Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

Bohórquez, Michel David; Ordóñez, Diego; Suárez, Carlos F.; Vicente, Belén; Vieira, Carmen Baur; López‐Abán, Julio; Muro, A. Di; Ordóñez, Iván; Patarroyo, Manuel A.

doi:10.3389/fgene.2019.01293

Cited by 22 publications

(27 citation statements)

References 83 publications

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“…Gir MDMs showed higher expression of Bovine Leukocyte Antigen (BOLA) family (BOLA-DQ A2, logFC = -6.9 and P = 3.80E-10; BOLA-DQA5, logFC = -6.2 and P = 7.68E-08; BOLA-DQB, logFC = -3.5 and P = 0.0006; Fig 3A; S4 Table) and C-C chemokine receptor type 5 (CCR5; logFC = -1.9 and P = 0.0006; Fig 3A; S4 Table). Interestingly, BoLA receptor family mediate antigen presentation to T-lymphocytes and its variant alleles has been linked to differences in resistance to many disease, including mastitis caused by gram negative bacteria [42][43][44]. The CCR5 receptor acts on leucocyte recruitment to inflammation site [45] and, coupled with BOLA, might influence differential outcomes of immune response in each bovine breed.…”

Section: Breed-specific Bovine Mdm Transcriptional Signatures and Itsmentioning

confidence: 99%

Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections

Daibert¹,

Biagi

Vieira³

et al. 2020

PLoS ONE

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Macrophages are classified upon activation as classical activated M1 and M2 anti-inflammatory regulatory populations. This macrophage polarization is well characterized in humans and mice, but M1/M2 profile in cattle has been far less explored. Bos primigenius taurus (taurine) and Bos primigenius indicus (indicine) cattle display contrasting levels of resistance to infection and parasitic diseases such as C57BL/6J and Balb/c murine experimental models of parasite infection outcomes based on genetic background. Thus, we investigated the differential gene expression profile of unstimulated and LPS stimulated monocyte-derived macrophages (MDMs) from Holstein (taurine) and Gir (indicine) breeds using RNA sequencing methodology. For unstimulated MDMs, the contrast between Holstein and Gir breeds identified 163 Differentially Expressed Genes (DEGs) highlighting the higher expression of C-C chemokine receptor type five (CCR5) and BOLA-DQ genes in Gir animals. LPS-stimulated MDMs from Gir and Holstein animals displayed 1,257 DEGs enriched for cell adhesion and inflammatory responses. Gir MDMs cells displayed a higher expression of M1 related genes like Nitric Oxide Synthase 2 (NOS2), Toll like receptor 4 (TLR4), Nuclear factor NF-kappa-B 2 (NFKB2) in addition to higher levels of transcripts for proinflammatory cytokines, chemokines, complement factors and the acute phase protein Serum Amyloid A (SAA). We also showed that gene expression of inflammatory M1 population markers, complement and SAA genes was higher in Gir in buffy coat peripheral cells in addition to nitric oxide concentration in MDMs supernatant and animal serum. Co-expression analyses revealed that Holstein and Gir animals showed different transcriptional signatures in the MDMs response to LPS that impact on cell cycle regulation, leukocyte migration and extracellular matrix organization biological processes. Overall, the results suggest that Gir animals show a natural propensity to generate a more pronounced M1 inflammatory response than Holstein, which might account for a faster immune response favouring resistance to many infection diseases.

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Section: Breed-specific Bovine Mdm Transcriptional Signatures and Itsmentioning

confidence: 99%

Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections

Daibert¹,

Biagi

Vieira³

et al. 2020

PLoS ONE

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“…Currently, polymerase chain reaction-sequence based typing (PCR-SBT) [16][17][18][19][20][21][22][23][24][25][26][27] and target next generation sequencing (Target-NGS) [28] are the most commonly employed and powerful tools used in this type of analysis. However, these techniques have been applied in studies which have included only a handful of the over 800 recognized cattle breeds worldwide [16][17][18][19][20][21][22][23][24][25][26][27][28]. Consequently, there are still a number of breeds that remain uncharacterized, and this number increases when local native bovine breeds are considered [17,18,26,29].…”

Section: Introductionmentioning

confidence: 99%

“…As a result of these studies there are 144 described BoLA-DRB3 alleles for bovines, and 303 subtypes listed in the Immuno Polymorphism Database (IPD-MHC) [30,31]. In addition, previous studies [16][17][18][19][20][21][22][23][24][25][26][27] have shown an even distribution of BoLA-DRB3 polymorphisms between the major bovine types (Bos taurus and B. indicus) and breeds. This is likely as a result of various factors including breed origin, artificial or natural selection and geographical distribution.…”

Section: Introductionmentioning

confidence: 99%

Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar

Giovambattista

Moe

Polat³

et al. 2020

BMC Genet

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Background: Myanmar cattle populations predominantly consist of native cattle breeds (Pyer Sein and Shwe), characterized by their geographical location and coat color, and the Holstein-Friesian crossbreed, which is highly adapted to the harsh tropical climates of this region. Here, we analyzed the diversity and genetic structure of the BoLA-DRB3 gene, a genetic locus that has been linked to the immune response, in Myanmar cattle populations. Methods: Blood samples (n = 294) were taken from two native breeds (Pyer Sein, n = 163 and Shwe Ni, n = 69) and a cattle crossbreed (Holstein-Friesian, n = 62) distributed across six regions

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“…Despite its central role in the immune response, the genetic diversity of the BoLA-DRB3 gene has been characterized in a limited number of breeds and cross-breeds from Europe, Asia and the Americas using polymerase chain reaction-sequence based typing (PCR-SBT; [16][17][18][19][20][21][22][23][24][25][26][27][28][29]) and target next generation sequencing (Target-NGS; [30]), the most powerful tools used to identify diversity of BoLA-DRB3 alleles in cattle breeds. Until now, private African BoLA-DRB3 alleles have been reported by authors using indirect techniques, such as PCR-RFLP, followed by cloning and sequencing [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

BoLA-DRB3 gene haplotypes show divergence in native Sudanese cattle from Taurine and Zebu breeds

Salim

Takeshima

Moustafa

et al. 2020

Preprint

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Autochthonous Sudanese cattle breeds, namely Baggara for beef and Butana and Kenana for dairy, are characterized by their adaptive characteristics and high performance in hot and dry agro-ecosystems, are used largely by nomadic and semi-nomadic pastoralists. Here we analyzed the diversity and genetic structure of the BoLA-DRB3 gene, a genetic locus linked to the immune response, for the indigenous cattle of Sudan and in the context of the global cattle repository. Blood samples (n=225) were taken from three indigenous breeds (Baggara; n=113, Butana; n= 60 and Kenana; n=52) distributed across six regions of Sudan. Nucleotide sequences were genotyped using the sequence-based typing method. Sequence electropherograms were analyzed using the Assign SBT software. We describe 53 alleles, including seven new, novel alleles. In the Baggara breed the number of alleles was 46 (40 previously reported and six new ones), 33 in the Kenana breed (28 previously reported and five new ones), and 33 in the Butana breed (28 previously reported and five new ones). Venn analysis of Sudanese breeds with Southeast Asian, European and American cattle showed 115 alleles of which 14 were unique to Sudanese breeds. Three of the alleles exhibited gene frequency of > 0.5%, representing 26% of the 53 alleles detected in the native Sudanese cattle. Observed versus expected heterozygosity was higher than 0.93 in all three breeds analyzed and equilibrium status revealed by Hardy-Weinberg Equilibrium suggests pure genetic drift. Gene frequency distributions of Baggara cattle showed an even distribution ( P = 0.016), consistent with the theoretical proportion expected under balancing selection pressure as opposed to positive or neutral selection. In contrast, Butana and Kenana cattle ( P = 0.225 and P = 0.138, respectively) were more congruent with neutral selection, similar to the results obtained for most of the cattle breeds analyzed so far. Sudanese cattle breeds were located within a narrow cloud in an intermediate position between the Zebu and Taurine breeds and close to other Southeast Asian breeds, in accordance with the composite origin of these native breeds, which is also reinforced by the presence of African and Zebu unique BoLA-DRB3 alleles within these breeds. The results of the Principal Component Analysis were in agreement with the overall clustering pattern observed on the NJ and/or UPGMA trees. These results contribute to our understanding of the genetic diversity and distribution pattern of BoLA-DRB3 gene alleles in Sudanese cattle breeds and provide insight into their uniqueness in their ability to survive arrays of tropical diseases and reproduce well in Sudan's harsh environment.

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Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

Cited by 22 publications

References 83 publications

Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections

Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections

Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar

BoLA-DRB3 gene haplotypes show divergence in native Sudanese cattle from Taurine and Zebu breeds

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