Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes

Wang, Lin‐Fa; Kattenbelt, Jacki A.; Gould, Allan R.; Pritchard, L.I.; Crameri, Gary; Eaton, Bryan T.

doi:10.1099/0022-1317-75-9-2421

Cited by 14 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite high neutralizing Abs detection following BTV15 single serotype infection, ELISA detecting VP7 Abs showed a mean PN at 35 dpi just above the positivity threshold. This is consistent with previous reports showing that significant immunological differences exist between BTV15 and other BTV serotypes and that monoclonal antibodies raised against BTV1 VP7 failed to react with BTV15 VP7 [ 37 ]. When assessing diagnostic tools aiming at non-serotype specific detection, it would be therefore advisable to include distantly related strains in the test panel to cover most of the genetic variability displayed by BTV proteins.…”

Section: Discussionsupporting

confidence: 93%

Experimental bluetongue virus superinfection in calves previously immunized with bluetongue virus serotype 8

Martinelle

Pozzo

Sarradin

et al. 2016

Vet Res

View full text Add to dashboard Cite

The effect of a superinfection with bluetongue virus serotype 1 (BTV1) was evaluated on two groups of four calves. One group received a commercial inactivated BTV serotype 8 (BTV8) vaccine. This group and the non-vaccinated group of calves were challenged twice (4 months apart) with the European BTV8 strain isolated during the 2006–2007 epidemics. Calves were then infected with a BTV1 inoculum which was found to be unexpectedly contaminated by BTV serotype 15 (BTV15). BTV1 and BTV15 single infections were performed on two other groups of three BTV naïve calves. A severe clinical picture was obtained after superinfection with BTV1/BTV15 in both vaccinated and non-vaccinated animals and after challenge with BTV8 in non-vaccinated animals. BTV1 and BTV15 single infection caused only very slight clinical signs. After superinfection and at the viraemic peak, there were an average of above 1000 times more BTV15 genomic copies than BTV1 ones. BTV1 RNA could be detected only in the spleen of one calf whereas BTV15 RNA was found in 15 organs of seven different animals. BTV8 immunization whether it was acquired through vaccination and challenges or challenges alone did not change BTV1 or BTV15 RNA detection in superinfected animals. However in these animals a partial cross neutralization between BTV8 and BTV1 might be involved in the lower BTV1 replication versus BTV15. Infection with different serotypes can occur also in the field. Interference between virus strains, genetic reassortment and cross-protection were considered as mechanisms to explain the clinical outcomes and the other virological and immunological findings in the course of BTV1/BTV15 superinfection.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0357-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 93%

Experimental bluetongue virus superinfection in calves previously immunized with bluetongue virus serotype 8

Martinelle

Pozzo

Sarradin

et al. 2016

Vet Res

View full text Add to dashboard Cite

show abstract

“…ND Place of isolation not determined. GenBank accession numbers of the BTV sequences: BTV‐10 ND (Yu and others 1988): X06463; BTV‐17 (Kowalik and others 1990a): M11787; BTV‐11 ND (Kowalik and others 1990b): M32102; BTV‐2 USA (Kowalik and Li 1991): M64997; BTV‐12 China, (Bonneau and others 2000)3: AF172829; BTV‐8 USA (Wilson and others 2000): AF188671; BTV‐1 South Africa (Wade‐Evans 1990): M17438; BTV‐16 China (Bonneau and others 2000)3: AF172831; BTV‐3 China (Bonneau and others 2000)3: AF172827; BTV‐1 China (Bonneau and others 2000)3: AF172825; BTV‐2 China (Bonneau and others 2000)3: AF1728261; BTV‐4 China (Bonneau and others 2000)3: AF172828; BTV‐1 Australia (Eaton and others 2000)3: M63417; BTV‐23 India (Kataria and others 2000)3: AJ277802; BTV‐2 USA 2 (Wilson and others 2000): AF188672; BTV‐2 USA 3 (Wilson and others 2000): AF188674; BTV‐2 Corsica (Sailleau and Zientara 2000)3: AF346302; BTV‐15 China (Bonneau and others 2000)3: AF172830; BTV‐15 Australia (Wang and others 1994): L11724 3 Not referenced to published papers…”

Section: Resultsmentioning

confidence: 99%

Identification of bluetongue virus serotype 2 (Corsican strain) by reverse‐transcriptase PCR reaction analysis of segment 2 of the genome

et al. 2002

View full text Add to dashboard Cite

In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.

show abstract

“…5). Amino acid sequence from VP7, a structural protein, which along with VP3 forms the viral capsid [27], is the protein primarily used for phylogenetic analysis of Orbiviruses and exhibits the highest level of conservation within members of the Orbivirus genus with intraspecies amino acid identities between 83-99% [28,29,30]. VRDL3 VP7 exhibited only 19% amino acid identity to the closest Orbiviruses, and phylogenetic analysis revealed a deep, weakly bootstrap-supported pairing to the Great Island Broadhaven virus (Fig.…”

Section: Reovirusesmentioning

confidence: 99%

“…Differentiation of species is largely based upon vector transmission, overall amino acid identity and phylogenetic analysis of viral core (segment 7, VP7) or the RNA-dependent RNA polymerase (segment 2) [29,44,45]. Within the Orbiviruses, amino acid identities between serotypes, such as BTV-1 through BTV-4 are typically above 80% [28,30,46] or 94% in EPHDV [29] within segments 2 and 7. Divergence between BTV and the less well characterized Yunnan, SRCV or BRDV exhibits between 21-37% identity within segment 2 and 7 [44,45,47].…”

Section: Identification Of Novel Virusesmentioning

confidence: 99%

Rapid Identification of Known and New RNA Viruses from Animal Tissues

et al. 2008

View full text Add to dashboard Cite

Viral surveillance programs or diagnostic labs occasionally obtain infectious samples that fail to be typed by available cell culture, serological, or nucleic acid tests. Five such samples, originating from insect pools, skunk brain, human feces and sewer effluent, collected between 1955 and 1980, resulted in pathology when inoculated into suckling mice. In this study, sequence-independent amplification of partially purified viral nucleic acids and small scale shotgun sequencing was used on mouse brain and muscle tissues. A single viral agent was identified in each sample. For each virus, between 16% to 57% of the viral genome was acquired by sequencing only 42–108 plasmid inserts. Viruses derived from human feces or sewer effluent belonged to the Picornaviridae family and showed between 80% to 91% amino acid identities to known picornaviruses. The complete polyprotein sequence of one virus showed strong similarity to a simian picornavirus sequence in the provisional Sapelovirus genus. Insects and skunk derived viral sequences exhibited amino acid identities ranging from 25% to 98% to the segmented genomes of viruses within the Reoviridae family. Two isolates were highly divergent: one is potentially a new species within the orthoreovirus genus, and the other is a new species within the orbivirus genus. We demonstrate that a simple, inexpensive, and rapid metagenomics approach is effective for identifying known and highly divergent new viruses in homogenized tissues of acutely infected mice.

show abstract

Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes

Cited by 14 publications

References 26 publications

Experimental bluetongue virus superinfection in calves previously immunized with bluetongue virus serotype 8

Experimental bluetongue virus superinfection in calves previously immunized with bluetongue virus serotype 8

Identification of bluetongue virus serotype 2 (Corsican strain) by reverse‐transcriptase PCR reaction analysis of segment 2 of the genome

Rapid Identification of Known and New RNA Viruses from Animal Tissues

Contact Info

Product

Resources

About