Major histocompatibility complex class II polymorphic variants are associated with asthma predisposition in the Punjabi population of Lahore, Pakistan

Abstract

Introduction Various genome wide association studies have manifested that Major Histocompatibility Complex (MHC) region on chromosome 6p21 houses many potential candidate genes for asthma. Objective This Case‐Control association study was planned to determine the association of 10 Single Nucleotide Polymorphisms (SNPs), residing within and around MHC genes’ region on chromosome 6p21, with Asthma in Punjabi population of Lahore, Pakistan. Methods A total of 161 subjects, 61 physician‐diagnosed asthma patients and 100 age‐matched healthy controls, were recruited from Lahore, a city in Punjab. Ten single nucleotide polymorphisms (SNPs) (rs9378249, rs2070600, rs404860, rs6689, rs1049124, rs1063355, rs1049225, rs1049219, rs7773955 and rs928976) located within or near AGER, NOTCH and HLA genes in MHC region, were genotyped in both patients and controls using single base extension reaction and capillary electrophoresis‐based genetic analyser. Statistical models were applied using SHEsis Plus. Results were adjusted for various cofactors (age, gender and environment) and by applying multiple corrections. Haplotype and linkage disequilibrium analyses were performed on Haploview software v4.1. Results Three of the studied SNPs rs1049124, rs1049219 and rs7773955 show independent significant association with asthma under allelic and genotypic models. Two of the haplotypes, H7 and H13, “CTAATTT” and “CCACTAT”, respectively, for rs2070600, rs404860, rs6689, rs1049124, rs1063355, rs1049219 and rs7773955, are found to be significantly associated with the disease. Conclusion This study reports association of SNP variants residing on HLA‐DQB1 and HLA‐DQA2 genes and haplotypes H7 and H13 on genomic region 6p21 with Asthma in the Punjabi population of Lahore, Pakistan.

Effect of asthma on the English-speaking and reading comprehension ability of dusty allergy affected school children

“…This was done largely to prevent enrolling children who had asthma rather than chronic obstructive pulmonary disease. 2° FEV1 was shown to be preferred to PEFR in a recent study (Aslam et al, 2021) while evaluating patients who were presenting to the emergency department (ED) with an acute exacerbation of chronic obstructive pulmonary disease.…”

“…Alternately, a healthcare professional may just use a watch to gauge the appropriate moment to talk. Another study examining a larger number of child patients and evaluating prehospital care providers using the speaking time method during evaluation of patients with an acute exacerbation of asthma could be designed in order to further validate the utility of speaking time as an accurate, objective estimate of PEFR (Aslam et al, 2021). Using a peak flow meter, as in our investigation, the PEFR would be calculated after the speaking time was obtained.…”

Effect of food processing on the immunoreactivity of gliadin

“…From the above, there is a correlation between the HLA‐DQA1 and HLA‐DQB1 genetic markers and clinical features of CD (Fernández‐Mestre et al ., 2023). Each MHC class II gene has many possible variations, allowing the immune system to react to a wide range of foreign invaders (Arentz‐Hansen et al ., 2004; Aslam et al ., 2021). Hundreds of different versions of the HLA‐DQA1 gene, called alleles, have been identified, each of which is given a particular number, such as HLA‐DQA1*05:01 (Vianna et al ., 2020).…”

Frequency of Vitamin D Deficiency in Patients of Asthma

“…Asthma is one of the most prevalent pulmonary pathologies around the world [ 1 ]. Its incidence is on the rise in Pakistan [ 2 ]; this could be due to the improved practices of screening and diagnosis or simply a result of overt environmental pollution [ 3 ]. Over the last quarter of a century, advancements have been made in treatment and intervention that have improved the quality of life for asthmatics substantially [ 4 ].…”