Major histocompatibility complex class IIA and IIB genes of Nile tilapia Oreochromis niloticus: Genomic structure, molecular polymorphism and expression patterns

Pang, Ji-cai; Gao, Fengying; Lu, Maixin; Xing, Yongna; Zhu, Hai‐Lei; Ke, Xiaoli

doi:10.1016/j.fsi.2012.11.048

Cited by 119 publications

(59 citation statements)

References 36 publications

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“…MHC class II α sequences have been cloned and identified in several teleosts, including gilthead seabream (Sparus aurata) (Cuesta, Esteban, & Meseguer, 2006), large yellow croaker (Larimichthys crocea) (Yu, Ao, & Chen, 2010), Miiuy croaker (Miichthys miiuy) (Xu, Sun, Shi, Cheng, & Wang, 2011), Nile tilapia (Oreochromis niloticus) (Pang et al, 2013), and blunt snout bream (Megalobrama amblycephala) (Luo et al, 2014). Pathogen infection or immune stimulation can dramatically increase mRNA expression of MHC class II α (Cuesta, Esteban, & Meseguer, 2006;Yu, Ao, & Chen, 2010;Luo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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The MHC (major histocompatibility complex) class II family plays a major role in vertebrate immune systems and is normally found only on antigen-presenting cells. In this study, we identified the gene encoding the α (PaMHCIIα) chain of MHC class II molecules in ayu (Plecoglossus altivelis). Phylogenetic analysis showed that PaMHCIIα was tightly grouped with MHC class IIα in rainbow smelt (Osmerus mordax) and salmon. PaMHCIIα transcripts were detected in all tested tissues, with high levels in the spleen, head kidney, and gill; expression increased significantly upon Vibrio anguillarum infection. PaMHCIIα mRNA expression was also up-regulated in monocytes/macrophages (MO/MФ) after in vitro stimulation with V. anguillarum. We prepared antibodies for detecting N-glycosylation of PaMHCIIα or neutralizing PaMHCIIα on ayu MO/MФ. Western blot analysis showed that native PaMHCIIα was indeed N-glycosylated. Moreover, PaMHCIIα neutralization not only significantly inhibited ayu MO/MФ phagocytosis, but also reduced the ability of V. anguillarum to induce MO/MФ-cytokine expression. Overall, these findings reveal that the important role of PaMHCIIα against bacterial challenge, and it likely contributes to pathogen responsiveness in ayu MO/MФ.

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Section: Introductionmentioning

confidence: 99%

Untitled

Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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“…Specific alleles of MHC-II have been shown to correlate to disease resistance in tilapia (Pang et al, 2013), goldfish (Alimuddin et al, 2011;Hayuningtyas et al, 2013), eel (Li et al, 2014), and channel catfish (Moulana et al, 2008). This study aimed to find the candidate MHC-II marker on molecular marker-based selection to produce catfish resistant to MAS.…”

Section: Mhc-ii Marker Potential Linked To Motile Aeromonad Septicaemmentioning

confidence: 99%

“…Pang et al (2013) reported that MHC genes showed an important role in immune defense and disease control. High polymorphism is one of the most important characters of MHC genes, which provide the basis for screening disease resistant genes associated with molecular markers.…”

Section: (Rommy Suprapto)mentioning

confidence: 99%

MHC-II MARKER POTENTIAL LINKED TO MOTILE AEROMONAD SEPTICAEMIA DISEASE RESISTANCE IN AFRICAN CATFISH (Clarias gariepinus)

Suprapto

Alimudddin

Nuryati³

et al. 2017

Indonesian Aquaculture Journal

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One of the important issues in catfish farming is motile aeromonad septicaemia (MAS) disease caused by the bacterium Aeromonas hydrophila. This study aimed to find the MHC-II marker potential for marker-based selection to generate MAS disease resistance of African catfish. PCR method was applied to identify catfish (body length: 7-8 cm) population that have MHC-II marker. Fish with and without the marker were then challenged by intraperitonially injecting of 0.1 mL/fish with A. hydrophila (10 5 cfu/mL). The results showed that the survival of fish having MHC-II marker (77.50 ± 4.00%) was higher than that of fish without the marker (53.33 ± 4.77%). Fish carrying MHC-II marker fish has also higher total erythrocytes, total leukocytes, phagocytic activity, and hematocrit levels than that of fish without the marker. The PCR results using specific primer for MHC-II showed a specific DNA band of 426 bp in fish having the marker, while there were no DNA bands in fish without the marker. Results of the PCR analyses showed that the percentage of progenies carrying MHC-II marker was 80%, while progenies from broodstock without the marker was 0%; this indicated that MHC-II marker could be inherited to the offsprings. Thus, the MHC-II marker could be used as a molecular marker of MAS disease resistance catfish.

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“…Metode lain yang dapat diterapkan adalah melalui penggabungan metode konvensional dan modern seperti seleksi menggunakan atau berdasarkan pada marka yang bersifat molekuler. Menurut Pang et al (2013), metode seleksi genetik marker assisted selection (MAS) dapat digunakan untuk menghasilkan ikan yang resisten terhadap penyakit.…”

Section: Performa Pembesaran Ikan Mas Rajadanu (Cyprinus Carpio) unclassified

PERFORMA PEMBESARAN IKAN MAS RAJADANU (Cyprinus carpio) GENERASI KETIGA HASIL SELEKSI “WALKBACK”

Himawan¹,

Syahputra²,

Ariyanto³

2017

Jurnal Riset Akuakultur

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ABSTRAKTimbulnya wabah penyakit KHV (Koi herpesvirus) pada budidaya ikan mas telah menurunkan produksi ikan mas nasional secara signifikan. Beberapa langkah dilakukan untuk mengatasi penyakit tersebut, salah satunya pembentukan ikan mas Rajadanu tahan KHV melalui seleksi. Dalam rangka memperoleh varietas unggul ikan mas tahan KHV pada tahun 2014 telah dilakukan pembentukan populasi F-3 melalui program selective breeding dengan metode walkback selection. Tujuan penelitian ini adalah menganalisis performa pertumbuhan ikan mas Rajadanu generasi ketiga hasil seleksi melalui uji skala lapang pada fase pembesaran, serta kegiatan uji tantang pada skala laboratorium. Benih F-3 merupakan hasil pemijahan F-1 betina dan F-2 jantan hasil seleksi positif MHC (Major Histocompatibility Complex), sedangkan kontrol berupa benih ikan mas Majalaya dari pembudidaya, masing-masing tiga ulangan. Uji tantang KHV dilakukan di akuarium berukuran 60 cm x 40 cm x 40 cm di hatchery ikan mas dengan suhu permissive 21°C melalui metode kohabitasi. Hasil penelitian menunjukkan bahwa populasi F-3 ikan mas Rajadanu hasil seleksi memiliki performa pertumbuhan relatif lebih baik 6,20% dibandingkan dengan kontrol pada karakter panjang total (18,14±4,90 cm), 5,80% lebih baik pada karakter panjang standar (14,76 ± 4,39 cm); 38,39% lebih baik pada karakter bobot rata-rata (393,98 ± 3,53 g); 16,83% pada karakter laju pertumbuhan spesifik (2,29 ± 0,04%); dan 3,80% pada karakter sintasan (49,13 ± 6,88%). Hasil uji tantang KHV menunjukkan sintasan populasi F-3 lebih tinggi (96,6 ± 3,3%) dibandingkan dengan kontrol (51,1 ± 8,3%).

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Major histocompatibility complex class IIA and IIB genes of Nile tilapia Oreochromis niloticus: Genomic structure, molecular polymorphism and expression patterns

Cited by 119 publications

References 36 publications

Untitled

Untitled

MHC-II MARKER POTENTIAL LINKED TO MOTILE AEROMONAD SEPTICAEMIA DISEASE RESISTANCE IN AFRICAN CATFISH (Clarias gariepinus)

PERFORMA PEMBESARAN IKAN MAS RAJADANU (Cyprinus carpio) GENERASI KETIGA HASIL SELEKSI “WALKBACK”

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