethyl ether fraction was moderately cytotoxic against HT-29. 15 Furthermore, the antiproliferative activity of Ajugalide-B (ATMA) from A. taiwanensis was tested against hepatoma (HepG2), lung adenocarcinoma (A549), gastric carcinoma (AGS), and colon carcinoma (HT29). ATMA disrupted the focal adhesion complex and reduced phosphorylation by paxillin and focal adhesion kinase (FAK).…”

Section: Resultsmentioning

confidence: 99%

In vitro Assessment of Cytotoxic Potential of Ajuga orientalis L. Methanolic Extract on MCF-7and MRC-5 cells

2022

TJNPR

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Medicinal plants have played an essential part in the discovery and development of medications during the past few decades. Many medicinal plants provide unique treatments with high efficacy and few side effects compared to the known standard pharmaceuticals used to treat the most debilitating diseases, such as cancer. 1 Medicinal plants make up roughly 20% of Jordan's flora. 2,3 As a result, many are used in traditional medicine and pharmaceutical production. 4,5 The Lamiaceae family includes the genus Ajuga as one of its members. Ajuga s a genus of 50 species of evergreen, clump-forming rhizomatous, annual, or perennial herbs that grow to a height of 5-50 cm and are found primarily in Asia, Africa, Australia, and North America, in addition to Europe. 6 Ajuga orientalis L. (also known as Eastern bugle) is a 20-40cm tall annual herbaceous flowering plant with opposite leaves that grows in humid areas (Ajloun, Salt, Amman, and Karak). 2 A. orientalis is a medicinal plant with diverse biological activities. It is used in traditional medicine worldwide to treat rheumatism, gout, diabetes, asthma, malaria, and gastrointestinal disorders. 7,8 A. orientalis has antibacterial, antitumor, antioxidant, and anti-inflammatory properties. [6][7][8][9][10][11] This study aimed to assess the cytotoxic activity of methanolic extract of the aerial part of Ajuga orientalis L. on the human breast cancer cell line (MCF-7).

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Major Constituents and Cytotoxic Effects of Ajuga chamaecistus ssp. tomentella

Cited by 6 publications

References 0 publications

Evaluation of the antiproliferative effect of selected plant extracts on colon and skin cancer cell lines

Evaluation of the antiproliferative effect of selected plant extracts on colon and skin cancer cell lines

Biologically Active Ajuga Species Extracts Modulate Supportive Processes for Cancer Cell Development

In vitro Assessment of Cytotoxic Potential of Ajuga orientalis L. Methanolic Extract on MCF-7and MRC-5 cells

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